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pCold I-Hero7

Plasmid

#187926

Purpose

Bacterial expression of heat-resistant obscure (Hero) protein Hero7

Depositor

Yukihide Tomari

Article

Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

Insert

Hero7 (SERF2 Human)

Tags

Flag-6xHis

Expression

Bacterial

Available Since

Oct. 17, 2022

Availability

Academic Institutions and Nonprofits only

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EGFR∆550-580-mEGFP

Plasmid

#203788

Purpose

Number and brightness analysis of EGFR oligomerization

Depositor

Thorsten Wohland

Article

Balasubramanian et al Biophys J. 2022 Dec 6;121(23):4452-4466.

Insert

EGFR (EGFR Human)

Tags

mEGFP

Expression

Mammalian

Mutation

aa 550-580 deleted

Available Since

July 27, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-2-Rhoc-m_3'UTR

Plasmid

#31885

Depositor

Robert Blelloch

Article

Subramanyam et al Nat Biotechnol. 2011 May;29(5):443-8. Ep

Insert

Rhoc mutant 3'UTR (RHOC Human)

Use

Luciferase

Tags

Luciferase

Expression

Mammalian

Mutation

miR-302b, 372 7-mer seed match mutated from AGCAC…

Promoter

SV40

Available Since

Sept. 14, 2011

Availability

Academic Institutions and Nonprofits only
YFP Gamma 10

Plasmid

#36108

Depositor

Narasimhan Gautam

Article

Saini et al J Biol Chem. 2007 Aug 17;282(33):24099-1

Insert

G protein subunit Gamma 10 (Gng10 Mouse)

Tags

YFP

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 6, 2012

Availability

Academic Institutions and Nonprofits only
pBX_RPS25_smRuby2_HA

Plasmid

#236090

Purpose

Constitutively encodes RPS25-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting RPS25 in the 40S subunit.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

RPS25-smRuby2-HA (RPS25 Synthetic, Human)

Expression

Mammalian

Available Since

April 8, 2025

Availability

Academic Institutions and Nonprofits only

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pCAGEN_NES_V5_GFP_FTA

Plasmid

#235633

Purpose

Encodes V5-GFP with an N-terminal nuclear export sequence and a C-terminal FLAG-TEV cleavage site-AviTag. Used to express a control substrate for ALIBi.

Depositor

Maria Barna

Article

Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

Insert

GFP

Tags

FLAG-TEV cleavage site-AviTag and NES-V5

Expression

Mammalian

Available Since

April 2, 2025

Availability

Academic Institutions and Nonprofits only

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pHPHS927

Plasmid

#228238

Purpose

Bxb1 attB_GA mCherry-T2A-puro, Dox-inducible β-globin reporter for integration into Bxb1 attP_GA landing pad

Depositor

Arvind Rasi Subramaniam

Article

Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

Inserts

mCherry
Beta globin

Tags

3xFLAG, HA, and PuroR

Expression

Mammalian

Promoter

TRE3GV and none

Available Since

Dec. 4, 2024

Availability

Academic Institutions and Nonprofits only

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pRB133.2

Plasmid

#181950

Purpose

aTc-inducible expression of PhoP with C-terminal mNeonGreen fusion. Also contains mCherry under PhoP-controlled promoter PvirK

Depositor

Jeffrey Tabor

Article

Butcher et al Proc Natl Acad Sci U S A. 2022 Aug 30;11

Insert

PhoP-Salmonella enterica subsp. enterica serovar Typhimurium (AX04_RS23485 Synthetic, PhoP-Salmonella enterica subsp. enterica serovar Typhimurium)

Tags

mNeonGreen

Expression

Bacterial

Promoter

PhoP-mNG-PLtetO-1; mCherry-PvirK; tetR-J23106

Available Since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(M1)-Fc-IG

Plasmid

#183723

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Mutation

R47Q

Available Since

Oct. 14, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(M2)-Fc-IG

Plasmid

#183724

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Mutation

A108V

Available Since

Sept. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pMXs-Sig10(DM)-Fc-IG

Plasmid

#183725

Purpose

Expresses the fusion protein containing the extracellular part of the human Siglec-10 R47Q A108V mutant and the Fc region of human IgG1, and GFP

