We narrowed to 12,201 results for: pcDNA

Showing: 10541 - 10560 of 12201 results

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Cas9-FCPF

Plasmid

#220141

Purpose

Expresses Cas9-FCPF in mammalian cells

Depositor

Xinlai Cheng

Article

Gama-Brambila et al JACS Au. 2021 May 12;1(6):777-785. doi:

Insert

Cas9-FCPF (cas9 Synthetic, S. Pyogenes)

Tags

Flag

Expression

Mammalian

Available Since

July 17, 2024

Availability

Academic Institutions and Nonprofits only

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pPL6873_2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Plasmid

#217970

Purpose

Provides expression from a CMV promoter surrounded by insulators. Integrates at FRT site. Confers puromycin resistance. Expresses nuclear localized BFP.

Depositor

Robert Piper

Article

Minard et al Biol Open. 2025 Oct 15;14(10):bio061793.

Insert

2xcHS4::mCherry::2xcHS4_FRT-PuroR-P2A-mTagBFP2-NLS

Expression

Mammalian

Available Since

June 13, 2024

Availability

Academic Institutions and Nonprofits only

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2x3APWWP-IFP2.0C

Plasmid

#215752

Purpose

Expresses double domain of DNMT3A PWWP domain fused to C-terminal part of IFP2.0 for H3K36me2/3 detection in BiAD sensors

Depositor

Albert Jeltsch

Article

Kohler et al Cell Rep Methods. 2024 Mar 26:100739. do

Insert

2x3APWWP-IFP2.0C

Expression

Mammalian

Promoter

CMV

Available Since

April 16, 2024

Availability

Academic Institutions and Nonprofits only

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NSP3C-FR-2Strep

Plasmid

#210312

Purpose

Expresses the C terminal of SARS-CoV-2 NSP3 in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

C terminal of SARS-CoV-2 NSP3 (ORF1ab )

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 3, 2024

Availability

Academic Institutions and Nonprofits only

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SARS-CoV-2 N-2Strep

Plasmid

#210376

Purpose

Expresses SARS-CoV-2 N protein fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

SARS-COV-2 N (N SARS-CoV-2)

Tags

2Strep

Expression

Mammalian

Available Since

Jan. 2, 2024

Availability

Academic Institutions and Nonprofits only

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TLNRD1_2T_eGFP

Plasmid

#208883

Purpose

Express eGFP-TLNRD1 with the L191T and A225T mutations in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutations L191T and A225T

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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TLNRD1_2E_eGFP

Plasmid

#208884

Purpose

Express eGFP-TLNRD1 with the K192E and R233E mutations in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Ball et al J Cell Biol. 2024 Sep 2;223(9):e20231003

Insert

TLNRD1 (TLNRD1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

mutations K192E and R233E

Available Since

Dec. 22, 2023

Availability

Academic Institutions and Nonprofits only

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REEP6-2Strep

Plasmid

#210351

Purpose

Expresses REEP6 fused with 2Strep in mammalian cells

Depositor

Michele Pagano

Article

Li et al J Virol. 2023 Sep 28:e0050723. doi: 10.1

Insert

REEP6 (REEP6 Human)

Tags

2Strep

Expression

Mammalian

Available Since

Dec. 19, 2023

Availability

Academic Institutions and Nonprofits only

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pHDR-PMG (pXK-1650)

Plasmid

#208858

Purpose

HDR-base selectable traffic light reporter (TLR) for helping to screen HDR-editing positive cells. Key Construct: iPuroR*-CMV-PuroR.L-mCherry-PuroR.R-GFP.

Depositor

Kun Xu

Article

Lyu et al Int J Biol Macromol. 2023 Jul 15;243:124

Type

Empty backbone

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 14, 2023

Availability

Academic Institutions and Nonprofits only

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MCP-mCherry-TIS11B

Plasmid

#207670

Purpose

To express fluorescently labeled MS2 coat binding protein (MCP) fused with RNA binding protein TIS11B

Depositor

Christine Mayr

Article

Horste et al Mol Cell. 2023 Dec 21;83(24):4509-4523.e

Insert

TIS11B (ZFP36L1 Human)

Tags

MCP-mCherry

Expression

Mammalian

Available Since

Oct. 4, 2023

Availability

Academic Institutions and Nonprofits only

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pFH2.17_GagZip-MCP-dpol-P2A-eGFP

Plasmid

#205532

Purpose

Express HIV GagZip-MCP-dpol-P2A-eGFP

Depositor

Michael Elowitz

Article

Horns et al Cell. 2023 Jul 10:S0092-8674(23)00689-X.

