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373 pTol2-fli1ep:CherryDest

Plasmid

#73493

Purpose

Tol2 vector with fli1ep element upstream of mCherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:mCherry-attR1-attR2

Available Since

March 7, 2016

Availability

Academic Institutions and Nonprofits only

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pX52

Plasmid

#169444

Purpose

Used for guide cloning with PCR

Depositor

Richard Huganir

Article

Fang et al Elife. 2021 Mar 10;10. pii: 65202. doi:

Type

Empty backbone

Use

CRISPR

Available Since

June 11, 2021

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-sfGFP

Plasmid

#49645

Purpose

TALEGreen-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

sfGFP

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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pToneTP

Plasmid

#67692

Purpose

For in vitro transcription of mRNA encoding Tol1 Transposase

Depositor

Nathan Lawson

Article

Shin et al Development. 2016 Oct 15;143(20):3785-37

Insert

Tol1 transposase

Available Since

July 31, 2015

Availability

Academic Institutions and Nonprofits only

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pJPC13

Plasmid

#80773

Purpose

Cloning vector compatible with ColE1 plasmids with medium copy number

Depositor

Gregory Phillips

Article

Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

May 11, 2018

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mCherry

Plasmid

#49647

Purpose

TALERed-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mCherry

Promoter

T7

Available Since

Jan. 3, 2014

Availability

Academic Institutions and Nonprofits only

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370 pTol2-lmo2:CherryDest

Plasmid

#73489

Purpose

Tol2 vector with lmo2 promoter upstream of Cherry, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

lmo2 promoter:mCherry-attR1-attR2

Available Since

March 14, 2016

Availability

Academic Institutions and Nonprofits only

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pWarf(+)

Plasmid

#34562

Depositor

Jeff Abramson

Article

Hsieh et al Protein Sci. 2010 Apr;19(4):868-80.

Insert

HRV 3C-GPA-GFP-8HIS

Expression

Bacterial

Available Since

Feb. 23, 2012

Availability

Academic Institutions and Nonprofits only

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pTHSSe_1

Plasmid

#109233

Purpose

Empty AmpR pSC101 vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Type

Empty backbone

Expression

Bacterial

Available Since

July 23, 2018

Availability

Academic Institutions and Nonprofits only

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pJPK13

Plasmid

#80775

Purpose

Cloning vector compatible with ColE1 plasmids with high copy number

Depositor

Gregory Phillips

Article

Peterson et al Plasmid. 2008 May;59(3):193-201. doi: 10

Type

Empty backbone

Expression

Bacterial

Available Since

Nov. 2, 2017

Availability

Academic Institutions and Nonprofits only

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pET22b-EC12WTC9AAvi

Plasmid

#47925

Purpose

Expresses E-cadherin EC12 WT with C9A mutation and avi tag in E.Coli.

Depositor

Tanja Kortemme

Article

Ritterson et al J Am Chem Soc. 2013 Aug 7.

Insert

E-Cadherin EC12

Tags

6xHis, Avi Tag, and TEV Cleavage

Expression

Bacterial

Mutation

changed Cysteine 9 to Alanine

Promoter

T7

Available Since

Sept. 27, 2013

Availability

Academic Institutions and Nonprofits only

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371 pTol2-fli1ep:MTDest

Plasmid

#73490

Purpose

Tol2 vector with fli1ep element upstream of 6xmyc, attR1/R2 cassette, and late SV40 pA signal

Depositor

Nathan Lawson

Article

Villefranc et al Dev Dyn. 2007 Nov . 236(11):3077-87.

Insert

fli1ep:6xMyc-attR1-attR2

Available Since

April 12, 2017

Availability

Academic Institutions and Nonprofits only

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Endophilin SH3 domains

Plasmid

#47401

Depositor

Peter McPherson

Article

Micheva et al J Biol Chem. 1997 Oct 24;272(43):27239-4

Insert

Endophilin

Tags

GST

Expression

Bacterial

Mutation

SH3 domain (aa 292-352)

Promoter

tac

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only
pAM1518

Plasmid

#40238

Depositor

Susan Golden

Article

Andersson et al Methods Enzymol. 2000;305:527-42.

Insert

psbAI promoter, luxCDE

Use

Cyanobacteria cloning vector

Promoter

n/a

Available Since

March 25, 2014

Availability

Academic Institutions and Nonprofits only
LT1_30_backbone_X2_integration

Plasmid

#194403

Purpose

Multi Backbone with X2 homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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LT1_31_backbone_HO_integration

Plasmid

#194404

Purpose

Multi Backbone with HO homology arms for Saccharomyces cerevisiae

Depositor

Lisbeth Olsson

Article

Torello Pianale et al ACS Synth Biol. 2023 Aug 8. doi: 10.1021

Type

Empty backbone

Use

Synthetic Biology

Expression

Bacterial

Mutation

NA

Available Since

May 8, 2023

Availability

Academic Institutions and Nonprofits only

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pBlueScript NCX1.1

Plasmid

#66799

Depositor

Michela Ottolia, Kenneth Philipson

Article

Nicoll et al Science. 1990 Oct 26;250(4980):562-5.

Insert

NCX1.1

Use

T3 transcription of gene

Mutation

there are 3 silent mutations within the open read…

Promoter

T3

Available Since

Aug. 7, 2015

Availability

Academic Institutions and Nonprofits only

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pT7CFE1-TelR15-mTagBFP2

Plasmid

#49643

Purpose

TALEBlue-Telomere for In Vitro Translation

Depositor

Thoru Pederson

Article

Ma et al Proc Natl Acad Sci U S A. 2013 Dec 9.

Insert

TelR15

Use

In vitro translation

Tags

mTagBFP2

Promoter

T7

Available Since

April 3, 2015

Availability

Academic Institutions and Nonprofits only

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pTHSSe_2

Plasmid

#109234

Purpose

Empty SpecR p15A vector

Depositor

Christopher Voigt

Article

Segall-Shapiro et al Nat Biotechnol. 2018 Mar 19. pii: nbt.41

Type

Empty backbone

Expression

Bacterial

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNAFlagMenin(S394A, T397A, S399A)

Plasmid

#32081

Depositor

Matthew Meyerson

Article

Francis et al PLoS One. 2011 . 6(1):e16119.

Insert

Menin

Tags

Flag

Expression

Mammalian

Mutation

S394A,T397A, S399A

Promoter

CMV

Available Since

Sept. 19, 2011

Availability

Academic Institutions and Nonprofits only