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pcDNA-GreenPy1Lowest

Plasmid

#239062

Purpose

Mammalian expression of lowest affinity green fluorescent pyruvate biosensor GreenPy1Lowest

Depositor

Robert Campbell

Article

Imai et al bioRxiv 2025.04.17.649293

Insert

GreenPy1Lowest

Expression

Mammalian

Available Since

June 18, 2025

Availability

Academic Institutions and Nonprofits only

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pMAH2-intein/mCherry

Plasmid

#71405

Purpose

Expresses suppression tRNA and mCherry inserted with a Npu DnaE intein

Depositor

Huiwang Ai

Article

Ren et al J Am Chem Soc. 2015 Feb 18;137(6):2155-8

Insert

Npu DnaE Intein insertion in mCherry

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 16, 2016

Availability

Academic Institutions and Nonprofits only
pBFv-U6.2B

Plasmid

#138401

Purpose

gRNA expression vector; Can be combined with pBFv-U6.2 to generate double-gRNA expression vector

Depositor

Shu Kondo

Article

Kondo et al Genetics. 2013 Nov;195(3):715-21. doi: 1

Insert

U6-2

Expression

Insect

Promoter

U6-2

Available Since

March 19, 2020

Availability

Academic Institutions and Nonprofits only

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pET-Gate2 mazEF

Plasmid

#117117

Purpose

Destination vector with mazEF toxin antitoxin system

Depositor

Stephan Gruber

Article

Diebold-Durand et al Methods Mol Biol. 2019;2004:49-61. doi:

Type

Empty backbone

Use

Backbone for golden gate assembly for gene target…

Available Since

Nov. 14, 2018

Availability

Academic Institutions and Nonprofits only

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OCRL1-pmCherryC1

Plasmid

#27675

Depositor

Christien Merrifield

Article

Taylor et al PLoS Biol. 2011 Mar . 9(3):e1000604.

Insert

OCRL1 (Ocrl Mouse)

Tags

mCherry

Expression

Mammalian

Available Since

May 4, 2011

Availability

Academic Institutions and Nonprofits only
p15A-OptoCre-knt-P*-R

Plasmid

#189850

Purpose

Cre-inducible activation of knt for kanamycin resistance.

Depositor

Mary Dunlop

Article

Sheets et al Nat Commun. 2023 Feb 23;14(1):1034. doi:

Insert

P*-loxP-TT-loxP-R-knt

Use

Cre/Lox and Synthetic Biology

Expression

Bacterial

Promoter

P* (modified T7 phage gene 10)

Available Since

Feb. 21, 2023

Availability

Academic Institutions and Nonprofits only

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Ai3

Plasmid

#22797

Depositor

Hongkui Zeng

Article

Madisen et al Nat Neurosci. 2010 Jan . 13(1):133-40.

Insert

CAG-Floxed EYFP

Use

Mouse Targeting

Available Since

Dec. 17, 2009

Availability

Academic Institutions and Nonprofits only

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pAd5-B3

Plasmid

#122553

Purpose

Block 3 for AdenoBuilder genome assembly

Depositor

Fred Bunz

Article

Miciak et al PLoS One. 2018 Jun 27;13(6):e0199563. do

Insert

Adenovirus 5 genomic region 10862-14499

Use

Synthetic Biology

Available Since

April 23, 2021

Availability

Academic Institutions and Nonprofits only

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pGFAP::MyrGFP_v2

Plasmid

#160277

Purpose

Expresses GFP from the Gfa2 promoter. This plasmid is a new version of Plasmid 22672 and includes a stop codon in the myrEGFP insert.

Depositor

Robert Benezra

Article

Nam et al Cell Stem Cell. 2009 Nov 6. 5(5):515-26.

Insert

myrGFP

Expression

Mammalian

Promoter

Gfa2

Available Since

Nov. 10, 2020

Availability

Academic Institutions and Nonprofits only

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PA-GFPmt

Plasmid

#19989

Depositor

Orian Shirihai

Article

Twig et al Am J Physiol Cell Physiol. 2006 Jul . 29

Insert

GFP

Use

Lentiviral

Available Since

Sept. 17, 2009

Availability

Academic Institutions and Nonprofits only