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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 31,699 results for: multi

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Showing: 10381 - 10400 of 31699 results

  17. pDG459

    Plasmid
    #100901
    Purpose
    SpCas9 with 2A-Puro and a cloning backbone for 2 custom gRNAs which can be cloned in via a one-step reaction. For generation of double knock-outs and large deletions in a single plasmid system.
    Depositor
    Paul Thomas
    Article
    Adikusuma et al PLoS One. 2017 Dec 6;12(12):e0187236. do
    Inserts
    humanized CRISPR associated protein 9
    U6-gRNA scaffold 1
    U6-gRNA scaffold 2
    Use
    CRISPR and Mouse Targeting
    Tags
    3xFLAG and T2A-PuroR
    Expression
    Mammalian
    Promoter
    CBh and U6
    Available Since
    Dec. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100901-plasmid-map-sequence-id-197197

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  18. PM-FRB-CFP

    Plasmid
    #67517
    Purpose
    FRB conjugated plasma membrane targeting sequences from GAP43 (MLCCMRRTKQVEKNDDQKI)
    Depositor
    Tamas Balla
    Article
    Varnai et al J Cell Biol. 2006 Nov 6;175(3):377-82.
    Insert
    mTOR (MTOR Human)
    Tags
    CFP, HA, and PM tethering sequence (MLCCMRRTKQVEK…
    Expression
    Mammalian
    Mutation
    FRB domain only (residues 2019–2114)
    Promoter
    CMV
    Available Since
    Aug. 25, 2015
    Availability
    Academic Institutions and Nonprofits only
Showing: 10381 - 10400 of 31699 results