We narrowed to 14,291 results for: SHI

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pAAV-CaMKIIa-ChRmine-eYFP-Kv2.1-WPRE

Plasmid

#130989

Purpose

AAV vector to drive the expression of soma-targeted ChRmine-eYFP under the control of CamKIIa promoter

Depositor

Karl Deisseroth

Article

Marshel et al Science. 2019 Aug 9;365(6453). pii: scie

Insert

ChRmine

Use

AAV

Tags

EYFP-Kv2.1

Promoter

CaMKIIa

Available Since

Sept. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 a22-end_I132S-S212N

Plasmid

#113952

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, I132S and S212N su…

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pAG247

Plasmid

#33200

Depositor

David Bartel

Article

Garcia et al Nat Struct Mol Biol. 2011 Sep 11;18(10):

Insert

LRIG1 (LRIG1 Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Promoter

SV40 early enhancer/promoter

Available Since

Jan. 10, 2012

Availability

Academic Institutions and Nonprofits only
TRCN0000072254

Plasmid

#78161

Purpose

Luciferase control

Depositor

William Hahn

Article

Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

Insert

Luciferase

Use

RNAi

Promoter

hU6

Available Since

June 22, 2016

Availability

Academic Institutions and Nonprofits only
His-GST-Cys-M87 Spastin

Plasmid

#184804

Purpose

Bacterial expression of M87 Spastin (A2C mutation)

Depositor

James Hurley

Article

Cada et al Proc Natl Acad Sci U S A. 2022 Jul 19;11

Insert

M87 Spastin (SPAST Human)

Tags

His6-GST

Expression

Bacterial

Mutation

A2C

Promoter

T7

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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pRK5 Fyn SH2:R176E

Plasmid

#16035

Depositor

Filippo Giancotti

Article

Mariotti et al J Cell Biol. 2001 Oct 29. 155(3):447-58.

Insert

c-Fyn (FYN Human)

Expression

Mammalian

Mutation

SH2 point mutant: R176E

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only
pGEX p85a(N)-SH2

Plasmid

#46464

Depositor

Leszek Kotula, Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

p85a(N) (PIK3R1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 321-434

Promoter

Tac

Available Since

July 23, 2013

Availability

Academic Institutions and Nonprofits only

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S100B_pcDNA6.2/EmGFP-Bsd

Plasmid

#176952

Purpose

Mammalian expression vector encoding S100B and EmGFP-Bsd

Depositor

Roger Reeves

Article

Moyer et al Dis Model Mech. 2023 Apr 1;16(4):dmm0497

Insert

S100B (S100B Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 19, 2022

Availability

Academic Institutions and Nonprofits only

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pMXS-IRES-Blast SHMT2res

Plasmid

#106301

Purpose

Expresses SHMT2

Depositor

Richard Possemato

Article

Minton et al Mol Cell. 2018 Feb 15;69(4):610-621.e5.

Insert

serine hydroxymethyltransferase 2 (SHMT2 Human)

Use

Retroviral

Expression

Mammalian

Mutation

Silent mutations destroy sgRNA targeting sites

Available Since

April 30, 2018

Availability

Academic Institutions and Nonprofits only

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TOPO UBC

Plasmid

#68456

Purpose

TOPO construct expressing UBC for generation of GMAP-compatible promoter

Depositor

Tyler Jacks

Article

Akama-Garren et al Sci Rep. 2016 Feb 18;6:16836. doi: 10.10

Insert

Ubiquitin C Promoter (UBC Human)

Use

Gmap

Expression

Bacterial

Promoter

Plac

Available Since

Feb. 22, 2016

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-Muc5b D1 (murine) with cleavable His tag

Plasmid

#181814

Purpose

Expresses murine Muc5b D1 with cleavable His tag, residues 50-426

Depositor

Deborah Fass

Article

Reznik et al Cell. 2022 Oct 27;185(22):4206-4215.e11.

