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pKLV-EF1a-BFP-W

Plasmid

#208757

Purpose

Lentiviral vector expressing BFP

Depositor

Kosuke Yusa

Article

Aoki et al Blood. 2023 Nov 3:blood.2023020857. doi:

Type

Empty backbone

Use

Lentiviral

Available Since

Jan. 17, 2024

Availability

Academic Institutions and Nonprofits only

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Ac5-STABLE2-puro

Plasmid

#208032

Purpose

Enables expression of 2 ORFs (with or without tags) and selection with puromycin in insect cells. Includes Flag-mCherry-T2A-GFP-T2A-puro with Actin5C promoter.

Depositor

Rosa Barrio, James Sutherland

Article

Gonzalez et al Sci Rep. 2011;1:75. doi: 10.1038/srep000

Type

Empty backbone

Expression

Insect

Available Since

Oct. 11, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.4_mCherry-MMLV_Psi

Plasmid

#205522

Purpose

Express mCherry-Psi (export tag consisting of MMLV Psi)

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

mCherry

Expression

Mammalian

Mutation

WT

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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GPI-mNeonGreen

Plasmid

#197912

Purpose

Monomer expression vector with plasma membrane localization signal

Depositor

Salvatore Chiantia

Article

Petrich et al PLoS One. 2023 Aug 3;18(8):e0285486. doi

Insert

glycosylphosphatidylinositol-mNeonGreen

Expression

Mammalian

Mutation

none

Available Since

April 19, 2023

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA mcherry

Plasmid

#167910

Purpose

Lentiviral vector for expressing U6 sgRNA cloning plasmid

Depositor

Alejandro Chavez, Nan Cher Yeo

Article

Nghi Dang et al Synthetic Biology, Volume 6, Issue 1, 20

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

Sept. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pHR_TRE3GS-->sGFP-P2A-mCherry

Plasmid

#162232

Purpose

Lentiviral vector for the expression of TRE3GS-->sGFP-P2A-mCherry in mammalian cells

Depositor

Wendell Lim

Article

Toda et al Science. 2020 Oct 16;370(6514):327-331.

Insert

EGFP and mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

June 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMT mCherry-CaaX

Plasmid

#126669

Purpose

Inducible expression of mCherry-CaaX in S2 cells

Depositor

Ron Vale

Article

Williamson et al J Cell Biol. 2018 Nov 5;217(11):3977-399

Insert

mCherry-CaaX

Expression

Insect

Available Since

Jan. 18, 2022

Availability

Academic Institutions and Nonprofits only

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pCS4092

Plasmid

#131741

Purpose

Theophylline switch Theo-CAUAA

Depositor

Christina Smolke

Article

Xiang et al Nat Commun. 2019 Sep 23;10(1):4327. doi:

Insert

Theophylline switch Theo-CAUAA

Expression

Mammalian

Available Since

June 9, 2021

Availability

Academic Institutions and Nonprofits only

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pCPP5371

Plasmid

#128732

Purpose

Gateway destination low-copy number plasmid for expression of any effector from avrPto promoter with C-terminal Cya tag

Depositor

Alan Collmer

Article

Oh et al J Bacteriol. 2007 Nov;189(22):8277-89. E

Type

Empty backbone

Expression

Bacterial

Available Since

Jan. 21, 2021

Availability

Academic Institutions and Nonprofits only

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pLITMUS*-PrpoN-Cro-PBBa_J23106-geneIII

Plasmid

#134353

Purpose

Phagemid

Depositor

Mark Isalan

Article

Brodel et al Sci Adv. 2020 Jun 10;6(24):eaba2728. doi

Insert

Cro

Expression

Bacterial

Available Since

June 11, 2020

Availability

Academic Institutions and Nonprofits only

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RV-mCD4-HH5-1

Plasmid

#124961

Purpose

Express the Helicobacter hepaticus specific TCRa/b HH5-1 in T cell hybridoma cells

Depositor

Dan Littman

Article

Xu et al Nature. 2018 Feb 15;554(7692):373-377. d

Insert

HH5-1

Use

retroviral

Expression

Mammalian

Available Since

June 6, 2019

Availability

Academic Institutions and Nonprofits only

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pWT037a

Plasmid

#107896

Purpose

W2.1 plasmid. Contains PTetO-SD8-BE2, PLacO-sgRNA1 and is part of CAMERA 2.1

Depositor

David Liu

Article

Tang et al Science. 2018 Feb 15. pii: science.aap89

Insert

PTetO-SD8-BE2, PLacO-sgRNA1

Expression

Bacterial

Available Since

April 17, 2018

Availability

Academic Institutions and Nonprofits only

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L40C-CRISPR.EFS.PAC

Plasmid

#89393

Purpose

Lentiviral CRISPR-Cas9 delivery for SpCas9 and sgRNA (hU6), PAC (Puromycin resistance) coexpression, EFS Promoter driven

Depositor

Dirk Heckl

Article

Reimer et al Haematologica. 2017 Jun 1. pii: haematol

Type

Empty backbone

Use

CRISPR and Lentiviral

Available Since

June 5, 2017

Availability

Academic Institutions and Nonprofits only

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pGAPTrap-GFP

Plasmid

#82506

Purpose

Vector targeting the eGFP gene to the GAPDH locus of human cells. eGFP is expressed from a 2A sequence at the C terminus of GAPDH.

Depositor

Ed Stanley

Article

Kao et al Stem Cell Reports. 2016 Sep 1. pii: S221

Insert

eGFP

Use

Synthetic Biology

Expression

Mammalian

Available Since

Sept. 30, 2016

Availability

Academic Institutions and Nonprofits only

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pKF297

Plasmid

#74774

Purpose

template for C-terminal TEV-site + 2x V5-tag (short version), Puromycin-Resistance

Depositor

Klaus Foerstemann

Article

Kunzelmann et al G3 (Bethesda). 2016 Jun 1;6(6):1777-85.

Insert

TEV- 2xV5 tag (short version)

Expression

Insect

Available Since

April 28, 2016

Availability

Academic Institutions and Nonprofits only

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mVenus-tdTomato-FRET-10

Plasmid

#58156

Purpose

Localization: Cytosolic FRET, Excitation: / 554, Emission: / 581

Depositor

Michael Davidson

Type

Empty backbone

Tags

mVenus and tdTomato

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
mPAGFP-mCherry-FRET-10

Plasmid

#58166

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

mCherry and mPAGFP

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
mTagBFP2-mEGFP-FRET

Plasmid

#58173

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

mEGFP and mTagBFP2

Expression

Mammalian

Available Since

April 28, 2015

Availability

Industry, Academic Institutions, and Nonprofits
Cerulean-Venus-FRET-10

Plasmid

#58177

Purpose

Localization: Cytosolic FRET, Excitation: , Emission:

Depositor

Michael Davidson

Type

Empty backbone

Tags

Cerulean and Venus

Expression

Mammalian

Available Since

April 28, 2015

Availability

Academic Institutions and Nonprofits only
pMTB-Multibow-mfR

Plasmid

#60991

Purpose

Part of Multibow set of constructs--each FP gene is initially not expressed and then adopts a permanent ON or OFF status upon Cre-mediated recombination. Encodes membrane bound mKate2.

Depositor

Sean Megason

Article

Xiong et al PLoS One. 2015 May 26;10(5):e0127822. do

Insert

mKate2

Use

Zebrafish

Tags

membrane tag

Promoter

Bactin2

Available Since

Jan. 22, 2015

Availability

Academic Institutions and Nonprofits only