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AID-mTagBFP2-loxP_myo2_neoR-loxP

Plasmid

#194055

Purpose

Dual-selection cassette plasmid for knocking-in AID-mTagBFP2 into the C. elegans genome

Depositor

Kota Mizumoto

Article

Kurashina et al STAR Protoc. 2023 Jan 13;4(1):102028. do

Insert

IAA17 (AXR3 Mustard Weed)

Tags

codon optimized mTagBFP2

Expression

Worm

Promoter

None

Available Since

Jan. 18, 2023

Availability

Academic Institutions and Nonprofits only

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pZT-C13-L1

Plasmid

#62196

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Left CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pZT-C13-R1

Plasmid

#62197

Purpose

Chr.13/CLYBL TALEN expression vector

Depositor

Jizhong Zou

Article

Cerbini et al PLoS One. 2015 Jan 14;10(1):e0116032. do

Insert

Right CLYBL TALEN

Use

TALEN

Promoter

CMV

Available Since

April 22, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-TRKC-V5/HIS

Plasmid

#205615

Purpose

Expression of human TRKC receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

TRKC receptor tyrosine kinase (NTRK3 Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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Lenti_EFS-Cas9-P2A-HygR

Plasmid

#164134

Purpose

Lentiviral construct for the expression of SpCas9 driven by EFS promoter in mammalian cells

Depositor

Hyongbum Kim

Article

Park et al Cell. 2021 Jan 28. pii: S0092-8674(21)00

Insert

SpCas9 (cas9 Synthetic)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EFS

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pLV-CMV-LoxP-DsRed-LoxP-eGFP

Plasmid

#65726

Purpose

Functions as a genetic Cre reporter that switches from DsRed expression to eGFP expression upon presence of Cre recombinase.

Depositor

Jacco van Rheenen

Article

Zomer et al Cell. 2015 May 21;161(5):1046-57. doi: 1

Insert

LoxP-DsRed-LoxP-eGFP

Use

Cre/Lox and Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

July 6, 2015

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-TYMS_sgRNA1

Plasmid

#201628

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

TYMS (TYMS Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-RYK-V5/HIS

Plasmid

#205610

Purpose

Expression of human RYK receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

RYK receptor tyrosine kinase (RYK Human)

Tags

V5-His

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV-EF1a-DIO-GRAB_g5-HT3.0

Plasmid

#208726

Purpose

Expresses the green 5-HT sensor GRAB_g5-HT3.0 in neurons in the presence of Cre recombinase

Depositor

Yulong Li

Article

Deng et al Nat Methods. 2024 Apr;21(4):692-702. doi

Insert

Green fluorescent 5-HT sensor GRAB_g5-HT3.0

Use

AAV

Promoter

EF1a

Available Since

Oct. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pCCL-dNTP63Promoter-FLuc-CMV-RLuc-dsRed2

Plasmid

#215326

Purpose

Lentiviral mammalian vector with firefly luciferase reporter for dNTP63 expression; renilla luciferase and dsRed2 reporter under CMV promoter.

Depositor

Rob Hynds

Article

Orr et al Am J Physiol Lung Cell Mol Physiol. 2024

Insert

dNTP63 Promoter

Use

Lentiviral and Luciferase

Expression

Mammalian

Promoter

dNTP63

Available Since

July 25, 2024

Availability

Academic Institutions and Nonprofits only

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VPS13C^mClover3

Plasmid

#118760

Purpose

VPS13C internally tagged with mClover3 at residue 1914.

