We narrowed to 14,764 results for: nts
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EBFP2-Cx30
Plasmid#69022PurposeExpresses human Cx30 (Gjb6 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
pAL113
Plasmid#22471PurposeExpresses N-terminal domains of PhyB (with Y276H mutation), fused to mCFP and HRas C terminal CAAX palmitoylation sequence. For red-light-induced binding to PIF6.DepositorInsertPhyB NT (PHYB Mouse, Mustard Weed)
UseSynthetic BiologyTags10aaLinker-mCFP-HrasCTExpressionMammalianMutationY276HPromoterCMVAvailable SinceNov. 5, 2009AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Kan-ADE2
Plasmid#232105PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 21, 2025AvailabilityAcademic Institutions and Nonprofits only -
MBP-MEK1
Plasmid#68300PurposeN-terminal MBP tagged human MEK1 gene for bacterial expressionDepositorAvailable SinceSept. 10, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCRISPEY-GAL-Nat-ADE2
Plasmid#232103PurposeExpresses galactose-inducible CRISPR guide RNA and repair template for creating a frameshift mutation in ADE2 gene of yeast.DepositorInsertADE2 gRNA and repair template
UseCRISPRExpressionYeastMutationRepair template introduces C at nt 466 of ADE2 to…PromoterGAL7Available SinceFeb. 20, 2025AvailabilityAcademic Institutions and Nonprofits only -
pU6-Universal.Sth.dCas9
Plasmid#171088PurposeCRISPR interference. Recipient construct for cloning 20-nt spacers into the sgRNA scaffold compatible with Streptococcus thermophilus dCas9 (CRISPR1 system) via BsaI restriction sites.DepositorInsertsToxoplasma U6 upstream region – spacer cloning site - sgRNA scaffold (compatible with S. thermophilus Cas9 CRISPR1)
DHFR-TSc3
UseToxoplasma gondii expressionMutationNote: A S245F mutation is present but did not app…PromoterDHFR-TS (Toxoplasma gondii) and U6 (Toxoplasma go…Available SinceOct. 4, 2021AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-WT-no_introns
Plasmid#131044PurposeExpresses TPI-Wildtype NMD reporter without introns and with a 21 nt hairpin in 5'UTRDepositorAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
CMVp-ECFP-Triplex-28-8xmiRNA-BS-28-pA (Construct 22)
Plasmid#55199PurposeCFP reporter along with FF4 miRNA binding sites and 28 nt sequencesDepositorInsertECFP
UseSynthetic BiologyExpressionMammalianPromoterCMVAvailable SinceJune 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
tGFP
Plasmid#26864DepositorInsertTruncated GFP-IRES-Puro
ExpressionBacterialMutationDeletion of nt 1-36Available SinceNov. 19, 2010AvailabilityAcademic Institutions and Nonprofits only -
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EBFP2-Cx43
Plasmid#69020PurposeExpresses rat Cx43 (Gja1 CDS) with an 8 amino acid linker on the N-terminus of the Connexin linking to Enhanced Blue Fluorescent Protein 2 (FP fused to NT of connexin). CMV promoter.DepositorAvailable SinceOct. 20, 2015AvailabilityAcademic Institutions and Nonprofits only -
HP21-TPI-PTC1
Plasmid#131043PurposeExpresses TPI-PTC1 NMD reporter (It has a Premature termination codon (PTC) at First Amino acid) with a 21 nt hairpin in 5'UTRDepositorAvailable SinceSept. 6, 2019AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8CQ
Plasmid#52171PurposeEncodes module 8 with amino acids 12C-16Q for recognition of nt 1ADepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12C in module 8Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-8SE
Plasmid#52172PurposeEncodes module 8 with amino acids 12S-16E for recognition of nt 1GDepositorInsertPumilio homology domain amino acids 284-319 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in module 8Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-1SE
Plasmid#52140PurposeEncodes module 1 with amino acids 12S-16E for recognition of nt 8GDepositorInsertPumilio homology domain amino acids 25-60 (PUM1 Human)
ExpressionBacterialMutationChanged 16Q to 16E in Module 1Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-2SE
Plasmid#52144PurposeEncodes module 2 with amino acids 12S-16E for recognition of nt 7GDepositorInsertPumilio homology domain amino acids 61-96 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S and 16Q to 16E in Module 2Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-3SE
Plasmid#52148PurposeEncodes module 3 with amino acids 12S-16E for recognition of nt 6GDepositorInsertPumilio homology domain amino acids 97-132 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16E in Module 3Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-4SYR
Plasmid#52158PurposeEncodes module 4 with amino acids 12S-13Y-16R for recognition of nt 5CDepositorInsertPumilio homology domain amino acids 133-168 (PUM1 Human)
ExpressionBacterialMutationChanged 12N to 12S, 13H to 13Y, and 16Q to 16R in…Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only -
pChlr-5SR
Plasmid#52162PurposeEncodes module 5 with amino acids 12S-16R for recognition of nt 4CDepositorInsertPumilio homology domain amino acids 169-204 (PUM1 Human)
ExpressionBacterialMutationChanged 12C to 12S and 16Q to 16R in Module 5Available SinceApril 28, 2014AvailabilityAcademic Institutions and Nonprofits only
