We narrowed to 13,693 results for: crispr cas9

1. pHDR-eGFP (pXK-1647)

   Plasmid

   #208824

   Purpose

   The HDR-based surrogate reporter for validating HDR-editing activity of different gene editing tools as well as for helping to screen HDR-editing positive cells. Key Construct: CAG-PuroR-CMV-eGFP*(HDR

   Depositor

   Kun Xu

   Article

   Shao et al Int J Biochem Cell Biol. 2017 Nov;92:43-

   Type

   Empty backbone

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Dec. 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. ScFv-2ERT2-V

   Plasmid

   #120553

   Purpose

   3rd gen transfer vector. Encode an antibody that binds to the GCN4 peptide from the SunTag system, and is fused to sfGFP, 2 tandem ERT2 and VP64.

   Depositor

   Yu Wang

   Article

   Lu et al Nucleic Acids Res. 2018 Mar 16;46(5):e25

   Insert

   scFvGCN4, sfGFP, GB1, ERT2, VP64
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   Aug. 22, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. AAV-tLuc

   Plasmid

   #107552

   Purpose

   tLuc in AAV backbone

   Depositor

   Juan Belmonte

   Article

   Liao et al Cell. 2017 Dec 14;171(7):1495-1507.e15.

   Insert

   Luciferase
   

   Use

   AAV

   Available Since

   July 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. pGG426_UV5

   Plasmid

   #165608

   Purpose

   Vector for expression of the SpCas9 VRKG variant with sgRNA in E. coli: lacUV5-VRKG(SpCas9, D1135V/S1136R/D1332K/R1333G) and UV5-sgRNA (hEGFP spacer)

   Depositor

   Marcus Noyes

   Article

   Goldberg et al Nat Commun. 2021 Jan 13;12(1):349. doi:

   Insert

   lacUV5 driving Streptococcus pyogenes Cas9 VRKG(D1135V/S1136R/D1332K/R1333G) and hEGFP-sgRNA
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Bacterial

   Mutation

   D1135V, S1136R, D1332K and R1333G mutations in Sp…

   Promoter

   lacUV5 driving Cas9 VRKG and UV5 driving sgRNA

   Available Since

   April 21, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. Tol2-LyzC-RacFRET-U6ac-rac2 guides

   Plasmid

   #168248

   Purpose

   "label active Rac under Rac2 neutrophil-specific knockout background"

   Depositor

   Qing Deng

   Article

   Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

   Insert

   RacFRET
   

   Use

   Zebrafish expression

   Promoter

   LyzC

   Available Since

   March 16, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. tRNA-gRNA position [n] (GB1207)

   Plasmid

   #75408

   Purpose

   tRNA and scaffold for the assembly of GBoligomers for the last position (positon [n]) of a polycistronic tRNA-gRNA (2- and 3-part multiplexing)

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   tRNA-gRNA position [n]
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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7. pU6-1 (GB1204)

   Plasmid

   #75405

   Purpose

   Provides the A. thaliana U6-1 RNA polIII promoter as a level 0 GoldenBraid part

   Depositor

   Diego Orzaez

   Article

   Vazquez-Vilar et al Plant Methods. 2016 Feb 1;12:10. doi: 10

   Insert

   AtU6-1 promoter
   

   Use

   CRISPR and Synthetic Biology

   Expression

   Plant

   Available Since

   Aug. 5, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330_NPM2_cterm1

   Plasmid

   #222911

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 1 (NPM2 Synthetic, Human)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. pX330_NPM2_cterm4

   Plasmid

   #222914

   Purpose

   Cas9/sgRNA plasmid for targeting NPM2

   Depositor

   George Church

   Article

   Pierson Smela et al bioRxiv 2022.07.11.499564

   Insert

   Cas9, NPM2 sgRNA 4 (NPM2 Synthetic, Human)
   

   Use

   CRISPR

   Available Since

   July 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pX330_NPM2_cterm3

    Plasmid

    #222913

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 3 (NPM2 Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX330_NPM2_cterm2

    Plasmid

    #222912

    Purpose

    Cas9/sgRNA plasmid for targeting NPM2

    Depositor

    George Church

    Article

    Pierson Smela et al bioRxiv 2022.07.11.499564

    Insert

    Cas9, NPM2 sgRNA 2 (NPM2 Synthetic, Human)
    

    Use

    CRISPR

    Available Since

    July 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. Tol2-LyzC-PBD-GFP-U6ac-rac2 guides

    Plasmid

    #168251

    Purpose

    "label active Rac under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    PBD GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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13. Tol2-LyzC-Utrophin-GFP-U6ac-Rac2 guides

    Plasmid

    #168254

    Purpose

    "label stable F-actin under Rac2 neutrophil-specific knockout background"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    Oct. 3, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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14. Tol2-LyzC-PBD-GFP-U6ac-ctrl guides

    Plasmid

    #168252

    Purpose

    "label active Rac; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    PBD GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. Tol2-LyzC-Utrophin-GFP-U6ac-ctrl guides

    Plasmid

    #168253

    Purpose

    "label stable F-actin; control for neutrophil-specific knockout"

    Depositor

    Qing Deng

    Article

    Wang et al J Cell Sci. 2021 Apr 15;134(8). pii: 237

    Insert

    Utrophin-GFP
    

    Use

    Zebrafish expression

    Promoter

    LyzC

    Available Since

    March 16, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRH2502

    Plasmid

    #84379

    Purpose

    Expression of dcas9 D10A H840A from a TetR-regulated uvtetO promoter

    Depositor

    Robert Husson

    Article

    Singh et al Nucleic Acids Res. 2016 Jul 12. pii: gkw

    Insert

    dcas9
    

    Use

    CRISPR

    Expression

    Bacterial

    Mutation

    Aspartate at position 10 changed to Alanine, Hist…

    Promoter

    uv15tetO

    Available Since

    Feb. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. PX458_NFIA_iso1_2

    Plasmid

    #104049

    Purpose

    Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 gRNA (NFIA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. PX458_NFIA_iso1_1

    Plasmid

    #104048

    Purpose

    Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    NFIA_iso1 gRNA (NFIA Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. PX458_RXRB_1

    Plasmid

    #104050

    Purpose

    Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RXRB gRNA (RXRB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. PX458_RXRB_2

    Plasmid

    #104051

    Purpose

    Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP

    Depositor

    Eric Mendenhall, Richard M. Myers

    Article

    Savic et al Genome Res. 2015 Sep 9.

    Insert

    RXRB gRNA (RXRB Human)
    

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    Dec. 8, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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