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pF(UG) hSyn SYP-deltaTMD SYT7alpha

Plasmid

#179718

Purpose

Lentiviral expression of synaptic vesicle-targeted mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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pF(UG) hSyn SYP-deltaTMD SYT7alpha-HaloTag

Plasmid

#179719

Purpose

Lentiviral expression of synaptic vesicle-targeted Halo tagged mouse Syt7

Depositor

Edwin Chapman

Article

Vevea et al Elife. 2021 Sep 20;10. pii: 67261. doi:

Insert

SYT7 (Syt7 Mouse)

Use

Lentiviral

Availability

Academic Institutions and Nonprofits only

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SNX26-PX (56-172)

Plasmid

#119105

Purpose

Bacterial expression of human phox homology (PX) domain, SNX26-PX (56-172)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX26-PX (56-172)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX28-PX (1-124)

Plasmid

#119107

Purpose

Bacterial expression of human phox homology (PX) domain, SNX28-PX (1-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX28-PX (1-124)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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SNX34-PX (1-130)

Plasmid

#119113

Purpose

Bacterial expression of human phox homology (PX) domain, SNX34-PX (1-130)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

SNX34-PX (1-130)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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IRAS-PX (13-124)

Plasmid

#119118

Purpose

Bacterial expression of human phox homology (PX) domain, IRAS-PX (13-124)

Depositor

Brett Collins

Article

Chandra et al Nat Commun. 2019 Apr 4;10(1):1528. doi:

Insert

IRAS-PX (13-124)

Tags

GST

Expression

Bacterial

Promoter

tac

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B V304A,I367A IRES GFP

Plasmid

#133821

Purpose

Encodes the C2B domain of synaptotagmin 1 with membrane-penetrating residue mutations V304A,I367A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

V304A, I367A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2B D363,365N IRES GFP

Plasmid

#133822

Purpose

Encodes the C2B domain of synaptotagmin 1 with calcium-coordinating ligand mutations D363,365N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D363,365N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A K189-192A IRES GFP

Plasmid

#133823

Purpose

Encodes the C2A domain of synaptotagmin 1 with poly-lysine patch mutations K189-192A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

K189-192A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A D230,232N IRES GFP

Plasmid

#133824

Purpose

Encodes the C2A domain of synaptotagmin 1 with calcium-coordinating ligand mutations D230,232N for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

D230,232N

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pFSW Syt1-C2A M173,F234A IRES GFP

Plasmid

#133825

Purpose

Encodes the C2A domain of synaptotagmin 1 with membrane-penetrating residue mutations M173,F234A for viral expression

Depositor

Edwin Chapman

Article

Courtney et al Nat Commun. 2019 Sep 9;10(1):4076. doi:

Insert

Syt1 (Syt1 Rat)

Use

Lentiviral

Tags

IRES-GFP

Mutation

M173, F234A

Promoter

hSyn (human synapsin I promoter)

Availability

Academic Institutions and Nonprofits only

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pBBR15.2-2H12.D11opt-scarRef

Plasmid

#221150

Purpose

cdGreen2.1 (optimized for Pseudomonas GC-content) expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11241)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2.1
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2.1)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pConRef-2H12.D11

Plasmid

#221151

Purpose

cdGreen2 expressed from a constitutive promoter in an operon with mScarlet-I (downstream) as a reference FP Internal Lab ID: 11236)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

Constitutive and None (same as cdGreen2)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pQFmcs-2H12.D11-scarREF

Plasmid

#221184

Purpose

cdGreen2 expressed from a cumate-inducible promoter in an operon with mScarlet-I (downstream) as a reference FP (Internal Lab ID: UJ11240)

Depositor

Urs Jenal

Article

Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

Inserts

cdGreen2
mScarlet-I

Expression

Bacterial

Mutation

N-terminus changed: starts with MSKKYGEAVIKE ...

Promoter

None (same as cdGreen2) and PQ5 (cumate-inducible)

Available Since

June 25, 2024

Availability

Academic Institutions and Nonprofits only

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pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

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aCat TS WT (1-697 mTFP1-TSmod-Venus 698-907)

Plasmid

#101299

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-698 and 698-906 of alpha-C…

Available Since

June 11, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-ihSyn1-DIO-tTA

Plasmid

#99121

Purpose

An AAV genome with Cre-dependent expression of the tTA from the hSyn1 promoter. Contains a positive feedback loop for amplifed expression of the tTA

Depositor

Viviana Gradinaru

Article

Chan et al Nat Neurosci. 2017 Aug;20(8):1172-1179.

Has Service

AAV1

Insert

DIO-tTA

Use

AAV

Expression

Mammalian

Mutation

*(see below)

Promoter

ihSyn1

Available Since

Sept. 15, 2017

Availability

Academic Institutions and Nonprofits only

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aCat TL1 (1-907 mTFP1-TSmod-Venus STOP)

Plasmid

#101297

Purpose

FRET-based tension sensor (TS) technology to develop a probe for molecular-level tension across alphaE-catenin.

Depositor

Alpha Yap

Article

Acharya et al Cell Rep. 2017 Mar 21;18(12):2854-2867.

Insert

alpha-Catenin

Expression

Mammalian

Mutation

Contains amino acids 1-906 of alpha-Catenin

Available Since

June 5, 2019

Availability

Academic Institutions and Nonprofits only

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Mfn2(K109A)-16xmyc

Plasmid

#26051

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

16xMyc

Expression

Mammalian

Mutation

K109A GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

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