Insert

Gag-MCP-dpol

Expression

Mammalian

Mutation

Nucleocapsid replaced with leucine zipper, pol fr…

Promoter

CMV

Available Since

Sept. 26, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F4

Plasmid

#194863

Purpose

Express the RAPH1 fragment (1062-1250) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 1062-1250

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F3

Plasmid

#194862

Purpose

Express the RAPH1 fragment (868-1062) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 868-1062

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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eGFP-RAPH1-F2

Plasmid

#194861

Purpose

Express the RAPH1 fragment (535-868) tagged to eGFP in mammalian cells.

Depositor

Guillaume Jacquemet

Article

Popovic et al J Cell Sci. 2023 Mar 1;136(5):jcs260574.

Insert

RAPH1 (RAPH1 Human)

Tags

eGFP

Expression

Mammalian

Mutation

amino acids 535-868

Promoter

CMV

Available Since

March 28, 2023

Availability

Academic Institutions and Nonprofits only

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pClathrin-miRFP718nano

Plasmid

#195747

Purpose

Clathrin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-clathrin light chain

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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pTubulin-miRFP718nano

Plasmid

#195746

Purpose

Human alpha-tubulin labelled with near-infrared fluorescent protein miRFP718nano

Depositor

Vladislav Verkhusha

Article

Oliinyk et al Nat Methods. 2022 Dec 1. doi: 10.1038/s4

Insert

miRFP718nano-human alpha-tubulin

Tags

miRFP718nano

Expression

Mammalian

Promoter

CMV

Available Since

Feb. 17, 2023

Availability

Academic Institutions and Nonprofits only

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CAGGS-AsCpf1-2A-GFP-U6-AAVS1-MAFB-sgRNA

Plasmid

#194725

Purpose

based on (CAGGS-AsCpf1-2A-GFP-U6-sgRNA-cloning vector, Addgene 159281), with guide RNA array targeting both AAVS1 and MAFB

Depositor

Rudolf Jaenisch

Article

Haiting et al bioRxiv

Insert

AsCpf1 (MAFB, AAVS1 Acidaminococcus sp.)

Use

CRISPR

Expression

Mammalian

Mutation

D908A

Available Since

Jan. 5, 2023

Availability

Academic Institutions and Nonprofits only

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GFP-CBS (H65R)

Plasmid

#182924

Purpose

Mammalian expression plasmid for human CBS mutant H65R

Depositor

Philip Eaton

Article

Switzer et al Proc Natl Acad Sci U S A. 2023 Jan 17;12

Insert

Cystathionine Beta-Synthase (CBS Human)

Tags

eGFP

Expression

Mammalian

Mutation

changed histidine 65 to arginine

Promoter

CMV

Available Since

Jan. 3, 2023

Availability

Academic Institutions and Nonprofits only

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FLAG-mVenusC-14-HP1b Chromo W42A

Plasmid

#191399

Purpose

W42A binding pocket mutant of HP1b chromo domain fused to C-terminal part of split mVenus

Depositor

Albert Jeltsch

Article

Lungu et al Nat Commun. 2017 Sep 21;8(1):649. doi: 1

Insert

FLAG-mVenusC-14-HP1b Chromo W42A

Tags

3xFLAG

Expression

Mammalian

Mutation

W42A

Promoter

CMV

Available Since

Dec. 19, 2022

Availability

Academic Institutions and Nonprofits only

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nPPARg429-gs10-NLS-VPR

Plasmid

#188266

Purpose

Plasmid ensures constitutive expression of the N-terminal fragment of split PPARgamma (aa 200-429), fused to the VPR activation domain, a flexible GS linker and the SV40 NLS signal at the C-terminus

Depositor

Roman Jerala

Article

Rihtar et al Nat Chem Biol. 2022 Sep 26. doi: 10.1038

Insert

nPPARg429 (PPARG Human)

Expression

Mammalian

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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