Depositor

Pietro De Camilli

Article

Kumar et al J Cell Biol. 2018 Oct 1;217(10):3625-363

Insert

VPS13C (VPS13C Human)

Tags

mClover3 inserted at position aa 1914

Expression

Mammalian

Available Since

Nov. 16, 2018

Availability

Academic Institutions and Nonprofits only

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dCas9-VPR_P2A_mCherry

Plasmid

#154193

Purpose

Lentiviral expression plasmid encoding dCas9-VPR and a P2A linked mCherry marker

Depositor

Anna Obenauf

Article

Umkehrer et al Nat Biotechnol. 2020 Jul 27. pii: 10.103

Insert

sp-dCas9-VPR_P2A_mCherry

Use

Lentiviral

Expression

Mammalian

Mutation

D10A, H840A

Promoter

EF1as

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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c-myc-PT3EF1a

Plasmid

#92046

Purpose

Expresses c-Myc in mammalian cells

Depositor

Xin Chen

Article

Liu et al Hepatology. 2017 Mar 30. doi: 10.1002/he

Insert

C-MYC (MYC Human)

Expression

Mammalian

Promoter

EF-1a

Available Since

July 15, 2017

Availability

Academic Institutions and Nonprofits only

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pDEST47-CYFIP1.GFP

Plasmid

#109139

Purpose

GFP-tagged CYFIP1 expression construct

Depositor

Josef Kittler

Article

Pathania et al Transl Psychiatry. 2014 Mar 25;4:e374. d

Insert

CYFIP1 (CYFIP1 Human)

Tags

GFP

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 7, 2019

Availability

Academic Institutions and Nonprofits only

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GFP-rab11 WT

Plasmid

#12674

Depositor

Richard Pagano

Article

Choudhury et al J Clin Invest. 2002 Jun . 109(12):1541-5

Insert

RAB11A (Homo sapiens) (RAB11A Human)

Tags

GFP and HA

Expression

Mammalian

Available Since

Oct. 16, 2006

Availability

Academic Institutions and Nonprofits only

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pmRFP-N1 human cofilin S3E

Plasmid

#50858

Purpose

expresses psuedo-phosporylated, non-activatable cofilin fused to mRFP in mammalian cells

Depositor

James Bamburg

Article

Garvalov et al J Neurosci. 2007 Nov 28;27(48):13117-29.

Insert

cofilin 1 S3E (CFL1 Human)

Tags

mRFP

Expression

Mammalian

Mutation

S3E psuedo-phosporylated, non-activatable

Promoter

CMV

Available Since

Feb. 20, 2014

Availability

Academic Institutions and Nonprofits only
pAAV-pTH-iCre-WPREpA

Plasmid

#213130

Purpose

Truncated rat TH promoter expressing iCre

Depositor

Ulrik Gether

Article

Lycas et al Cell Rep. 2022 Sep 27;40(13):111431. doi

Insert

iCre

Use

AAV and Mouse Targeting

Tags

None

Expression

Mammalian

Promoter

Truncated TH promoter from rat (Rattus norvegicus…

Available Since

Feb. 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLM-mCitrine-Sox2

Plasmid

#23242

Depositor

Michel Sadelain

Article

Papapetrou et al Proc Natl Acad Sci U S A. 2009 Aug 4. 10

Insert

mCitrine_2A_SOX2 (SOX2 Human, synthetic fluorescent protein)

Use

Lentiviral

Available Since

Feb. 5, 2010

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-mRuby3-6xFLAG

Plasmid

#161613

Purpose

Cell morphology marker construct. Contains a red fluorescent protein mRuby3 and six FLAG tags.

Depositor

Edward Boyden

Article

Linghu et al Cell. 2020 Nov 17. pii: S0092-8674(20)31

Insert

mRuby3-6xFLAG

Use

AAV

Expression

Mammalian

Mutation

N/A

Promoter

Human synapsin 1 promoter

Available Since

March 25, 2021

Availability

Academic Institutions and Nonprofits only

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pCMV-GalT-mCherry

Plasmid

#214271

Purpose

Mammalian expression of GalT-mCherry (Golgi marker)

Depositor

Shinya Tsukiji

Article

Ajiki et al RSC Chem Biol. 2024 Apr 24;5(6):544-555.

Insert

GalT(1-82)-mCherry (B4GALT1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 21, 2024

Availability

Academic Institutions and Nonprofits only

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