We narrowed to 11,588 results for: Vars;

1. nuc-GafD-mTurboID-HA

   Plasmid

   #184641

   Purpose

   Expresses 3xHA-tagged GlycoID construct: GafD_short-miniTurboID in mammalian nucleus for O-GlcNAc interactome tagging

   Depositor

   Charlie Fehl

   Article

   Liu et al ACS Chem Biol. 2022 Jul 12. doi: 10.1021

   Insert

   nuc-GlycoID-HA
   

   Tags

   3xHA tag and Nuclear localization sequence

   Expression

   Mammalian

   Mutation

   GafD -> expressed 22-178 ; BirA* -> express…

   Promoter

   CMV

   Available Since

   July 13, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pMSCV mSin1.1 HA

   Plasmid

   #12582

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1.1 (MAPKAP1 Human)
   

   Use

   Retroviral

   Tags

   HA

   Expression

   Mammalian

   Available Since

   Nov. 22, 2006

   Availability

   Academic Institutions and Nonprofits only

3. pMSCV mSin1.2 myc

   Plasmid

   #12577

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1.2 (MAPKAP1 Human)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-CaMKIIa-hChR2(E123T/T159C)-EYFP

   Plasmid

   #35511

   Purpose

   AAV expression of CaMKIIa-driven hChR2 variant for ultrafast optogenetic control.

   Depositor

   Karl Deisseroth

   Article

   Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

   Insert

   hChR2
   

   Use

   AAV

   Tags

   EYFP

   Expression

   Mammalian

   Mutation

   E123T and T159C

   Promoter

   CaMKIIa

   Available Since

   April 18, 2012

   Availability

   Academic Institutions and Nonprofits only

   

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5. pMSCV mSin1.5 myc

   Plasmid

   #12579

   Depositor

   David Sabatini

   Article

   Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

   Insert

   mSin1.5 (MAPKAP1 Human)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   Sept. 1, 2006

   Availability

   Academic Institutions and Nonprofits only

6. pAAV.Syn.Flex.NES-jRCaMP1b.WPRE.SV40

   Plasmid

   #100850

   Purpose

   AAV expression of Cre recombinase-activated jRCaMP1b, a red fluorescent calcium sensor protein, from the Synapsin promoter

   Depositor

   GENIE Project, Douglas Kim

   Article

   Dana et al eLife. 2016 Mar 24;5. pii: e12727. doi:

   Has Service

   AAV1

   Insert

   jRCaMP1b
   

   Use

   AAV

   Expression

   Mammalian

   Promoter

   Synapsin

   Available Since

   March 1, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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7. TMEM16F-I521K-peGFP-N1

   Plasmid

   #228588

   Purpose

   Expresses TMEM16F-I521K-peGFP in mammalian cells

   Depositor

   Huanghe Yang

   Article

   Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

   Insert

   TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   I521K plus a 3 amino acid N-terminal truncation (…

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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8. MSP2135

   Plasmid

   #72248

   Purpose

   Human expression plasmid for SpCas9-HF2 variant: CMV-T7-humanSpCas9-HF2(N497A, R661A, Q695A, Q926A, D1135E)-NLS-3xFLAG

   Depositor

   Keith Joung

   Article

   Kleinstiver et al Nature. 2016 Jan 6. doi: 10.1038/nature1

   Insert

   mammalian codon-optimized Streptococcus pyogenes Cas9 HF2(N497A/R661A/Q695A/Q926A/D1135E)-NLS-3xFlag
   

   Use

   CRISPR

   Tags

   3x FLAG and NLS

   Expression

   Mammalian

   Mutation

   N497A, R661A, Q695A, Q926A, and D1135E

   Promoter

   CMV

   Available Since

   Jan. 6, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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9. LLP453 pEf1a-NLS-aGCN4-mCherry

   Plasmid

   #100945

   Purpose

   mCherry fusion to single change variable fragment antibody GCN4 for use in SunTag system , with 3xTy1 tag

   Depositor

   Ryan Lister

   Article

   Pflueger et al Genome Res. 2018 Jun 15. pii: gr.233049.

   Insert

   scFvGCN4, sfGFP, GB1, mCherry
   

   Tags

   3xTy1

   Expression

   Mammalian

   Available Since

   June 21, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. p-spTorA-GFP-H6C

    Plasmid

    #168517

    Purpose

    GFP with the signal peptide of TorA (spTorA) in pBAD24. Includes a C-terminal HHHHHHC (6xHisC) extension.

    Depositor

    Siegfried Musser

    Article

    Bageshwar et al PLoS One. 2021 Sep 9;16(9):e0256715. doi

    Insert

    spTorA-GFP-H6C
    

    Tags

    6xHisC tag and spTorA (signal peptide of E. coli …

    Expression

    Bacterial

    Mutation

    The mut3 GFP variant with 5 additional mutations …

    Promoter

    araBAD

    Available Since

    Oct. 22, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMSCV mSin1.4 myc

    Plasmid

    #12578

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mSin1.4 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

12. Halo-Tev-Flag-Ube2v1

    Plasmid

    #110069

    Purpose

    Express Halo-Tev-Flag-Ube2v1 (human) in mammalian cells

    Depositor

    Yimon Aye

    Article

    Zhao et al ACS Cent Sci. 2018 Feb 28;4(2):246-259.

    Insert

    Halo-Tev-Flag-Ube2v1 (UBE2V1 Human)
    

    Tags

    Halo-TEV-Flag

    Expression

    Mammalian

    Promoter

    T7

    Available Since

    July 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. TMEM16F-I611K-peGFP-N1

    Plasmid

    #228581

    Purpose

    Expresses mouse TMEM16F-I611K-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    TMEM16F/anoctamin 6 (Ano6), transcript variant 2 (Ano6 Mouse)
    

    Expression

    Mammalian

    Mutation

    I611K plus a 3 amino acid N-terminal truncation (…

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pMSCV mSin1.2 HA

    Plasmid

    #12583

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mSin1.2 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    HA

    Expression

    Mammalian

    Available Since

    Nov. 30, 2006

    Availability

    Academic Institutions and Nonprofits only

15. pMSCV mSin1.1 myc

    Plasmid

    #12576

    Depositor

    David Sabatini

    Article

    Frias et al Curr Biol. 2006 Sep 19;16(18):1865-70. d

    Insert

    mSin1.1 (MAPKAP1 Human)
    

    Use

    Retroviral

    Tags

    myc

    Expression

    Mammalian

    Available Since

    Sept. 1, 2006

    Availability

    Academic Institutions and Nonprofits only

16. pAAV-Syn-pAceR-Kv2.1PR

    Plasmid

    #195525

    Purpose

    Red fluorescent, positive response-polarity voltage indicator under the control of synapsin promoter; soma-targeted

    Depositor

    Vincent Pieribone

    Article

    Kannan et al Science. 2022 Nov 4;378(6619):eabm8797.

    Insert

    pAceR-Kv2.1 proximal restriction sequence
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    VARNAM 78E, 81D, 92N; SI linker

    Promoter

    Synapsin

    Available Since

    Jan. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAV-TRE-flex-Clover

    Plasmid

    #135177

    Purpose

    AAV expression of TRE(TRE3G)-driven, Cre-dependent, Clover expression for fluorescent labling

    Depositor

    Tetsuo Yamamori

    Article

    Ebina et al Proc Natl Acad Sci U S A. 2019 Nov 5;116

    Inserts

    Clover
    TRE3G
    

    Use

    AAV

    Expression

    Mammalian

    Mutation

    a variant of GFP

    Promoter

    TRE3G

    Available Since

    Jan. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pTH732-2µ-RLuc/staCFLuc

    Plasmid

    #40607

    Depositor

    Tobias von der Haar

    Article

    Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

    Inserts

    Firefly Luciferase
    Renilla luciferase
    

    Expression

    Yeast

    Mutation

    The last three codons of the full-length Firefly …

    Promoter

    ADH1 and TDH3

    Available Since

    Oct. 30, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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19. pAAV-CaMKIIa-hChR2(E123A)-mCherry

    Plasmid

    #35506

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Insert

    hChR2
    

    Use

    AAV

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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20. pAAV-CaMKIIa-hChR2(E123A)-EYFP

    Plasmid

    #35505

    Purpose

    AAV expression of CaMKIIa-driven hChR2 variant CheTA 2.0 for ultrafast optogenetic control.

    Depositor

    Karl Deisseroth

    Article

    Mattis et al Nat Methods. 2011 Dec 18;9(2):159-72. do

    Has Service

    AAV9

    Insert

    hChR2
    

    Use

    AAV

    Tags

    EYFP

    Expression

    Mammalian

    Mutation

    E123A

    Promoter

    CaMKIIa

    Available Since

    April 18, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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