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pDONR221-ARL1-no stop

Plasmid

#67317

Purpose

hArl1 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL1 (ARL1 Human)

Use

Gateway Cloning

Expression

Bacterial

Mutation

bp 231 C to T; silent mutation

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pDONR221-ARL3-no stop

Plasmid

#67321

Purpose

hArl3 without stop codon in Gateway Entry vector

Depositor

Richard Kahn

Article

Kerr et al Cell Logist. 2015 Sep 21;5(3):e1090523.

Insert

ARL3 (ARL3 Human)

Use

Gateway Cloning

Expression

Bacterial

Promoter

None

Available Since

Oct. 5, 2015

Availability

Academic Institutions and Nonprofits only
pASKt15C+SrtA2

Plasmid

#64984

Purpose

Expresses the E105K/E108A mutant of Staphylococcus aureus sortase A in E. coli

Depositor

Teruyuki Nagamune

Article

Hirakawa et al Biotechnol J. 2015 Apr 10. doi: 10.1002/

Insert

sortase A

Tags

His6 tag and S tag

Expression

Bacterial

Mutation

deleted amino acids 1-59, E105K, E108A

Promoter

tetracycline promoter/operator

Available Since

June 9, 2015

Availability

Academic Institutions and Nonprofits only

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PS-CFP2-MCPB1-N-18

Plasmid

#57237

Purpose

Localization: Actin Capping Protein , Excitation: 400 / 490, Emission: 468 / 511

Depositor

Michael Davidson

Insert

MCPB1 (Capzb Mouse)

Tags

PS-CFP2

Expression

Mammalian

Promoter

CMV

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2-MCPB2-N-18

Plasmid

#57474

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MCPB2 (Capzb Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 27, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos2-TBRII-N-18

Plasmid

#57425

Purpose

Localization: Membrane Receptor, Excitation: 505 / 569, Emission: 516 / 581

Depositor

Michael Davidson

Insert

TBRII (TGFBR2 Human)

Tags

mEos2

Expression

Mammalian

Mutation

H385N

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mEos3.2-MCPB1-N-18

Plasmid

#57472

Purpose

Localization: Actin Capping Protein , Excitation: 507 / 572, Emission: 516 / 580

Depositor

Michael Davidson

Insert

MCPB1 (Capzb Mouse)

Tags

mEos3.2

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2015

Availability

Industry, Academic Institutions, and Nonprofits

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mTurquoise-TBRII-N-18

Plasmid

#55582

Purpose

Localization: Membrane Receptor, Excitation: 434, Emission: 474

Depositor

Michael Davidson

Insert

TBRII (TGFBR2 Human)

Tags

mTurquoise

Expression

Mammalian

Mutation

H385N

Promoter

CMV

Available Since

Oct. 2, 2014

Availability

Industry, Academic Institutions, and Nonprofits

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pChlr-3CQ

Plasmid

#52147

Purpose

Encodes module 3 with amino acids 12C-16Q for recognition of nt 6A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 97-132 (PUM1 Human)

Expression

Bacterial

Available Since

May 5, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-4NQ

Plasmid

#52153

Purpose

Encodes module 4 with amino acids 12N-16Q for recognition of nt 5U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 133-168 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-5CQ

Plasmid

#52159

Purpose

Encodes module 5 with amino acids 12C-16Q for recognition of nt 4A

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 169-204 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-6NQ

Plasmid

#52165

Purpose

Encodes module 6 with amino acids 12N-16Q for recognition of nt 3U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 205-240 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pChlr-8NQ

Plasmid

#52173

Purpose

Encodes module 8 with amino acids 12N-16Q for recognition of nt 1U

Depositor

Huimin Zhao

Article

Abil et al J Biol Eng. 2014 Mar 1;8(1):7.

Insert

Pumilio homology domain amino acids 284-319 (PUM1 Human)

Expression

Bacterial

Available Since

April 28, 2014

Availability

Academic Institutions and Nonprofits only

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pClneoFLAG human Dendrin

Plasmid

#47934

Purpose

expresses FLAG tagged human Dendrin in mammalian cells

Depositor

Yutaka Hata

Article

Kawata et al J Biochem. 2006 May;139(5):931-9.

Insert

Dendrin-1 (DDN Human)

Tags

FLAG

Expression

Mammalian

Mutation

contains amino acids 2-711 of human Dendrin

Promoter

CMV

Available Since

Sept. 9, 2013

Availability

Academic Institutions and Nonprofits only
pGEX chimerin-SH2

Plasmid

#46415

Depositor

Bruce Mayer

Article

Machida et al Mol Cell. 2007 Jun 22;26(6):899-915.

Insert

chimerin (CHN1 Human)

Tags

GST and PreScission

Expression

Bacterial

Mutation

contains amino acids 44-147

Promoter

Tac

Available Since

July 10, 2013

Availability

Academic Institutions and Nonprofits only

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pSG5-FLAG-CaMKKalpha RP deletion

Plasmid

#33328

Depositor

Anthony Means

Article

Green et al Cell Signal. 2011 Dec;23(12):2005-12. Ep

Insert

Calcium/Calmodulin dependent protein kinase kinase alpha (Camkk1 Rat)

Tags

FLAG

Expression

Mammalian

Mutation

Deleted RP domain (amino acids 167-189 missing)

Promoter

SV40

Available Since

April 10, 2012

Availability

Academic Institutions and Nonprofits only
hs casper RhoL-V12

Plasmid

#17582

Depositor

Denise Montell

Insert

RhoL (RhoL Fly)

Expression

Insect

Mutation

V12

Available Since

April 3, 2008

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 1-373 pBacPAK8

Plasmid

#15328

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 1-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-621 pBacPAK8

Plasmid

#15329

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-621

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only
FLAG-ELL 45-373 pBacPAK8

Plasmid

#15330

Depositor

Joan Conaway, Ronald Conaway

Article

Kong et al Proc Natl Acad Sci U S A. 2005 Jul 19. 1

Insert

eleven nineteen lysine rich in leukemia (ELL Human)

Use

Baculovirus construction

Tags

FLAG

Mutation

Amino acids 45-373

Available Since

Aug. 15, 2007

Availability

Academic Institutions and Nonprofits only