We narrowed to 46,864 results for: cha

Showing: 10441 - 10460 of 46864 results

Results per page:

Sort results by:

MP28444 – pFUSE2ss-aRD114_CHIg-mIgG2A

Plasmid

#110555

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable heavy chain of anti-RD114 binder fused to murine IgG2A constant heavy chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

Anti-RD114 heavy chain

Tags

mIgG2A

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
sgTrack-BFP

Plasmid

#164273

Purpose

Empty sgRNA expression vector with co-expression of BFP reporter

Depositor

Charles Rudin

Article

Wohlhieter et al Cell Rep. 2020 Dec 1;33(9):108444. doi:

Type

Empty backbone

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6 and EFS

Available Since

Feb. 3, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
MBP-TSF-TEVcs-ULK1

Plasmid

#171416

Purpose

For the purifcation of human ULK1 complex

Depositor

James Hurley

Article

Chang et al Sci Adv. 2021 Apr 23;7(17). pii: 7/17/ea

Insert

ULK1 (ULK1 Synthetic)

Expression

Mammalian

Available Since

July 13, 2021

Availability

Academic Institutions and Nonprofits only

Enlarge
pFsynW SYP-GFP

Plasmid

#197283

Purpose

Encodes for viral expression of full-length wild type synaptophysin (SYP). Visualized by a C-terminal GFP.

Depositor

Edwin Chapman

Article

Watson et al Elife. 2023 Feb 2;12:e82568. doi: 10.755

Insert

SYP and GFP (Syp Rat)

Use

Lentiviral

Tags

1D4 epitope

Mutation

N/A

Promoter

hSyn (human synapsin I promoter)

Available Since

July 28, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
pRR-ERalpha-5Z

Plasmid

#23061

Depositor

Charles Miller

Article

Miller CA et al Plasmid. 2010 Mar;63(2):73-8. doi: 10.10

Insert

ERalpha (ESR1 Human)

Expression

Yeast

Available Since

March 31, 2010

Availability

Academic Institutions and Nonprofits only
Halotag-6xHp

Plasmid

#214414

Purpose

Lipid droplet localization of 6xHp of Spastin fused to Halotag

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

6x Spastin aa 43-92 (SPAST Human)

Tags

Halo

Expression

Mammalian

Promoter

CMV

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
strep-IRF8

Plasmid

#102872

Purpose

Express strep-tagged mouse IRF8 protein in E. coli

Depositor

Gary Stormo

Article

Chang et al BMC Mol Biol. 2018 Mar 27;19(1):5. doi:

Insert

IRF8 (Irf8 Mouse)

Tags

strep tag

Expression

Bacterial

Promoter

T7

Available Since

April 5, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaG94-5

Plasmid

#228965

Purpose

For bacterial expression of anti-GFP nanobody LaG94-5, with pelB leader for periplasmic secretion, and C-term free cysteine and 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaG94-5

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
pCAIP-hXRCC1pd-eGFP

Plasmid

#206033

Purpose

Mammalian expression of PAR-deficient hXRCC1pd coupled to eGFP under the control of a CAG promoter

Depositor

Primo Schaer

Article

Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

Insert

hXRCC1

Tags

eGFP

Expression

Mammalian

Mutation

S103A,R186A,S184A,S193A,S219A,S220A,S236A,S268A

Promoter

CAG

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pMax_mOrange_A215C

Plasmid

#177828

Purpose

Site-specific mutagenesis of mOrange for the purposes of creating a site-specific 8-oxo-G:C lesion for the purposes of measuring OGG1 activity via Host Cell Reactivation (HCR).

Depositor

Zachary Nagel, Leona Samson

Article

Nagel et al Proc Natl Acad Sci U S A. 2014 May 6;111

Insert

mOrange_A215C

Expression

Mammalian

Mutation

A215C

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

Enlarge
MP28445 – pFUSE2ss-aRD114_CLIg-mIgK

Plasmid

#110556

Purpose

pFUSE backbone with an IL2 signal sequence, expressing the variable light chain of anti-RD114 binder fused to murine Kappa constant light chain.

