We narrowed to 4,241 results for: Pet2;

1. pET28a-mH6-Cas12i1

   Plasmid

   #120882

   Purpose

   Expresses E. coli codon optimized Cas12i1 effector in the pET28a backbone.

   Depositor

   Arbor Biotechnologies

   Article

   Yan et al Science. 2019 Jan 4;363(6422):88-91. doi

   Insert

   Cas12i1
   

   Use

   CRISPR

   Tags

   mH6

   Expression

   Bacterial

   Mutation

   WT

   Promoter

   lac

   Available since

   Feb. 15, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET21-pelB-LaG-30

   Plasmid

   #172754

   Purpose

   Bacterial expression of HIS-tagged anti-GFP nanobody LaG-30; HIS tag preceded by HRV 3C/PreScission cleavage site.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaG-30
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET28-SpyCatcher002-MBP

   Plasmid

   #216295

   Purpose

   Expresses SpyCatcher002 linked to maltose-binding protein in bacterial cells

   Depositor

   Mark Howarth

   Article

   Driscoll et al Nat Commun. 2024 Mar 16;15(1):2403. doi:

   Insert

   SpyCatcher002-MBP
   

   Use

   Tags

   His6 tag and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   June 26, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pET21a-hAIM2-His6

   Plasmid

   #51532

   Purpose

   Express His6-tagged hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Use

   Tags

   His6 and T7

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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5. pET28a-SpyCatcher-mi3

   Plasmid

   #112255

   Purpose

   Expresses SpyCatcher-mi3 in bacterial cells as platform for plug-and-display vaccination

   Depositor

   Mark Howarth

   Article

   Bruun et al ACS Nano. 2018 Jul 20. doi: 10.1021/acsn

   Insert

   SpyCatcher-mi3
   

   Use

   Tags

   C-tag

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Sept. 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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6. pET28b-6His-AcpS

   Plasmid

   #145376

   Purpose

   Expression of E. coli AcpS

   Depositor

   Robert Tomko

   Article

   Nemec et al Yeast. 2020 May 13. doi: 10.1002/yea.347

   Insert

   AcpS (acpP E. coli)
   

   Use

   Tags

   6-His

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   May 14, 2020

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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7. pET28a T7pCONS sfGFP

   Plasmid

   #154462

   Purpose

   pET28a with a design flaw in the T7 promoter fixed. Contains the coding sequence for sfGFP

   Depositor

   Daniel Daley

   Article

   Shilling et al Commun Biol. 2020 May 7;3(1):214. doi: 1

   Insert

   super folder GFP
   

   Use

   Tags

   His-thrombin

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET21a-hAIM2-GST

   Plasmid

   #51528

   Purpose

   Express GST-tagged hAIM2 in bacteria

   Depositor

   Emad Alnemri

   Article

   Fernandes-Alnemri et al Nature. 2009 Mar 26;458(7237):509-13. do

   Insert

   AIM2 (AIM2 Human)
   

   Use

   Tags

   GST

   Expression

   Bacterial

   Mutation

   Promoter

   lac operator

   Available since

   March 11, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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9. pET21-pelB-LaM-8

   Plasmid

   #172772

   Purpose

   Bacterial expression of anti-mCherry nanobody LaM-8, with pelB leader and C-term free cysteine and 6xHIS tag.

   Depositor

   Michael Rout

   Article

   Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

   Insert

   LaM-8
   

   Use

   Tags

   6xHIS and pelB leader for periplasmic secretion

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   Aug. 18, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pET28-Dnmt3a3L-sc27

    Plasmid

    #71827

    Purpose

    For bacterial expression of Dnmt3a-Dnmt3L single-chain fusion protein protein. Contains the catalytic domain of mouse Dnmt3a and the C-terminal part of mouse Dnmt3L connected by 27 aa linker.

