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pCEP4-hERG1a

Plasmid

#53052

Purpose

Expresses alpha subunit of hERG1a K channel in mammalian cells

Depositor

Michael Sanguinetti

Article

Mitcheson et al Proc Natl Acad Sci U S A. 2000 Oct 24;97

Insert

Potassium voltage-gated channel subfamily H member 2 (KCNH2 Human)

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 2, 2014

Availability

Academic Institutions and Nonprofits only

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LentiV_Cas9_puro

Plasmid

#108100

Purpose

Lentiviral expression plasmid of spCas9 with puromycin resistance gene

Depositor

Christopher Vakoc

Article

Tarumoto et al Mol Cell. 2018 Feb 28. pii: S1097-2765(1

Insert

Cas9

Use

CRISPR and Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

EFS promoter

Available Since

July 5, 2018

Availability

Academic Institutions and Nonprofits only

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TFORF2693

Plasmid

#142359

Purpose

Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

Depositor

Feng Zhang

Article

Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

Insert

TEAD4 (TEAD4 Human)

Use

Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

June 20, 2023

Availability

Academic Institutions and Nonprofits only

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pCEP4-HA-BAX

Plasmid

#16587

Depositor

Bert Vogelstein

Article

Yu et al Mol Cell. 2001 Mar . 7(3):673-82.

Insert

BAX alpha (BAX Human)

Tags

2x HA

Expression

Mammalian

Available Since

March 10, 2008

Availability

Academic Institutions and Nonprofits only

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pGEM4Z-T7-5'UTR-mGreenLantern-3'UTR-A64

Plasmid

#203349

Purpose

Template for T7 in vitro transcription of mGreenLantern mRNA flanked by Xenopus beta globin UTRs and 64nt polyA tail

Depositor

Christopher Grigsby, Molly Stevens

Insert

mGreenLantern

Use

Template for t7-mediated in vitro transcription o…

Tags

Flanked by 5' and 3' UTRs from Xenopus …

Promoter

T7

Available Since

June 29, 2023

Availability

Academic Institutions and Nonprofits only

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pAAV/mIba1.GFP.WPRE.miR-9.T.miR-129-2-3p.T.SV40pA

Plasmid

#190163

Purpose

Microglia-targeted transgene expression.

Depositor

Hirokazu Hirai

Article

Okada et al Commun Biol. 2022 Nov 11;5(1):1224. doi:

Inserts

mIba1 promoter, 1.7-kb, microglia-specific promoter
miR-9-Tx4, 4 repeated miRNA target sites for miR-9
miR-129-2-3p-Tx4, 4 repeated miRNA target sites for miR-129-2-3p

Use

AAV

Available Since

Nov. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pCF160_VSV-G

Plasmid

#225963

Purpose

CMV-Intron-VSVG (env protein). Expresses VSV-G env for VLP production.

Depositor

Christof Fellmann

Article

Ramadoss et al Nat Commun. 2025 Nov 17;16(1):9883. doi:

Insert

CMV-Intron-VSVG (env protein)

Expression

Mammalian

Available Since

Oct. 30, 2024

Availability

Academic Institutions and Nonprofits only

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Puro-Cas9 donor

Plasmid

#58409

Purpose

Donor vector for genomic targeting of a Tetracycline-inducible Cas9 cassette to the human AAVS1/PPP1R12C locus

Depositor

Danwei Huangfu

Article

Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

Insert

hSpCas9

Use

CRISPR

Tags

3xFlag, Nucleoplasmin NLS, and SV40 NLS

Expression

Mammalian

Available Since

Sept. 17, 2014

Availability

Academic Institutions and Nonprofits only

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EF1a-L274GMmMetRS-T2A-mCherry

Plasmid

#220800

Purpose

Lentivirus expressing mutant tRNA synthetase for incorporation of noncanonical amino acids in nascent proteins plus mCherry reporter and puro resistance

Depositor

Elizabeth Kirby

Article

Sridharan et al Stem Cell Res Ther. 2024 Jun 26;15(1):18

Insert

Mars1 (Mars1 Mouse)

Use

Lentiviral

Tags

2xFLAG and T2A-mCherry

Mutation

mutation in MetRS to change aa 274 from L to G

Promoter

EF1a

Available Since

June 27, 2024

Availability

Academic Institutions and Nonprofits only

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pAAV-Ef1a-DO_DIO-TdTomato_EGFP-WPRE-pA

Plasmid

#37120

Purpose

DO/DIO Cre-Switch. Expresses tdTomato in Cre negative cells, expresses EGFP in Cre positive cells

Depositor

Bernardo Sabatini

Article

Saunders et al Front Neural Circuits. 2012 Jul 27;6:47.

Insert

TdTomato-EGFP

Use

AAV and Cre/Lox; Cre-switch

Promoter

EF1a

Available Since

July 27, 2012

Availability

Academic Institutions and Nonprofits only

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