Depositor

Martin Pule

Article

Mekkaoui et al J Gen Virol. 2019 Nov 8. doi: 10.1099/jg

Insert

anit-RD114 light chain

Tags

mKappa

Expression

Bacterial and Mammalian

Promoter

EF-1 alpha core promoter

Available Since

Aug. 1, 2019

Availability

Academic Institutions and Nonprofits only

Enlarge
pFUW PRKN-P2A-PuroR

Plasmid

#239571

Purpose

Overexpression of human PARK2

Depositor

Tim Ahfeldt, Joel Blanchard

Article

et al iScience 28 (2025)

Insert

PRKN (PRKN Human)

Use

Lentiviral

Promoter

UbC

Available Since

June 30, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
Lenti-FAB(ER-LD)

Plasmid

#214420

Purpose

Detection of ER-lipid droplet contact site

Depositor

Chi-Lun Chang

Article

Li et al J Cell Biol. 2024 Sep 2;223(9):e20231112

Insert

RspAN-FRB-V5-ER_IRES_CFAST-20nm-Halo-6xHp

Use

Lentiviral

Tags

CFAST-Halo and RspAN-FRB-V5

Promoter

CMV

Available Since

March 20, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pCas9-HE-Geminin

Plasmid

#109402

Purpose

Expresses Cas9 fusion to HE (HDR-enhancer) domain of human CtIP protein and Geminin degron

Depositor

Jean-Paul Concordet

Article

Charpentier et al Nat Commun. 2018 Mar 19;9(1):1133. doi:

Insert

HE-Geminin

Available Since

June 15, 2018

Availability

Academic Institutions and Nonprofits only

Enlarge
pEVOL-tac-EcTyrRS-pAAFRS-9-lpp-tRNA_CUA^EcTyr

Plasmid

#218767

Purpose

encodes EcTyrRS-pAAFRS-9 and EcTyr-tRNA-TAG

Depositor

Abhishek Chatterjee

Article

Ficaretta et al ACS Chem Biol. 2024 Jun 24. doi: 10.1021

Insert

EcTyrRS-pAAFRS-9

Expression

Bacterial

Mutation

Y37G, L71V, D182C, F183Y, L186C, D265R

Promoter

tacI

Available Since

June 28, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
pET21-pelB-LaMIgG-14

Plasmid

#228994

Purpose

For bacterial expression of anti-mouse IgG nanobody LaMIgG-14, with pelB leader for periplasmic secretion, and C-term free cysteine and C-term 6xHIS tag.

Depositor

Michael Rout

Article

Fridy et al J Biol Chem. 2024 Aug 2;300(9):107623. d

Insert

LaMIgG-14

Tags

6xHIS

Expression

Bacterial

Promoter

T7

Available Since

Jan. 13, 2025

Availability

Academic Institutions and Nonprofits only

Enlarge
NAX_CAGGS_CAS9_m4_KRAB-MeCP2_KanR neo

Plasmid

#167901

Purpose

PiggyBac compatible plasmid expressing dCas9-KRAB-MeCP2 expression plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Insert

dCas9-KRAB-MeCP2

Use

CRISPR

Mutation

D10A, D839A, H840A, N863A

Available Since

Sept. 12, 2022

Availability

Academic Institutions and Nonprofits only

Enlarge
pEC3104 (pSpy1C-PgyrA(Sag)_ermBL-ermB'~ffluc)

Plasmid

#218513

Purpose

replicative E. coli-S. pyogenes shuttle plasmid for erythromycin-inducible expression of the Firefly luciferase

Depositor

Emmanuelle Charpentier

Article

Lautenschlager et al Front Bioeng Biotechnol. 2024 Jun 7;12:1

Insert

ffluc

Use

Luciferase

Expression

Bacterial

Mutation

deletion of the Plac_mrfp cassette

Promoter

PgyrA_ermBL-ermB'

Available Since

Sept. 3, 2024

Availability

Academic Institutions and Nonprofits only

Enlarge
Mfn2(K109A)-16xmyc

Plasmid

#26051

Depositor

David Chan

Article

Chen et al J Cell Biol. 2003 Jan 20. 160(2):189-200

Insert

Mfn2 (Mfn2 Mouse)

Tags

16xMyc

Expression

Mammalian

Mutation

K109A GTPase mutant

Available Since

Aug. 20, 2010

Availability

Academic Institutions and Nonprofits only
pExodus CMV.Trex2

Plasmid

#40210

Purpose

Mammalian expression of codon-optimized Trex2.

Depositor

Andrew Scharenberg

Article

Certo et al Nat Methods. 2012 Oct;9(10):973-5. doi:

Insert

Three prime repair exonuclease 2 (Trex2 Mouse)

Expression

Bacterial and Mammalian

Promoter

CMV and T7

Available Since

Sept. 27, 2012

Availability

Academic Institutions and Nonprofits only

Enlarge