    Depositor

    Albert Jeltsch

    Article

    Siddique et al J Mol Biol. 2013 Feb 8;425(3):479-91. do

    Insert

    Dnmt3a catalytic domain (M. musculus) Dnmt3L (M. musculus) C-terminal domain gene fusion (Dnmt3a Mouse)
    

    Use

    Tags

    6xHis tag

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 15, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. pET28C-mCherry-YTHDF2

    Plasmid

    #204406

    Purpose

    E. coli expression of full-length YTHDF2 with an N-terminal mCherry tag

    Depositor

    Samie Jaffrey

    Article

    Dumelie et al Nat Chem Biol. 2023 Nov 16. doi: 10.1038

    Insert

    YTHDF2 (YTHDF2 Human)
    

    Use

    Tags

    6x-His; mCherry

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Sept. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a-BCA-Q11

    Plasmid

    #185781

    Purpose

    Encodes enzyme bovine carbonic anhydrase linked to Q11 self-assembling peptide via a GS-linker sequence

    Depositor

    Lizhong He

    Article

    Shanbhag et al Adv. Funct. Mater. 2022, 32, 2108039

    Insert

    BCA-Q11
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    July 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pet26-MBP-ELIC

    Plasmid

    #39239

    Depositor

    Raimund Dutzler

    Article

    Hilf et al Nature. 2009 Jan 1. 457(7225):115-8.

    Insert

    Eriwinia chrysanthemi - pentameric ligand gated ion channel
    

    Use

    Tags

    His-10 and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 28, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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14. pET28a-HaloTag7SS-DogTag

    Plasmid

    #171775

    Purpose

    Expresses in bacterial cytoplasm HaloTag7 with DogTag inserted between D139 and E140, as well as C61S and C261S in HaloTag7 to block disulfide bond formation

    Depositor

    Mark Howarth

    Article

    Keeble et al Cell Chem Biol. 2021 Jul 28. pii: S2451-

    Insert

    HaloTag7SS-DogTag
    

    Use

    Tags

    His6

    Expression

    Bacterial

    Mutation

    DogTag inserted in HaloTag7 between residues D139…

    Promoter

    T7

    Available since

    July 29, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET28-2H12.D11

    Plasmid

    #221145

    Purpose

    His6-tagged cdGreen2 expressed from T7 promoter (Internal Lab ID: UJ11357)

    Depositor

    Urs Jenal

    Article

    Kaczmarczyk et al Nat Commun. 2024 May 9;15(1):3920. doi:

    Insert

    cdGreen2
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    June 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pET28a-SUMO-AsCas12f

    Plasmid

    #171612

    Purpose

    AsCas12f1 heterologous expression plasmid

    Depositor

    Quanjiang Ji

    Article

    Wu et al Nat Chem Biol. 2021 Sep 2. pii: 10.1038/

    Insert

    AsCas12f1
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Sept. 14, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pET28_human-pro-IL-18_DeltaN24

    Plasmid

    #214327

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    IL18 (IL18 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    Deletion of first 24 amino acids

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pET28_Caspase-5-enzymatic-domain

    Plasmid

    #214306

    Purpose

    Protein overexpression in bacteria

    Depositor

    Jonathan Kagan

    Article

    Devant et al Nature. 2023 Dec;624(7991):451-459. doi:

    Insert

    CASP5 (CASP5 Human)
    

    Use

    Tags

    His-TEV

    Expression

    Bacterial

    Mutation

    aa133-434

    Promoter

    T7

    Available since

    Feb. 21, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET21-pelB-LaM-2

    Plasmid

    #172768

    Purpose

    Bacterial expression of anti-mCherry nanobody LaM-2, with pelB leader and C-term free cysteine and 6xHIS tag.

    Depositor

    Michael Rout

    Article

    Fridy et al Nat Methods. 2014 Dec;11(12):1253-60. do

    Insert

    LaM-2
    

    Use

    Tags

    6xHIS and pelB leader for periplasmic secretion

    Expression

    Bacterial

    Mutation

    Promoter

    T7

    Available since

    Aug. 18, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pET22b-His6-UBCH7

    Plasmid

    #212711

    Purpose

    Bacterial expression of His6-UBCH7

    Depositor

    Helle Ulrich

    Article

    Renz et al Mol Cell. 2024 Jan 18;84(2):386-400.e11.

    Insert

    UBCH7
    

    Use

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    Promoter

    Available since

    Jan. 8, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge