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EF1a_KLF16_P2A_Hygro_Barcode

Plasmid

#120502

Purpose

Barcoded lentiviral vector to express KLF16 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF16 (KLF16 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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EF1a_MYOG_P2A_Hygro_Barcode

Plasmid

#120465

Purpose

Barcoded lentiviral vector to express MYOG in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

MYOG (MYOG Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 5, 2019

Availability

Academic Institutions and Nonprofits only

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pQuantA-hygro-amp

Plasmid

#45584

Depositor

Andrzej Dziembowski

Article

Orlowska et al Nucleic Acids Res. 2013 Sep;41(17):e167.

Type

Empty backbone

Use

Cre/Lox; Dt40 cell line targeting

Tags

Quant-A tag

Available Since

July 8, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynapFLEX.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244099

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 23, 2025

Availability

Academic Institutions and Nonprofits only

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pAAV.GFAP.(cyto).iGlucoSnFR2.mRuby3

Plasmid

#244084

Purpose

Cytosolic expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

mRuby3

Available Since

Sept. 16, 2025

Availability

Academic Institutions and Nonprofits only

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pMOS031: superecliptic pHluorin (endoplasmic reticulum)

Plasmid

#163051

Purpose

superecliptic pHluorin (endoplasmic reticulum)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

superecliptic pHluorin

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pMIII-Neo-BFP

Plasmid

#153426

Purpose

Retroviral (MSCV) expression of mTagBFP2 fluorescent protein

Depositor

Maki Nakayama

Article

Mann et al Front Immunol. 2020 Apr 9;11:633. doi: 1

Insert

mTagBFP2

Use

Retroviral

Expression

Mammalian

Available Since

Aug. 11, 2020

Availability

Industry, Academic Institutions, and Nonprofits

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peGFP-N1-Cofilin

Plasmid

#186747

Purpose

Expresses wildtype GFP-tagged human cofilin in mammalian cells

Depositor

Geert van den Bogaart

Article

Warner et al Cell Rep. 2024 Feb 27;43(3):113866. doi:

Insert

Cofilin-1 (CFL1 Human)

Tags

GFP

Expression

Mammalian

Available Since

March 4, 2024

Availability

Academic Institutions and Nonprofits only

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EF1a_KLF10_P2A_Hygro_Barcode

Plasmid

#120496

Purpose

Barcoded lentiviral vector to express KLF10 in mammalian cells under control of EF1a promoter. Barcoded for transcript detection in single cell RNA-seq.

Depositor

Prashant Mali

Article

Parekh et al Cell Syst. 2018 Nov 12. pii: S2405-4712(

Insert

KLF10 (KLF10 Human)

Use

Lentiviral

Promoter

EF1a

Available Since

Feb. 6, 2019

Availability

Academic Institutions and Nonprofits only

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6xHis-TEV-UBC-9

Plasmid

#87055

Purpose

Expresses 6xHis-tagged ubc-9, with a TEV protease cleavage site between the tag and the ORF

Depositor

Ronald Hay

Article

Pelisch et al Nat Commun. 2014 Dec 5;5:5485. doi: 10.1

Insert

UBC-9 (ubc-9 Nematode)

Tags

6xHis-TEV

Expression

Bacterial

Promoter

T7

Available Since

April 3, 2017

Availability

Academic Institutions and Nonprofits only

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pGALT-EGFP-IRES-puro2b

Plasmid

#26463

Depositor

Daniel Gerlich

Article

Held et al Nat Methods. 2010 Sep . 7(9):747-54.

Insert

pGalT

Tags

EGFP

Expression

Bacterial and Mammalian

Available Since

Dec. 1, 2010

Availability

Academic Institutions and Nonprofits only

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pBEAST-J23101-CocE

Plasmid

#114600

Purpose

"Strong constitutive expression of metabolic enzyme, CocE, for cell-free expression"

Depositor

Jerome Bonnet

Article

Voyvodic et al Nat Commun. 2019 Apr 12;10(1):1697. doi:

Insert

CocE

Use

Synthetic Biology; Cell-free protein synthesis

Promoter

J23101

Available Since

April 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLXSN p110 CUX1

Plasmid

#90471

Purpose

Retroviral vector expressing human p110 CUX1 (amino acids 747-1505) with a Myc and HA tag at the N- and C-terminue, respectively

Depositor

Alain Nepveu

Article

Kedinger et al J Biol Chem. 2009 Oct 2;284(40):27701-11

Insert

CUX1 (amino acids 747-1505) (CUX1 Human)

Use

Retroviral

Tags

HA and Myc

Expression

Mammalian

Promoter

Moloney murine leukemia virus long terminal repeat

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pAAV.hSynap.(mem).iGlucoSnFR2.mRuby3

Plasmid

#244097

Purpose

Plasma membrane targeted expression of green glucose sensor with non-responsive mRuby3

Depositor

Tim Brown, HHMI-JRC Tool Translation Team

Article

Marvin et al Sci Adv. 2025 Nov 14;11(46):eadz3889. do

Insert

iGlucoSnFR2.mRuby3

Use

AAV

Tags

IgG kappa leader and mRuby3

Available Since

Dec. 2, 2025

Availability

Academic Institutions and Nonprofits only

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APEX2-SOPP3-TM

Plasmid

#229077

Purpose

Expresses APEX2-SOPP3 in the cell surface

Depositor

Weijun Pan

Article

Qu et al Cell Res. 2025 Feb;35(2):149-152. doi: 1

Insert

Express APEX2-SOPP3 in the cell surface (APEX2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 5, 2025

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM4-Halo-FRB

Plasmid

#197057

Purpose

Expression of Paraburkholderia xenovorans encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_GEM7-Halo-FRB

Plasmid

#197058

Purpose

Expression of Rhodospirillum rubrum encapsulin Halo-FRB-fusion in mammalian cells

Depositor

Julia Mahamid

Article

Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.

Insert

Encapsulin

Tags

FRB and HaloTag

Expression

Mammalian

Mutation

Codon-optimised for human cell expression

Available Since

Sept. 20, 2023

Availability

Academic Institutions and Nonprofits only

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pMOS009E: Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Plasmid

#163062

Purpose

Entry vector for: QuasAr2-mOrange2 voltage sensor (plasma membrane)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

QuasAr2

Use

Gateway Cloning

Tags

mOrange2

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pQE80L TriCatcher-RGDSP

Plasmid

#112631

Purpose

Two SpyCatchers linked by an elastin-like polypeptide with an RGDSP integrin-binding site and a C-terminal SnoopCatcher

Depositor

Mark Howarth

Article

Wieduwild et al Biomaterials. 2018 Oct;180:253-264. doi:

Insert

TriCatcher-RGDSP

Tags

His6 and TEV cleavage site

Expression

Bacterial

Mutation

central RGDSP integrin-binding sequence

Promoter

T5

Available Since

Sept. 7, 2018

Availability

Academic Institutions and Nonprofits only

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Rix-PGK-Tom-W

Plasmid

#25813

Depositor

Jozsef Kiss, Patrick Salmon

Article

Dayer et al Brain. 2007 Nov . 130(Pt 11):2962-76.

Insert

Rix-PGK-Tom-W

Use

Lentiviral

Mutation

tdTomato from Tsien lab

Available Since

June 9, 2011

Availability

Academic Institutions and Nonprofits only

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