Addgene

We narrowed to 1,188 results for: Aspa

Showing: 1021 - 1040 of 1188 results

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pLC-Flag-CSNK1A1 G40N-Puro

Plasmid

#123320

Purpose

Lentiviral vector for expression of Flag tagged CK1alpha G40N-P2A-Puro casette from a CMV promoter

Depositor

Eva Gottwein

Article

Patil et al Blood. 2018 Aug 9;132(6):577-586. doi: 1

Insert

CSNK1A1 (CSNK1A1 Human)

Use

Lentiviral

Tags

Flag

Expression

Mammalian

Mutation

changed Glycine 40 to Asparagine

Promoter

CMV

Available since

March 29, 2019

Availability

Academic Institutions and Nonprofits only

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YFP-Parkin C431N

Plasmid

#46924

Purpose

expresses mutant Parkin with YFP tag in mammalian cells

Depositor

Richard Youle

Article

Lazarou et al J Cell Biol. 2013 Jan 21;200(2):163-72.

Insert

Park2 (PRKN Human)

Use

Tags

EYFP

Expression

Mammalian

Mutation

changed cysteine 431 to asparagine

Promoter

CMV

Available since

Aug. 6, 2013

Availability

Academic Institutions and Nonprofits only

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pEBG-MKK4-S255D/T259E (GST)

Plasmid

#21566

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Aspartic Acid, Threonine 259 to Gl…

Promoter

Available since

July 17, 2009

Availability

Academic Institutions and Nonprofits only
pUNIV-SIG-5HTR4D100A

Plasmid

#16312

Depositor

Bruce Conklin

Article

Hsiao et al Proc Natl Acad Sci U S A. 2008 Jan 29. 1

Insert

h5HT4b Serotonin Receptor (Rs1 D100A RASSL) (HTR4 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

Added FLAG tag; Aspartic Acid 100 changed to Alan…

Promoter

Available since

Jan. 3, 2008

Availability

Academic Institutions and Nonprofits only
pEBG-MKK4-S255E/T259D (GST)

Plasmid

#21565

Depositor

Leonard Zon

Article

Sanchez et al Nature. . 372(6508):794-8.

Insert

mitogen-activated protein kinase kinase 4 (Map2k4 Mouse)

Use

Tags

GST

Expression

Mammalian

Mutation

Serine 255 to Glutamic Acid, Threonine 259 to As…

Promoter

Available since

July 22, 2009

Availability

Academic Institutions and Nonprofits only
human TLNRD1-F250D pET151

Plasmid

#159385

Purpose

Expresses human TLNRD1 F250D mutant in bacterial cells

Depositor

Ben Goult

Article

Cowell et al J Cell Biol. 2021 Sep 6;220(9). pii: 212

Insert

Talin Rod Domain Containing Protein 1 F250D mutant (TLNRD1 Human)

Use

Tags

His-tag, TEV cleavage site

Expression

Bacterial

Mutation

Changed Phenylalanine 250 to Aspartic Acid (F250D)

Promoter

T7

Available since

July 19, 2021

Availability

Academic Institutions and Nonprofits only

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pDEST27-GST-ATXN1 FL(92Q)-S776D

Plasmid

#21757

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 92 Q(Glutamines) and…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA3-FLAG-HA-CAD S1900A

Plasmid

#46240

Purpose

FLAG-HA-CAD with S1900 mutated to alanine

Depositor

Brendan Manning

Article

Ben-Sahra et al Science. 2013 Mar 15;339(6125):1323-8. d

Insert

carbamoyl-phosphate synthetase 2, aspartate transcarbamylase, and dihydroorotase (Cad Mouse)

Use

Tags

FLAG and HA

Expression

Mammalian

Mutation

Serine 1900 changed to Alanine (S1900A)

Promoter

CMV

Available since

Nov. 22, 2013

Availability

Academic Institutions and Nonprofits only
pDEST27-GST-ATXN1 FL(30Q)-S776D

Plasmid

#21755

Depositor

Huda Zoghbi

Article

Lim et al Nature. 2008 Apr 10. 452(7188):713-8.

Insert

Ataxin-1 (ATXN1 Human)

Use

Tags

GST

Expression

Mammalian

Mutation

insert contains a stretch of 32 amino acids (30 Q…

Promoter

CMV

Available since

July 22, 2014

Availability

Academic Institutions and Nonprofits only
pRN3P-HA-Suv4-20h1mut

Plasmid

#86690

Purpose

For transcription of of Suv4-20h1mut mRNA preceded by an HA-tag in 5'

Depositor

Maria-Elena Torres-Padilla

Article

Eid et al Genes Dev. 2016 Nov 15;30(22):2513-2526.

