We narrowed to 12,121 results for: VARS

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MYMH 327

Plasmid

#138054

Purpose

Encodes Ribosome Binding Site (RBS) variant using GFP as a reporter gene

Depositor

Meng How Tan

Article

Teh et al ACS Synth Biol. 2019 Apr 19;8(4):708-723

Insert

pSEVA-331Bb-1AG 2AC-GFP

Expression

Bacterial

Mutation

GCA GAG GAG AAA

Available Since

April 10, 2020

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbu

Plasmid

#129637

Purpose

Expression plasmid of Anbu from Y. bercovieri (UNIPROT A0A0T9RXH3 variant with V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbu

Tags

His6-tag

Expression

Bacterial

Mutation

V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET20b-YbAnbuT1A

Plasmid

#129638

Purpose

Expression plasmid of Anbu from Y. bercovieri (inactive mutant; (UNIPROT A0A0T9RXH3 variant with T1A, V14A and G107H mutations)

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbAnbuT1A

Tags

His6-tag

Expression

Bacterial

Mutation

T1A, V14A and G107H

Promoter

T7

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pET41a iGluSnFR E25R

Plasmid

#119831

Purpose

Production of recombinant iGluSnFR E25R variant, a single-wavelength glutamate indicator, in E. coli

Depositor

Katalin Torok

Article

Helassa et al Proc Natl Acad Sci U S A. 2018 May 7. pi

Insert

iGluSnFR E25R

Tags

GST

Expression

Bacterial

Mutation

E25R

Promoter

T7

Available Since

March 12, 2019

Availability

Academic Institutions and Nonprofits only

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F. prausnitzii 16S toehold switch sensor

Plasmid

#110705

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

F. prausnitzii 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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R. hominis 16S toehold switch sensor

Plasmid

#110703

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

R. hominis 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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E. rectale 16S toehold switch sensor

Plasmid

#110702

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

E. rectale 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B. breve 16S toehold switch sensor

Plasmid

#110701

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. breve 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B. adolescentis 16S toehold switch sensor

Plasmid

#110700

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. adolescentis 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B. longum 16S toehold switch sensor

Plasmid

#110699

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. longum 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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B. thetaiotaomicron 16S toehold switch sensor

Plasmid

#110697

Purpose

Toehold switch sensor to detect the V3 hypervariable region of the 16S rRNA with GFP output

Depositor

James Collins

Article

Takahashi et al Nat Commun. 2018 Aug 21;9(1):3347. doi:

Insert

B. thetaiotaomicron 16S toehold switch sensor

Use

Synthetic Biology

Promoter

T7

Available Since

Sept. 4, 2018

Availability

Academic Institutions and Nonprofits only

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SCRIB_PDZ_2

Plasmid

#103884

Purpose

Protein expression and purification of Scribble PDZ2 domain

Depositor

Sachdev Sidhu

Article

Tonikian et al PLoS Biol. 2008 Sep 30;6(9):e239. doi: 1

Insert

SCRIB (SCRIB Human)

Tags

GST

Expression

Bacterial

Promoter

tac

Available Since

Aug. 29, 2018

Availability

Academic Institutions and Nonprofits only

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HA-mus-Bnip3_deltaExon3

Plasmid

#100793

Purpose

Mammalian expression of Bnip3_deltaExon3 splice variant

Depositor

Joseph Gordon

Article

Diehl-Jones et al J Pediatr Gastroenterol Nutr. 2015 Nov;6

Insert

mouse-Bnip3_deltaExon3 (Bnip3 Mouse)

Tags

HA

Expression

Mammalian

Mutation

Exon3 deletion

Promoter

CMV

Available Since

Sept. 11, 2017

Availability

Academic Institutions and Nonprofits only

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pGS-3R112C/T180C

Plasmid

#85095

Purpose

Host vector to fuse a gene at the N-terminus of PCNA3 variant (R112C/T180C)

Depositor

Teruyuki Nagamune

Article

Tan et al Angew Chem Int Ed Engl. 2016 Oct 26. doi

Insert

DNA polymerase sliding clamp 3

Tags

His6 tag

Expression

Bacterial

Mutation

changed Arginine 112 to Cysteine and Threonine 18…

Promoter

T7 promoter

Available Since

Nov. 29, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode81

Plasmid

#83758

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode81

Use

Retroviral

Mutation

deletion of T in starting ATG of GFP (please see …

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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MSCV-6NBC-GFP-PGK-Puro-Barcode71

Plasmid

#83748

Purpose

individual 6N barcode to make barcoded cell line variants

Depositor

Monte Winslow

Article

Gruner et al Nat Methods. 2016 Sep 12. doi: 10.1038/n

Insert

Barcode71

Use

Retroviral

Mutation

deletion of AA5-233 in GFP (please see depositor …

Available Since

Nov. 10, 2016

Availability

Academic Institutions and Nonprofits only

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pSB700 lenti gRNA Cerulean-zhang2.0-Weiss-chimera2

Plasmid

#79380

Purpose

Cas9 gRNA variant to recruit ms2 proteins

Depositor

George Church

Article

Chavez et al Nat Methods. 2016 May 23. doi: 10.1038/n

Insert

Chimera 2

Use

CRISPR and Lentiviral

Expression

Mammalian

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5L18-NtermN3

Plasmid

#51448

Purpose

Expresses TALEN targeting left CCR5A site (L18) with N3 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Left (L18) N3 N-term

Use

TALEN

Tags

3xFLAG and Fok1 EL variant

Expression

Mammalian

Mutation

K150Q, K153Q, and R154Q

Promoter

CMV

Available Since

May 21, 2014

Availability

Academic Institutions and Nonprofits only

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TALEN-CCR5R18-NtermN3

Plasmid

#51449

Purpose

Expresses TALEN targeting right CCR5A site (R18) with N3 N-terminal domain

Depositor

David Liu

Article

Guilinger et al Nat Methods. 2014 Apr;11(4):429-35. doi:

Insert

TALEN CCR5 Right (R18) N3 N-term

Use

TALEN

Tags

3xFLAG and Fok1 KK variant

Expression

Mammalian

Mutation

K150Q, K153Q, and R154Q

Promoter

CMV

Available Since

April 1, 2014

Availability

Academic Institutions and Nonprofits only

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refGFP1_pQE30

Plasmid

#48644

Purpose

Bact exp vector encoding for the 6XHis tagged insensitive variant of the fluorescent redox probe roGFP with mut Cys147 to Serine, del insertion147b and mutated Cys204 to Glut.Referred to as refGFP

Depositor

David Ron

Article

Avezov et al J Cell Biol. 2013 Apr 15;201(2):337-49.

Insert

refGFP1

Tags

6xHis

Expression

Bacterial

Mutation

refGFP C147S, C204Q

Available Since

Oct. 23, 2013

Availability

Academic Institutions and Nonprofits only

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