Insert

Muc5b D1 (Muc5b Mouse)

Tags

TEV cleavage site followed by 6xHistidine tag

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 31, 2022

Availability

Academic Institutions and Nonprofits only

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sgRNA BRCA1 E638K

Plasmid

#139327

Purpose

Plasmid expressing a sgRNA to introduce BRCA1 E638K using base editing

Depositor

Alberto Ciccia

Article

Billon et al Cell Rep. 2020 Mar 10;30(10):3280-3295.e

Insert

sgRNA to insert BRCA1 E638K using base editing

Expression

Mammalian

Promoter

U6

Available Since

May 22, 2020

Availability

Academic Institutions and Nonprofits only

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pKT2/CAGXSP/PalmNanoLuc

Plasmid

#182969

Purpose

Stably express PalmNanoLuc in mammalian cells via the sleeping beauty transposon system.

Depositor

Masamitsu Kanada

Article

Perez et al Adv Genet (Hoboken). 2022 Jan 19;3(1):21

Insert

PalmNanoLuc

Expression

Mammalian

Mutation

N/A

Available Since

April 20, 2022

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-Flag-natT1R2 FS10

Plasmid

#113956

Purpose

mammalian expression plasmid for FLAG-tagged human T1R2 with signal peptide of influenza hemagglutinin; expression-enhanced variant

Depositor

Erik Procko

Article

Park et al J Biol Chem. 2019 Feb 5. pii: RA118.0061

Insert

T1R2 (TAS1R2 Human)

Tags

FLAG and Signal/leader sequence from influenza he…

Expression

Mammalian

Mutation

delete N-terminal 21 residues, S212N substitution…

Promoter

cmv

Available Since

Feb. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pCDNA3_shRNA resistant PKA-Cα-mEGFP

Plasmid

#114143

Purpose

Mammalian expression of the mouse PKA catalytic subunit α C-terminally tagged by monomeric mEGFP. Silent mutations were introduced to make it resistant to shRNA knock-down.

Depositor

Haining Zhong

Article

Tillo et al Cell Rep. 2017 Apr 18;19(3):617-629. doi

Insert

mouse PKA-Cα-mEGFP with silent mutations resisting shRNA knockdown

Expression

Mammalian

Available Since

Oct. 1, 2018

Availability

Academic Institutions and Nonprofits only

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20XUAS IVS Jaws mCherry_tr

Plasmid

#111549

Purpose

Optogenetics in Drosophila: UAS-induced expression of Jaws mCherry

Depositor

Vivek Jayaraman

Article

Chuong et al Nat Neurosci. 2014 Aug;17(8):1123-9. doi

Insert

Jaws_mCherry_trafficked

Expression

Insect

Mutation

wt

Promoter

hsp70

Available Since

June 26, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA1-Bystin

Plasmid

#11869

Depositor

Michiko Fukuda

Article

Suzuki et al Proc Natl Acad Sci U S A. 1998 Apr 28. 9

Insert

Bystin (BYSL Human)

Expression

Mammalian

Available Since

Sept. 1, 2006

Availability

Academic Institutions and Nonprofits only
pGEX Fes-SH2

Plasmid

#46426

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

Fes (FES Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 396-497

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pCS3-MT-BX/p14ARF

Plasmid

#78761

Purpose

To overexpress p14ARF in Mammalian Cells

Depositor

Jaewhan Song

Article

Ko et al J Natl Cancer Inst. 2012 Nov 7;104(21):1

Insert

ARF (CDKN2A Human)

Tags

6xMYC

Expression

Mammalian

Promoter

CMV IE94

Available Since

June 9, 2016

Availability

Academic Institutions and Nonprofits only
GFP-Braf R188L

Plasmid

#118330

Purpose

Expression of human Braf

Depositor

Philip Stork

Article

Li et al J Biol Chem. 2013 Sep 20;288(38):27646-5

Insert

Braf R188L (BRAF Human)

Tags

GFP

Expression

Mammalian

Mutation

R188L

Promoter

CMV

Available Since

Nov. 9, 2018

Availability

Academic Institutions and Nonprofits only

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