We narrowed to 4,743 results for: MLS

1. pcDNA3-Cerulean3-Cerulean3-FLARE-Cameleon

   Plasmid

   #123353

   Purpose

   Cyan-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Cerulean3-Cerulean3-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, Cerulean3, T7 tag (gene 10 leader), and Xp…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pcDNA3.1-HA-ARHGAP29

   Plasmid

   #104154

   Purpose

   mammalian expression of HA tagged ARHGAP29

   Depositor

   Marius Sudol

   Article

   Qiao et al Cell Rep. 2017 May 23;19(8):1495-1502. d

   Insert

   ARHGAP29 (ARHGAP29 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Jan. 2, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. LV-Let7d-5p

   Plasmid

   #117055

   Purpose

   Overexpression of Let-7d-5p using miR-451 backbone to enable DICER independent expression along with orange fluorescent protein (OFP) to track miRNA expression

   Depositor

   Michele De Palma

   Article

   Baer et al Nat Cell Biol. 2016 Jul;18(7):790-802. d

   Insert

   Let-7d-5p - OFP
   

   Use

   Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   SFFV promoter

   Available since

   Oct. 4, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. [pSK467] pLJC5 HA-Depdc5

   Plasmid

   #109343

   Purpose

   Lenti-viral expression of HA-tagged wildtype Depdc5

   Depositor

   Kuang Shen, David Sabatini

   Article

   Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

   Insert

   Depdc5 (DEPDC5 Human)
   

   Use

   Lentiviral

   Tags

   HA

   Expression

   Mutation

   Promoter

   Available since

   July 24, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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5. pcDNA3-Venus-Venus-FLARE-Cameleon

   Plasmid

   #123351

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-Venus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, and Xpress…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pWZLblasti-HA-ERK5

   Plasmid

   #53175

   Purpose

   Expresses HA tagged human ERK5 from blasticidin resistance retroviral vector

   Depositor

   Christopher Counter

   Article

   Brady et al Nature. 2014 Apr 9. doi: 10.1038/nature1

   Insert

   ERK5 (MAPK7 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 24, 2014

   Availability

   Academic Institutions and Nonprofits only

7. pMV361_MjTyrRS

   Plasmid

   #193250

   Purpose

   Expresses the M. jannaschii tyrosyl-tRNA synthetase (MjTyrRS)

   Depositor

   Jessica Seeliger, Peter Schultz

   Article

   Touchette et al ACS Infect Dis. 2017 May 12;3(5):336-348

   Insert

   MjTyrRS
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   hsp60

   Available since

   Feb. 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET-(-30)GFP-9xGGS-Cre-6xHis

   Plasmid

   #62372

   Purpose

   Expression of (-30)GFP-9xGGS-Cre-6xHis in bacterial cells

   Depositor

   David Liu

   Article

   Zuris et al Nat Biotechnol. 2015 Jan;33(1):73-80. do

   Insert

   (-30)GFP-9xGGS-Cre-6xHis
   

   Use

   Tags

   (-30)GFP and 6xHis tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   May 11, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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9. pLNCX2-NGN2-IRES-GFP

   Plasmid

   #90212

   Purpose

   To convert human fetal lung fibroblasts into induced cholinergic neurons (hiCN) in combination with two small molecules, forskolin and dorsomorphin.

   Depositor

   Chun-Li Zhang

   Article

   Liu et al Nat Commun. 2013;4:2183. doi: 10.1038/nc

   Insert

   NGN2-IRES-GFP (NEUROG2 Human)
   

   Use

   Retroviral

   Tags

   Expression

   Mutation

   Promoter

   CMV

   Available since

   Aug. 25, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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10. pECE M2-SNX17 WT

    Plasmid

    #69811

    Purpose

    Expresses M2-SNX17 in mammalian cells

    Depositor

    Anne Brunet

    Article

    Schaffer et al Cell Metab. 2015 Nov 3;22(5):907-21. doi

    Insert

    Sorting nexin-17 (SNX17 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    SV40 early promoter

    Available since

    May 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. [pSK468] pLJC5 Flag-Depdc5

    Plasmid

    #109337

    Purpose

    Lenti-viral expression of Flag-tagged wildtype Depdc5

    Depositor

    Kuang Shen, David Sabatini

    Article

    Shen et al Nature. 2018 Apr 5;556(7699):64-69. doi:

    Insert

    Depdc5 (DEPDC5 Human)
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mutation

    Promoter

    Available since

    July 24, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. MSCV miRNA control plasmid

    Plasmid

    #31508

    Depositor

    Chang-Zheng Chen

    Article

    Arnold et al Genome Res. 2011 May . 21(5):798-810.

    Type

    Empty backbone

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Aug. 18, 2011

    Availability

    Academic Institutions and Nonprofits only

13. pET26b-SET

    Plasmid

    #8822

    Depositor

    Judy Lieberman

    Article

    Beresford et al J Biol Chem 2001 Nov 16;276(46):43285-93

    Insert

    SET (SET Human)
    

    Use

    Tags

    6XHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 26, 2005

    Availability

    Academic Institutions and Nonprofits only

14. mCerulean3-Caveolin-C-10

    Plasmid

    #55404

    Purpose

    Localization: Membrane, Excitation: 433, Emission: 475

    Depositor

    Michael Davidson

    Article

    Markwardt et al PLoS One. 2011 . 6(3):e17896.

    Insert

    Caveolin (CAV1 Human)
    

    Use

    Tags

    mCerulean3

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

15. pRiboI-Sec-APEX2m

    Plasmid

    #176845

    Purpose

    Expression of APEX2 in the periplasm from a codon-optimized gene (single-copy integrating plasmid)

    Depositor

    Jessica Seeliger

    Article

    Ahamed et al Methods Enzymol. 2022;664:267-289. doi:

    Insert

    pRibo-Sec-APEX2m
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    pRibo, theophilline riboswitch inducible hsp60 pr…

    Available since

    Jan. 10, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-2)

    Plasmid

    #159660

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    June 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-3)

    Plasmid

    #159661

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX458-Ef1a-Cas9-H2B-mCherry (gRNA: Neurog2-1)

    Plasmid

    #159659

    Purpose

    CRISPR/Cas9 expressing plasmid containing the gRNA targeting mouse Neurog2.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Insert

    hSpCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Ef1a

    Available since

    March 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pEGFP-C1-M3(T3D)471-721

    Plasmid

    #56657

    Purpose

    GFP reporter for cytoplasmic inclusions formed by the fused, C-terminal region of protein muNS from mammalian orthoreovirus Type 3 Dearing

    Depositor

    Max Nibert

    Article

    Broering et al J Virol. 2005 May;79(10):6194-206.

    Insert

    M3(T3D)421-721
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    insert encodes aa 471-721 + native stop codon of …

    Promoter

    CMV-IE

    Available since

    June 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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20. mApple-ERM

    Plasmid

    #217377

    Purpose

    ER marker

    Depositor

    Chi-Lun Chang

    Article

    Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

    Insert

    rat cb5 ERM
    

    Use

    Tags

    mApple

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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