Depositor

Takeshi Tsubata

Article

Alborzian Deh Sheikh et al J Autoimmun. 2021 Jan;116:102571. doi: 1

Insert

Siglec-10

Use

Retroviral

Tags

Fc of human IgG1

Mutation

R47Q and A108V

Available Since

June 7, 2022

Availability

Academic Institutions and Nonprofits only

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KG#797

Plasmid

#110916

Purpose

Expresses the active zone - enriched protein Sentryn-GFP in a subset of cholinergic ventral nerve cord motor neurons

Depositor

Kenneth Miller

Article

Edwards et al Genetics. 2018 Nov;210(3):947-968. doi:

Inserts

unc-129 promoter
STRN-1 (Sentryn)-GFP
unc-54 3' control region with 1 intron just upstream and 1 intron in control region

Tags

GFP (contains 3 artificial introns)

Expression

Bacterial

Available Since

March 11, 2021

Availability

Academic Institutions and Nonprofits only

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pNG1304

Plasmid

#162488

Purpose

Expresses HelD K239A from Bacillus subtillis

Depositor

Peter Lewis

Article

Newing et al Nat Commun. 2020 Dec 18;11(1):6420. doi:

Insert

HelD (helD Bacillus subtillis 168)

Tags

6His-V5

Expression

Bacterial

Mutation

Changed Lysine 239 to Alanine (K239A)

Available Since

Jan. 14, 2021

Availability

Academic Institutions and Nonprofits only

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pCR4-*mRFP

Plasmid

#47118

Purpose

Can be used to make an RNA probe for whole-mount in situ hybridization, for in-frame fusion proteins or as a subcloning step for mRFP. Note that the coding sequence for mRFP lacks the start codon.

Depositor

Stephen Ekker

Article

Clark et al Nat Methods. 2011 Jun;8(6):506-15. Epub

Insert

*mRFP

Expression

Bacterial

Mutation

No start codon

Promoter

Lac

Available Since

Oct. 16, 2013

Availability

Academic Institutions and Nonprofits only

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TAL3467

Plasmid

#47186

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-inhibitorofDNAbinding3-right (id3 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
TAL3466

Plasmid

#47185

Depositor

Keith Joung

Article

Sander et al Nat Biotechnol. 2011 Aug 5;29(8):697-8.

Insert

ZebrafishCommunity-inhibitorofDNAbinding3-left (id3 Zebrafish)

Use

T7

Tags

3X Flag and WT FOKI

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 13, 2013

Availability

Academic Institutions and Nonprofits only
pECE-M2-PPP1R12C wt

Plasmid

#31659

Depositor

Anne Brunet

Article

Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

Insert

Protein Phosphatase 1 Regulatory Subunit 12C (PPP1R12C Human)

Tags

M2

Expression

Mammalian

Mutation

codon-optimized

Available Since

April 11, 2012

Availability

Academic Institutions and Nonprofits only
pcDNA3.1(+)-FluoSTEP-AKAR

Plasmid

#181844

Purpose

Genetically encoded FRET-based sensor for monitoring PKA activity near a protein of interest. Must pair with GFP11-tagged POI to reconstitute donor fluorescence.

Depositor

Jin Zhang

Article

Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

Insert

FluoSTEP-AKAR

Tags

mRuby2 and sfGFP(1-10)

Expression

Mammalian

Promoter

CMV

Available Since

June 17, 2022

Availability

Academic Institutions and Nonprofits only

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pFudioFRTPSD-95TealW

Plasmid

#134299

Purpose

Expresses PSD95 fused to Teal fluorescent protein upon coexpression with the recombinase flippase (Flp)

Depositor

Elly Nedivi

Article

Subramanian et al Cell Rep. 2019 Aug 6;28(6):1584-1595.e5.

Insert

PSD-95-Teal (Dlg4 Rat, Mouse)

Use

Lentiviral; Flp/frt

Tags

Teal

Expression

Mammalian

Promoter

UbC

Available Since

Sept. 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLVX-EF1a-CCO-IRES-Puromycin

Plasmid

#134861

Purpose

Expresses EGFP-tagged inner mitochondrial membrane marker

Depositor

David Andrews

Article

Schormann et al J Cell Biol. 2020 Mar 2;219(3). pii: 133

Insert

cytochrome c oxidase subunit VIII (COX8A Human)

Use

Lentiviral

Tags

EGFP

Expression

Mammalian

Promoter

EF1a

Available Since

Jan. 26, 2021

Availability

Academic Institutions and Nonprofits only

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