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pDONR221_SLC26A1

Plasmid

#131972

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC26A1 (SLC26A1 Human)

Expression

Mammalian

Available Since

Oct. 18, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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AAV_CMV_CCR6-Nanoluc-V5

Plasmid

#199672

Purpose

expresses CCR6-NanoLuc-V5 in mammalian cells, AAV vector

Depositor

Alice Ting

Article

Lee et al Nat Methods. 2023 May 15. doi: 10.1038/s

Insert

CCR6 (CCR6 Human)

Use

AAV

Tags

NanoLuc and V5

Expression

Mammalian

Available Since

May 15, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC12A6

Plasmid

#131956

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC12A6 (SLC12A6 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pME-Luciferase-P2A-H2A-mCherry (JDW 926)

Plasmid

#224488

Purpose

Gateway compatible middle entry clone containing a Luciferase P2A H2A-mCherry dual reporter (Luciferase/nuclear red fluorescent reporter)

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

Luciferase-P2A-H2A-mCherry

Use

Luciferase

Available Since

Sept. 30, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC5A9

Plasmid

#132230

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC5A9 (SLC5A9 Human)

Expression

Mammalian

Available Since

Nov. 8, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC25A47

Plasmid

#131983

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC25A47 (SLC25A47 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pBEL1200

Plasmid

#138526

Purpose

For expresson of mlaFEDCB operon from E. coli. N-terminal his tag on MlaD, araBAD promoter.

Depositor

Gira Bhabha, Damian Ekiert

Article

Ekiert et al Cell. 2017 Apr 6;169(2):273-285.e17. doi

Insert

mlaFEDCB (mlaF, mlaE, mlaD, mlaC, mlaB E. coli)

Tags

N-terminal His tag and TEV cleavage site on MlaD

Expression

Bacterial

Promoter

araBAD

Available Since

March 6, 2020

Availability

Academic Institutions and Nonprofits only

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LENTICRISPR-ALDOA_sgRNA1

Plasmid

#201590

Purpose

CRISPR/Cas9-mediated gene knock-out

Depositor

Alexis Jourdain, Vamsi Mootha

Article

Skinner et al Nat Metab. 2023 May;5(5):765-776. doi: 1

Insert

ALDOA (ALDOA Human)

Use

CRISPR and Lentiviral

Available Since

June 22, 2023

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC17A8

Plasmid

#131961

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC17A8 (SLC17A8 Human)

Expression

Mammalian

Available Since

Oct. 21, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pLKO.1-mTagBFP2-rMPC1 shRNA

Plasmid

#229016

Purpose

Expression of an shRNA construct that knocks down rat MPC1. This plasmid also encodes a blue fluorescent protein tag to verify transfection

Depositor

Ghazaleh Ashrafi

Article

Tiwari et al Sci Adv. 2024 Nov 15;10(46):eadp7423. do

Insert

MPC1 (Mpc1 Rat)

Use

Lentiviral

Tags

mTagBFP2

Expression

Mammalian

Promoter

U6

Available Since

Jan. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pDONR221_SV2B

Plasmid

#132281

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SV2B (SV2B Human)

Expression

Mammalian

Available Since

May 19, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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pcDNA3.1-PDGFRA-V5/HIS

Plasmid

#234754

Purpose

Expression of human PDGFRA receptor tyrosine kinase in mammalian cells

Depositor

Pavel Krejčí

Article

Gudernova et al Elife. 2017 Feb 15;6. pii: e21536. doi:

Insert

PDGFRA receptor tyrosine kinase, full length, wildtype (PDGFRA Human)

Tags

V5/His

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

April 14, 2025

Availability

Academic Institutions and Nonprofits only

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pMRXIP-GFP-TRPML1(PHD)

Plasmid

#221752

Purpose

Stable expression of GFP-TRPML1(PHD) in mammalian cells

Depositor

Noboru Mizushima

Article

Shinoda et al Elife. 2024 Jun 4;12:RP92189. doi: 10.75

Insert

TRPML1 (MCOLN1 Human)

Use

Retroviral

Tags

GFP

Expression

Mammalian

Mutation

tandem TRPML1(PHD) (amino acids 1–69)

Available Since

Aug. 27, 2024

Availability

Academic Institutions and Nonprofits only

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pDONR221_SLC39A6

Plasmid

#132153

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC39A6 (SLC39A6 Human)

Expression

Mammalian

Available Since

Oct. 22, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pMXS-IRES-Blast FLVCR1b-3X FLAG

Plasmid

#218536

Purpose

human FLVCR1b with C-terminal 3X_FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Mutation

Alternative isoform

Available Since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a-3X FLAG

Plasmid

#218534

Purpose

human FLVCR1/FLVCR1a with C-terminal 3x-FLAG cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Tags

FLAG (3X)

Expression

Mammalian

Available Since

June 12, 2024

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast FLVCR1/FLVCR1a

Plasmid

#218533

Purpose

human FLVCR1/FLVCR1a cDNA

Depositor

Kivanc Birsoy

Article

Kenny et al Cell Metab. 2023 Jun 6;35(6):1057-1071.e

Insert

FLVCR1 (FLVCR1 Human)

Use

Retroviral

Expression

Mammalian

Available Since

May 7, 2024

Availability

Academic Institutions and Nonprofits only

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GFP(N-glyc)-Tac-TC

Plasmid

#162496

Purpose

Expresses partial length Tac (TMD and cytosolic tail) with GFP tag in the N-terminus in mammalian cells, and there is one N-glycosylation site in GFP tag.

Depositor

Lei Lu

Article

Sun et al J Biol Chem. 2020 Aug 21. pii: RA120.014

Insert

partial length Tac (TMD and cytosolic tail) (IL2RA Human)

Tags

GFP

Expression

Mammalian

Mutation

Tac is in partial length with its TMD and cysotol…

Available Since

Jan. 29, 2021

Availability

Academic Institutions and Nonprofits only

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pDONR221_TMEM104

Plasmid

#132262

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

TMEM104 (TMEM104 Human)

Expression

Mammalian

Available Since

Nov. 11, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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pDONR221_SLC46A2

Plasmid

#132203

Purpose

Gateway entry clone with codon-optimized ORF sequence for subcloning into custom expression vectors

Depositor

RESOLUTE Consortium, Giulio Superti-Furga

Article

Superti-Furga et al Nat Rev Drug Discov. 2020 Jul;19(7):429-

Insert

SLC46A2 (SLC46A2 Human)

Expression

Mammalian

Available Since

Oct. 28, 2019

Availability

Industry, Academic Institutions, and Nonprofits

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