Insert

histone-lysine N-methyltransferase KMT5B mutant (Kmt5b Mouse)

Use

Tags

HA

Expression

Mutation

mutated region NHDC (asparagine 273 to cysteine 2…

Promoter

T3

Available since

April 26, 2017

Availability

Academic Institutions and Nonprofits only

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pGL4.23 D637G 2014 EBOV + Mucin-Like Domain

Plasmid

#86018

Purpose

Ebola Virus (Makona) Glycoprotein in CMV driven mammalian expression vector

Depositor

Pardis Sabeti, Jeremy Luban

Article

Diehl et al Cell. 2016 Nov 3;167(4):1088-1098.e6. do

Insert

Makona Ebola Virus Glycoprotein

Use

Tags

Expression

Mammalian

Mutation

Changed aspartic acid 637 to glycine

Promoter

CMV IE

Available since

Jan. 11, 2017

Availability

Academic Institutions and Nonprofits only

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Shim_AsnRS_419.110_SHI02794_TP_GFP_pK7fWG2_IVD_FP611

Plasmid

#228373

Purpose

Expresses N-term transit peptide from Sapria himalayana Asparagine aminoacyl-tRNA synthetase evm.model.fragScaff scaffold 419.110 (SHI02794); determination of S. himalayana AsnRS target localization

Depositor

Daniel Sloan

Article

DeTar et al Proc Natl Acad Sci U S A. 2024 Dec 24;12

Insert

N-terminal transit peptide from S. himalayana AsnRS evm.model.fragScaff scaffold 419.110 (SHI02794)

Use

Tags

Green Florescent Protien

Expression

Plant

Mutation

Promoter

Available since

Feb. 26, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA3-FLAG-Regnase-3_1-244 aa

Plasmid

#222659

Purpose

Mammalian expression vector to express 1-244 aa of Regnase-3 tagged with FLAG at N-term

Depositor

Silvia Monticelli

Article

Bataclan et al Life Sci Alliance. 2024 Jun 3;7(8):e2024

Insert

Zc3h12c 1-244 aa (Zc3h12c Mouse)

Use

Tags

FLAG

Expression

Mammalian

Mutation

mutated asparagine 245 to a stop codon

Promoter

CMV

Available since

Oct. 29, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PCi

Plasmid

#214038

Purpose

Bacterial expression plasmid for strep-tagged Pci from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PCi

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pJS-BCD-Strep-PC-σ

Plasmid

#214037

Purpose

Bacterial expression plasmid for strep-tagged PC-σ from Haliangium ochraceum

Depositor

Raymond Staals

Article

Steens et al Science. 2024 Feb 2;383(6682):512-519. d

Insert

PC-σ

Use

Tags

Strep-tag II

Expression

Bacterial

Mutation

WT

Promoter

T7 promoter

Available since

June 14, 2024

Availability

Academic Institutions and Nonprofits only

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pTR-455 tNGFR-P2A-WAS

Plasmid

#186082

Purpose

Knockin of truncated NGFR to target gene

Depositor

Alexander Marson

Article

Shy et al Nat Biotechnol. 2022 Aug 25:10.1038/s415

Insert

WAS (WAS Human)

Use

CRISPR

Tags

Expression

Mutation

WT with tNGFR sequence

Promoter

Available since

July 28, 2022

Availability

Academic Institutions and Nonprofits only

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pACEBac1-gamma-tubulin(N229A)-TEV-His6

Plasmid

#178077

Purpose

Insect cell expression of gamma-tubulin(N229A)-TEV-His6

Depositor

Tarun Kapoor

Article

Wieczorek et al J Cell Biol. 2021 Mar 1;220(3). pii: 211

Insert

gamma-tubulin with an N229A point mutation (TUBG1 Human)

Use

Tags

TEV-His6

Expression

Insect

Mutation

Changed Asparagine 229 to Alanine

Promoter

Available since

Feb. 4, 2022

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_G131C-N172C

Plasmid

#162586

Purpose

Expresses norovirus GI.1 VP1 protein with mutations G131C-N172C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Glycine 131 changed to Cysteine and Asparagine 17…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pFastBac1_GI.1_N167C-L169C

Plasmid

#162587

Purpose

Expresses norovirus GI.1 VP1 protein with mutations N167C-L169C in Sf9 insect cells

Depositor

Peter Kwong

Article

Verardi et al NPJ Vaccines. 2020 Dec 14;5(1):110. doi:

Insert

VP1 protein from human norovirus GI.1

Use

Tags

Expression

Insect

Mutation

Asparagine 167 changed to Cysteine and Leucine 16…

Promoter

polyhedrin

Available since

May 7, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3-HA-DEPTOR S286/287/291D

Plasmid

#159161

Purpose

Expresses DEPTOR S286/287/291D mutant in mammalian cells

Depositor

Michele Pagano

Article

Duan et al Mol Cell. 2011 Oct 21;44(2):317-24. doi:

Insert

DEPTOR (DEPTOR Human)

Use

Tags

HA

Expression

Mammalian

Mutation

changed serine 286,287 and 291 to aspartic acid

Promoter

CMV

Available since

Sept. 18, 2020

Availability

Academic Institutions and Nonprofits only

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