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Plasmid#220643PurposeAiE2128m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
AiP20013 - pAAV-AiE2674m-minBG-SYFP2-WPRE3-BGHpA
Plasmid#230556PurposeAiE2674m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable sinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pCDH-anti-mCherry(LaM6) PAGER(Gq)
Plasmid#230016PurposeLentiviral vector expressing anti-mCherry PAGER(Gq) in mammalian cellsDepositorInsertMT1-LaM6-TEVcs-ALFA-hM1D
UseLentiviralExpressionMammalianMutationW442A on hM1DPromoterCMVAvailable sinceJan. 6, 2025AvailabilityAcademic Institutions and Nonprofits only -
PET-28a-YTH domain of YTHDF3
Plasmid#228777PurposeProtein expression plasmid for YTH domain of YTHDF3 in E.Coli.DepositorAvailable sinceJan. 2, 2025AvailabilityAcademic Institutions and Nonprofits only -
SpCas9-NG-CGBE1
Plasmid#198537PurposeExpresses human codon-optimized SpCas9-NG-CGBE1 and blasticidin resistance: EFS promoter-SpCas9-NG-CGBE1-NLS-FLAG-P2A-BSDDepositorInsertSpCas9-NG-CGBE1
UseCRISPR and LentiviralTagsBSD, FLAG, and NLSExpressionMammalianPromoterEFSAvailable sinceNov. 5, 2024AvailabilityAcademic Institutions and Nonprofits only -
pET21b-MBPx-MBPx-SpyTag003
Plasmid#216291PurposeExpresses tandem MBPx linked to-SpyTag003 in bacterial cells. MBPx is a variant of MBP (A312V I317V Δ172-173 Δ175-176) with enhanced affinity for maltose.DepositorInsertMBPx-MBPx-SpyTag003
UseAffinity Reagent/ AntibodyTagsHis6 and SpyTag003ExpressionBacterialPromoterT7 promoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pMV-SB100X[EF1α-copGFP]
Plasmid#218323PurposeDonor plasmid of SB100X Transposon with copGFP mammalian expression cassetteDepositorInsertcopGFP mammalian expression cassette
ExpressionMammalianAvailable sinceOct. 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP1657 - pAAV-AiE2339m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214508PurposeAiE2339m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
pETSUMO2_Bacteroidetes-bGSDM-TEV
Plasmid#222101PurposeFor expression of a mutant bacterial gasdermin (bGSDM) encoded by a Bacteroidetes species with a TEV protease cleavage site for controlled activation.DepositorInsertBacteroidetes-bGSDM-TEV ORF
Tags6xHis-SUMO2ExpressionBacterialMutationTEV protease site replaces residues E242–G248PromoterT7Available sinceJuly 12, 2024AvailabilityAcademic Institutions and Nonprofits only -
AiP1969 - pAAV-AiE2563m-minBG-SYFP2-WPRE-BGHpA
Plasmid#214597PurposeAiE2563m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cellsDepositorInsertSYFP2
UseAAVPromoterminBGAvailable sinceJune 24, 2024AvailabilityAcademic Institutions and Nonprofits only -
CamKII-LGI1-pHmScarlet
Plasmid#185538PurposeExpresses LGI1-pHmScarlet under the CamKII promoterDepositorAvailable sinceMay 3, 2024AvailabilityAcademic Institutions and Nonprofits only -
MRGPRX2-DuET
Plasmid#213345PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
GHSR-DuET
Plasmid#213251PurposeThe DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.DepositorAvailable sinceApril 23, 2024AvailabilityAcademic Institutions and Nonprofits only -
EEF1A2 in pENTR
Plasmid#216585Purposegateway cloning donor vector containing EEF1A2DepositorInsertEEF1A2 (EEF1A2 Human)
UseGateway CloningAvailable sinceMarch 27, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_CCK1.0
Plasmid#208673PurposeExpresses the genetically-encoded fluorescent cholecystokinin (CCK) sensor GRAB_CCK1.0 in neuronsDepositorInsertGPCR activation based cholecystokinin (CCK) sensor GRAB_CCK1.0
UseAAVPromoterhSynAvailable sinceDec. 20, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSLQ11274:pHR-hU6-crTet-HSP-hyperdCas12a-miniVPR-CMV-mCherry
Plasmid#210603Purposeheat shock promoter - activated hyperdCas12a-based transcriptional activatorDepositorInserthyperdCas12a-miniVPR
UseCRISPR and LentiviralPromotertruncated hsp70Available sinceDec. 4, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-GRAB_UCN1.0
Plasmid#208669PurposeExpresses the genetically-encoded fluorescent urocortin (UCN) sensor GRAB_UCN1.0 in a cre-dependent mannerDepositorInsertGPCR activation based urocortin (UCN) sensor GRAB_UCN1.0
UseAAVPromoterhSynAvailable sinceOct. 24, 2023AvailabilityAcademic Institutions and Nonprofits only -
PET-eSpCas9-NLS-6xHis
Plasmid#207379PurposeExpression of increased-fidelity (i.e. high-fidelity) SpCas9 variant in bacterial cellsDepositorInserteSpCas9
UseCRISPRTags6xHisExpressionBacterialMutationK848A, K1003A, R1060APromoterT7Available sinceSept. 25, 2023AvailabilityAcademic Institutions and Nonprofits only -
Tol2_B-act_dCas9-Vp64-T2A-Citrine
Plasmid#200248PurposeZebrafish transgenics; Plasmid encodes dCas9-Vp64 and could be stably integrated in the zebrafish genome using Tol2 transgenesisDepositorPublicationInsertdCas9-Vp64-T2A-Citrine
UseZebrafish expressionAvailable sinceJuly 18, 2023AvailabilityAcademic Institutions and Nonprofits only -
pTRE3G-dCas9-VP64-MS2-VP64-ZsG
Plasmid#192669PurposeDox-inducible expression of dCas9-VP64 with P2A MS2-VP64 and T2A ZsGreen1DepositorInsertdCas9-VP64, MS2-VP64, ZsGreen1
UseCRISPR and LentiviralExpressionMammalianMutationD10A and N863A in Cas9PromoterTRE3GAvailable sinceDec. 15, 2022AvailabilityAcademic Institutions and Nonprofits only