165,839 results

1. pAD-sfGFP(Sp)-Pman

   Plasmid

   #133833

   Purpose

   Fluorescent protein sfGFP(Sp); under control of mannose inducible promoter.

   Depositor

   Oscar Kuipers

   Article

   Yi et al Environ Microbiol Rep. 2018 Feb;10(1):57

   Insert

   sfGFP(Sp)
   

   Use

   E.coli/bacillus spp. shuttle vector

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Pman

   Available Since

   Nov. 4, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. HA-HIF2alpha-pcDNA3

   Plasmid

   #18950

   Depositor

   William Kaelin

   Article

   Kondo et al Cancer Cell. 2002 Apr . 1(3):237-46.

   Insert

   Hypoxia inducible factor 2 alpha (EPAS1 Human)
   

   Use

   Tags

   HA-tag

   Expression

   Mammalian

   Mutation

   wt cDNA

   Promoter

   Available Since

   Aug. 20, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCalN-ddGFP-A

   Plasmid

   #40290

   Depositor

   Robert Campbell

   Article

   Alford et al ACS Synth Biol. 2012 Dec 21;1(12):569-75

   Insert

   Calnexin (CANX Human)
   

   Use

   Tags

   ddGFP-A

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Sept. 21, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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4. pMIEG3-JunDN

   Plasmid

   #40350

   Depositor

   Alexander Dent

   Article

   Wang et al J Immunol. 2005 Feb 15;174(4):2098-105.

   Insert

   Jun dominant negative (JUN Human)
   

   Use

   Retroviral

   Tags

   HA and IRES eGFP

   Expression

   Mammalian

   Mutation

   first 122 amino acids deleted

   Promoter

   Available Since

   Sept. 28, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-myc-FLNa WT

   Plasmid

   #8982

   Depositor

   John Blenis

   Article

   Woo et al Mol Cell Biol. 2004 Apr . 24(7):3025-35.

   Insert

   Filamin A (FLNA Human)
   

   Use

   Tags

   myc

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Oct. 11, 2005

   Availability

   Academic Institutions and Nonprofits only

   

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6. pGEX6P1-mClover3-anti-mouse-IgG(kappa)-nanobody

   Plasmid

   #138128

   Purpose

   Expresses GST-tagged mClover3-anti-mouse-IgG(kappa)-nanobody in E. coli cells.

   Depositor

   Kazuhisa Nakayama

   Article

   Katoh et al Mol Biol Cell. 2020 Jul 29:mbcE20040250.

   Insert

   mClover3-anti-mouse-IgG(kappa)-nanobody
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   July 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. pGP-AAV-syn-FLEX-jGCaMP8s-WPRE

   Plasmid

   #162377

   Purpose

   AAV-mediated expression of ultrafast protein calcium sensor under the Syn promoter, Cre-dependent expression

   Depositor

   GENIE Project

   Article

   Zhang et al Janelia Research Campus, Online Resource

   Has Service

   AAV Retrograde, AAV1, and AAV9

   Insert

   jGCaMP8s
   

   Use

   AAV and Cre/Lox

   Tags

   6xHis

   Expression

   Mammalian

   Mutation

   S26M F286Y Q315H

   Promoter

   Synapsin

   Available Since

   Nov. 30, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pT3TS-spCas9 ABE-Umax

   Plasmid

   #222138

   Purpose

   Adenine base editor with modified TadA variant for A-to-G base editing in zebrafish

   Depositor

   Gaurav Varshney

   Article

   Qin et al Nat Commun. 2024 Jul 4;15(1):5613. doi:

   Insert

   Modified TadA-nSpCas9 (cas9 Synthetic)
   

   Use

   CRISPR; Zebrafish expression

   Tags

   bipartite NLS

   Expression

   Mutation

   Promoter

   T3

   Available Since

   Aug. 19, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. p11-LacY-wtx1

   Plasmid

   #69056

   Purpose

   The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

   Depositor

   Huimin Zhao

   Article

   Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

   Inserts

   ccdB (ccdB )
   lacY (lacY )
   

   Use

   Tags

   A177C

   Expression

   Mutation

   Promoter

   pBAD and pLac

   Available Since

   Sept. 30, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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10. pNeg-Ma-barnase-TAG3-TAG45

    Plasmid

    #197572

    Purpose

    Negative selection plasmid for selecting ncAA-encoding Methanomethylophilus alvus Pyl-RS mutants. Expresses 2xTAG-codon interrupted barnase gene and M. alvus Pyl-tRNA(6). p15a origin of replication.

    Depositor

    Ryan Mehl

    Article

    Avila-Crump et al ACS Chem Biol. 2022 Dec 16;17(12):3458-3

    Inserts

    Barnase - 2xTAG
    M. alvus Pyl-tRNA (6)
    

    Use

    Tags

    none

    Expression

    Bacterial

    Mutation

    3TAG and 45TAG

    Promoter

    araC and lpp

    Available Since

    March 21, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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