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plenti-UBC- IKZF4-3xHA-pGK-PUR

Plasmid

#107384

Purpose

Lentiviral expression of IKZF4-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF4 (IKZF4 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5-V5 blast

Plasmid

#83100

Purpose

Lentiviral vector for constitutive expression of human PC5A (PCSK5) with C-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pEN_TT 3xFLAG ID2

Plasmid

#98390

Purpose

Gateway entry vector for inducible expression of human ID2 with N-terminal 3xFLAG tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Entry vector for gateway cloning

Tags

3xFLAG

Promoter

TRE tight promoter

Available Since

July 21, 2017

Availability

Academic Institutions and Nonprofits only

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pIRIGF-IKZF3

Plasmid

#69046

Purpose

expression of IKZF3 fused to firefly luciferase with co-expression of Renilla luciferase, both under IRES control.

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Luciferase

Tags

luciferase

Expression

Mammalian

Promoter

IRES

Available Since

Oct. 13, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF3-3xHA-pGK-Pur

Plasmid

#69053

Purpose

lentiviral expression of IKZF3 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

May 15, 2018

Availability

Academic Institutions and Nonprofits only

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TET-pLKO.1 PURO shID2 #2

Plasmid

#83087

Purpose

Lentiviral shRNA vector for inducible knockdown of human ID2

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

shID2 (ID2 Human)

Use

Lentiviral

Expression

Mammalian

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pSLIK TT 3xFLAG ID2 hygro

Plasmid

#98394

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-UbcP-IKZF2-V2-3xHA-pGK-Pur

Plasmid

#69052

Purpose

lentiviral expression of IKZF2 variant 2 with HA tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF2 (IKZF2 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Mutation

splice variant 2

Promoter

UBC

Available Since

Oct. 17, 2017

Availability

Academic Institutions and Nonprofits only

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pSLIK TT 3xFLAG ID2 neo

Plasmid

#98393

Purpose

Lentiviral vector for inducible expression of human ID2 with 3xFLAG N-terminal tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

ID2 (ID2 Human)

Use

Lentiviral

Tags

3xFLAG

Expression

Mammalian

Available Since

July 25, 2017

Availability

Academic Institutions and Nonprofits only

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plenti-UBC- IKZF5-3xHA-pGK-PUR

Plasmid

#107385

Purpose

Lentiviral expression of IKZF5-3xHA

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF5 (IKZF5 Human)

Use

Lentiviral

Tags

HA

Expression

Mammalian

Promoter

UBC

Available Since

July 25, 2018

Availability

Academic Institutions and Nonprofits only

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pcdna3 Flag Jip3b(T266A, T276A, T287A)

Plasmid

#58924

Purpose

interacts with MAPK signaling module

Depositor

Roger Davis

Article

Kelkar et al Mol Cell Biol. 2000 Feb;20(3):1030-43.

Insert

mitogen-activated protein kinase 8 interacting protein 3 (Mapk8ip3 Mouse)

Tags

Flag

Expression

Mammalian

Mutation

changed T266A, T276A, T287A

Available Since

Sept. 10, 2014

Availability

Academic Institutions and Nonprofits only
plenti-CAG-IKZF3-Q147H-FLAG-IRES-GFP

Plasmid

#69050

Purpose

lentiviral expression of IKZF3 with Q147H mutation and FLAG tag

Depositor

William Kaelin

Article

Lu et al Science. 2014 Jan 17;343(6168):305-9. do

Insert

IKZF3 (IKZF3 Human)

Use

Lentiviral

Tags

FLAG and IRES-GFP

Expression

Mammalian

Mutation

Q147H

Promoter

CAG

Available Since

Oct. 16, 2017

Availability

Academic Institutions and Nonprofits only

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pLX304 PCSK5 (R511C)-V5 blast

Plasmid

#83104

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (R511C) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Arginine 511 to Cysteine

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (T288P)-V5 blast

Plasmid

#83101

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (A270T)-V5 blast

Plasmid

#83102

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (A270T) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Alanine 270 to Threonine

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pLX304 PCSK5 (H516P)-V5 blast

Plasmid

#83103

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (H516P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Histidine 516 to Proline

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only
pGEX-4T-1 mSesn1 (1189)

Plasmid

#66917

Purpose

bacterial expression of mouse Sesn1

Depositor

Michael Karin, Jun Hee Lee

Article

Lee et al Cell Metab. 2012 Sep 5;16(3):311-21. doi

Insert

Sesn1 (Sesn1 Mouse)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Dec. 18, 2017

Availability

Academic Institutions and Nonprofits only

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pLX302 PCSK5 (T288P)-V5 puro

Plasmid

#98709

Purpose

Lentiviral vector for constitutive expression of human mutant PC5A (T288P) with C-terminal V5 tag

Depositor

Kevin Janes

Article

Bajikar et al Dev Cell. 2017 Nov 20;43(4):418-435.e13.

Insert

PCSK5 (PCSK5 Human)

Use

Lentiviral

Tags

V5

Expression

Mammalian

Mutation

Changed Threonine 288 to Proline

Available Since

Aug. 15, 2017

Availability

Academic Institutions and Nonprofits only

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rAAV2-retro helper

Plasmid

#81070

Purpose

retrograde AAV helper vector, carrying rep and modified capsid gene

Depositor

Alla Karpova, David Schaffer

Article

Tervo et al Neuron. 2016 Oct 6. pii: S0896-6273(16)3

Inserts

rep
rAAV2-retro cap

Use

AAV and Unspecified; Helper vector

Mutation

Pro2Ala, possibly Asp613Gly and changes made to A…

Available Since

Aug. 30, 2016

Availability

Academic Institutions and Nonprofits only

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TLCV2-RB1

Plasmid

#87836

Purpose

LentiCRISPR v2 was modified into an all-in-one dox inducible system. The addition of doxycycline induces Cas9-2A-eGFP. The U6 promoter drives constitutive expression of an sgRNA targeting human RB1.

Depositor

Adam Karpf

Article

Barger et al Cancers (Basel). 2019 Feb 21;11(2). pii:

Insert

sgRB1 (RB1 Human)

Use

CRISPR and Lentiviral; Doxycycline inducible; egf…

Expression

Mammalian

Promoter

Tight TRE promoter and U6

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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