We narrowed to 61,283 results for: sap

1. GCH1_Halo_N_allele

   Plasmid

   #178159

   Purpose

   Donor vector for endogenous tagging of human GCH1 at the N-terminus with halotag

   Depositor

   iPSC Neurodegenerative Disease Initiative (iNDI), Michael Ward

   Insert

   Halotag (GCH1 Human)
   

   Use

   Donor vector

   Available Since

   Dec. 2, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. pH6HTC_PKM-Halo

   Plasmid

   #175321

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   PKM (PKM Human)
   

   Use

   Cell free expression

   Tags

   HaloTag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pH6HTN_Halo-STMN1

   Plasmid

   #175299

   Purpose

   Production of HaloTag fusion protein

   Depositor

   Claudia Kutter

   Article

   Atanasoai et al bioRxiv 2021.11.08.467743

   Insert

   STMN1 (STMN1 Human)
   

   Use

   Cell free expression

   Tags

   6xHis-HaloTag

   Expression

   Bacterial

   Available Since

   Oct. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. Epac-S-H127

   Plasmid

   #170343

   Purpose

   6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Insert

   6xHis_mT2Del_EPACdDEPCD_cp173Ven(ST)_Ven(ST) (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   CMV

   Available Since

   Oct. 5, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. TetO::EGFP-C-Meikin

   Plasmid

   #174706

   Purpose

   Doxycycline inducible expression of human C-Meikin Separase cleavage fragment

   Depositor

   Iain Cheeseman

   Article

   Maier et al Dev Cell. 2021 Aug 9;56(15):2192-2206.e8

   Insert

   Meikin
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   155-373

   Available Since

   Sept. 22, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. p663-UBC-miniTurbo-V5-RSKR_IDG-K

   Plasmid

   #170607

   Purpose

   Gateway destination clone of RSKR (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-RSKR (SGK494 Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Sept. 1, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. p663-UBC-miniTurbo-V5-PDIK1L_IDG-K

   Plasmid

   #170596

   Purpose

   Gateway destination clone of PDIK1L (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

   Depositor

   Ben Major

   Insert

   miniTurbo-V5-PDIK1L (PDIK1L Human)
   

   Use

   Lentiviral; Gateway destination

   Tags

   miniTurbo-V5

   Expression

   Mammalian

   Promoter

   Ubiquitin

   Available Since

   Aug. 12, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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8. Epac-S-H151

   Plasmid

   #170346

   Purpose

   6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.

   Depositor

   Kees Jalink

   Insert

   6xHis_mT2Del_EPACdDEPCD_Q270E_cp174Cit (RAPGEF3 Human)
   

   Expression

   Mammalian

   Mutation

   Deletion of DEP domain, Catalytically dead T781A …

   Promoter

   SP6

   Available Since

   Aug. 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. SPELL-GDI Component 2

   Plasmid

   #170487

   Purpose

   SPELL GDI1 C-lobe fused to FRB. Split site is 66

   Depositor

   Klaus Hahn

   Article

   Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

   Insert

   FRB-GDI1(C-lobe)
   

   Expression

   Mammalian

   Available Since

   July 27, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. SPELL-GDI Component 1

    Plasmid

    #170486

    Purpose

    SPELL GDI1 N-lobe fused to iFKBP. Split site is 66.

    Depositor

    Klaus Hahn

    Article

    Dagliyan et al Nat Commun. 2018 Oct 2;9(1):4042. doi: 1

    Insert

    GDI1(N-lobe)-iFKBP
    

    Expression

    Mammalian

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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11. pKB237.1

    Plasmid

    #170031

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL19 fragment lacking alpha-helix sequence and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL19 Δ alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 6, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pKB235.2

    Plasmid

    #170025

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL20 alpha-helix fragment and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL20 alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pKB237.2

    Plasmid

    #170032

    Purpose

    Encodes LacI-controlled expression of surface-displayed CCL19 alpha-helix fragment and PvirK-mNeonGreen reporter

    Depositor

    Jeffrey Tabor

    Article

    Brink et al bioRxiv

    Inserts

    mNeonGreen
    CCL19 alpha-helix
    

    Use

    Synthetic Biology

    Tags

    Lpp, OmpA, and Tether

    Expression

    Bacterial

    Promoter

    Ptac and PvirK

    Available Since

    July 1, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCIneo-AR-S650A

    Plasmid

    #171232

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser650Ala (AR-S650A)

    Depositor

    Steven Balk

    Insert

    human androgen receptor Ser650Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser650Ala (AR-S650A)

    Promoter

    CMV

    Available Since

    June 30, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCIneo-AR-S81A

    Plasmid

    #171223

    Purpose

    Mammalian expression of un-tagged human androgen receptor phosphorylation mutant: AR-Ser81Ala (AR-S81A)

    Depositor

    Steven Balk

    Insert

    human androgen receptor Ser81Ala mutant (AR Human)
    

    Expression

    Bacterial and Mammalian

    Mutation

    AR-Ser81Ala (AR-S81A)

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. p663-UBC-miniTurbo-V5-TSSK6_IDG-K

    Plasmid

    #170618

    Purpose

    Gateway destination clone of TSSK6 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TSSK6 (TSSK6 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. p663-UBC-miniTurbo-V5-TSSK3_IDG-K

    Plasmid

    #170617

    Purpose

    Gateway destination clone of TSSK3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TSSK3 (TSSK3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. p663-UBC-miniTurbo-V5-TSSK1B_IDG-K

    Plasmid

    #170616

    Purpose

    Gateway destination clone of TSSK1B (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-TSSK1B (TSSK1B Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. p663-UBC-miniTurbo-V5-STK40_IDG-K

    Plasmid

    #170613

    Purpose

    Gateway destination clone of STK40 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-STK40 (STK40 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. p663-UBC-miniTurbo-V5-SCYL3_IDG-K

    Plasmid

    #170606

    Purpose

    Gateway destination clone of SCYL3 (human) tagged with N-terminal miniTurbo-V5 for generating protein-proximity networks using proximity-dependent biotinylation proteomics

    Depositor

    Ben Major

    Insert

    miniTurbo-V5-SCYL3 (SCYL3 Human)
    

    Use

    Lentiviral; Gateway destination

    Tags

    miniTurbo-V5

    Expression

    Mammalian

    Promoter

    Ubiquitin

    Available Since

    June 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge