We narrowed to 25,042 results for: Nes

1. pMSCV-PIG-miR-26a-2

   Plasmid

   #64230

   Purpose

   Expresses miR-26a-2 in mammalian cells

   Depositor

   Joshua Mendell

   Article

   Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

   Insert

   miR-26a-2 (MIR26A2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Nov. 13, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRL1058

   Plasmid

   #70686

   Purpose

   Tn5-based plasmid for transposition in Anabaena sp. strain PCC 7120. Bears an oriV, so when excised from a chromosome or megaplasmid it can carry its neighboring sequences when returned to E. coli.

   Depositor

   Peter Wolk

   Article

   Wolk et al Proc Natl Acad Sci U S A. 1991 Jun 15;88

   Insert

   Transposon Tn5-1058
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Dec. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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3. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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4. EPHA2 (3MBW)

   Plasmid

   #25357

   Purpose

   Bacterial expression for structure determination; may not be full ORF

   Depositor

   Cheryl Arrowsmith

   Insert

   epha2.023.326 (EPHA2 Human)
   

   Use

   Tags

   His

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   Aug. 19, 2010

   Availability

   Industry, Academic Institutions, and Nonprofits

5. pMGC

   Plasmid

   #112812

   Purpose

   PCR template vector for amplifying gRNA(F+E)-OstRNA fragment

   Depositor

   Chun Han

   Article

   Poe et al Genetics. 2018 Nov 30. pii: genetics.118

   Insert

   gRNAcore(F+E)-tRNA
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available Since

   Dec. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCaSpeR-4 porcupine (genomic DNA)

   Plasmid

   #37376

   Depositor

   Norbert Perrimon

   Article

   Kadowaki et al Genes Dev. 1996 Dec 15;10(24):3116-28.

   Insert

   porcupine (por Fly)
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available Since

   July 16, 2012

   Availability

   Academic Institutions and Nonprofits only

7. pISH-Emx1

   Plasmid

   #84309

   Purpose

   Use to synthesize riboprobes for in situ hybridization (ISH) probe for Emx1. Not for gene expression.

   Depositor

   Peter Mombaerts

   Article

   Parrilla et al J Comp Neurol. 2016 Oct 1;524(14):2713-3

   Insert

   Emx1 (Emx1 Mouse)
   

   Use

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Nov. 15, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pENTR-fishRab8A[T22N]

   Plasmid

   #24906

   Depositor

   Maxence Nachury

   Article

   Nachury et al Cell. 2007 Jun 15. 129(6):1201-13.

   Insert

   fishRab8A
   

   Use

   Gateway entry vector

   Tags

   Expression

   Mutation

   Changed Threonine 22 to Asparagine

   Promoter

   Available Since

   July 7, 2010

   Availability

   Academic Institutions and Nonprofits only

9. MSCV-PM-BAF180-SH2

   Plasmid

   #41932

   Depositor

   Stephen Elledge

   Article

   Burrows et al Proc Natl Acad Sci U S A. 2010 Aug 10;10

   Insert

   BAF180
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   May 10, 2013

   Availability

   Academic Institutions and Nonprofits only

10. -226bp goosecoid luciferase

    Plasmid

    #17158

    Depositor

    Ken Cho

    Article

    Watabe et al Genes Dev. 1995 Dec 15. 9(24):3038-50.

    Insert

    goosecoid promoter (gsc-a Frog)
    

    Use

    Luciferase

    Tags

    luciferase

    Expression

    Mutation

    Promoter

    Available Since

    June 20, 2008

    Availability

    Academic Institutions and Nonprofits only

11. 1xNLS-pMJ915v2-sfGFP

    Plasmid

    #88919

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. Hy_pMT dati MCS circfirefly_1677

    Plasmid

    #110118

    Purpose

    Expresses a 1677-nt firefly luciferase circular RNA in Drosophila cells

    Depositor

    Jeremy Wilusz

    Article

    Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

    Insert

    Firefly luciferase
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Metallothionein Promoter (pMT)

    Available Since

    May 22, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. pGL3_ctrl_CP-candidate_luc+

    Plasmid

    #86392

    Purpose

    Luciferase validation vector, where candidates are inserted at the position of the core promoter. The candidates basal activity (no enhancer in vector) is reflected by luciferase activity

    Depositor

    Alexander Stark

    Article

    Arnold et al Nat Biotechnol. 2016 Dec 26. doi: 10.103

    Type

    Empty backbone

    Use

    Luciferase

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    March 27, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pCMV-Flag-YAP-P85A

    Plasmid

    #33062

    Depositor

    Kunliang Guan

    Article

    Li et al Genes Dev. 2010 Feb 1;24(3):235-40.

    Insert

    YAP (YAP1 Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    P85A

    Promoter

    Available Since

    Feb. 10, 2012

    Availability

    Academic Institutions and Nonprofits only

15. pGL3con-miR143Bul

    Plasmid

    #21119

    Depositor

    Joshua Mendell

    Article

    Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

    Insert

    hsa-miR-143 sensor
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    May 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. 2xNLS-pMJ915v2-sfGFP

    Plasmid

    #88920

    Purpose

    For expression of modified SpyCas9 in e.coli.

    Depositor

    Jennifer Doudna

    Article

    Staahl et al Nat Biotechnol. 2017 Feb 13. doi: 10.103

    Inserts

    Cas9
    T7
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    May 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pcDNA3.1 Flag YFP hNKCC1 A734C (NT508)

    Plasmid

    #50888

    Purpose

    Expresses human NKCC1 A734C mutant with an N-terminal 3xFLAG-YFP tag in mammalian cells. cDNA is synthetic, containing convenient restriction sites.

    Depositor

    Biff Forbush

    Insert

    human NKCC1 (synthetic) (SLC12A2 Synthetic, Human)
    

    Use

    Tags

    3x Flag and YFP

    Expression

    Mammalian

    Mutation

    A734C in hNKCC1 in NT17

    Promoter

    CMV

    Available Since

    Feb. 6, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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18. pPS1358

    Plasmid

    #8918

    Depositor

    Pamela Silver

    Article

    Kessler et al Genes Dev. 1997 Oct 1. 11(19):2545-56.

    Insert

    HRP1 (HRP1 Budding Yeast)
    

    Use

    Tags

    GFP

    Expression

    Yeast

    Mutation

    Promoter

    Available Since

    Aug. 19, 2005

    Availability

    Academic Institutions and Nonprofits only

    

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19. pTRANS_120

    Plasmid

    #91203

    Purpose

    Transformation, Type: non-T-DNA, Plant Selection: 2x35S:npt II, Viral Replication: none

    Depositor

    Daniel Voytas

    Article

    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.

    Type

    Empty backbone

    Use

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    July 7, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. pGEM cBspII (CT#624)

    Plasmid

    #13940

    Depositor

    Cliff Tabin

    Article

    Hartmann et al Development. 2000 Jul . 127(14):3141-59.

    Insert

    BspII (IBSP Chicken)
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Amino acids 79-257

    Promoter

    Available Since

    Feb. 15, 2007

    Availability

    Academic Institutions and Nonprofits only