We narrowed to 9,984 results for: SUB;

1. pHPHS800

   Plasmid

   #228240

   Purpose

   Bxb1 attB_wildtype site and SpCas9-hygro for integration into Bxb1 attP_wildtype landing pad (eg. pHPHS232). Includes pEF1 and attP_GA for second round of recombination

   Depositor

   Arvind Rasi Subramaniam

   Article

   Nugent et al Nat Methods. 2025 Jun;22(6):1237-1246. d

   Insert

   Cas9
   

   Tags

   Hygromycin phosphotransferase

   Expression

   Mammalian

   Promoter

   none

   Available Since

   Dec. 2, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. Matrix-roGFP

   Plasmid

   #49437

   Purpose

   Expresses thiol redox-sensitive ratiometric sensor roGFP in the mitochondrial matrix of mammalian cells

   Depositor

   Paul Schumacker

   Article

   Waypa et al Circ Res. 2010 Feb 19;106(3):526-35. doi

   Insert

   Matrix-roGFP2
   

   Use

   Adenoviral

   Tags

   Cytochrome c oxidase subunit IV mitochondrial tar…

   Expression

   Mammalian

   Mutation

   C48S, Q80R, S147C, and Q204C in Clontech's G…

   Promoter

   CMV

   Available Since

   Dec. 10, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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3. pcDNA3-EGFP-Rac1-Q61L

   Plasmid

   #12981

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 constitutively active (RAC1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Q61L Constitutively active

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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4. pcDNA3-EGFP-Rac1-T17N

   Plasmid

   #12982

   Depositor

   Gary Bokoch

   Article

   Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

   Insert

   Rac1 DN (RAC1 Human)
   

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   T17N Dominant negative

   Available Since

   Oct. 27, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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5. pBsVchCAST-pyrE

   Plasmid

   #203813

   Purpose

   Expression of CRISPR-associated transposases, shuttle vector for Bacillus subtilis / E. coli

   Depositor

   Sheng Yang

   Article

   Yang et al ACS Synth Biol. 2023 Jul 21;12(7):2198-2

   Insert

   VchTniQ, VchCas5/8, VchCas7, VchCas6, VchTnsABC, CRISPR(pyrE)
   

   Use

   CRISPR

   Expression

   Bacterial

   Available Since

   Aug. 11, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCold I-Hero45

   Plasmid

   #187931

   Purpose

   Bacterial expression of heat-resistant obscure (Hero) protein Hero45

   Depositor

   Yukihide Tomari

   Article

   Tsuboyama et al PLoS Biol. 2020 Mar 12;18(3):e3000632. d

   Insert

   Hero45 (SERBP1 Human)
   

   Tags

   Flag-6xHis

   Expression

   Bacterial

   Available Since

   Oct. 17, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pBABE puro mouse IRS-1 myc

   Plasmid

   #11374

   Depositor

   Ronald Kahn

   Article

   Tsuruzoe et al Mol Cell Biol. 2001 Jan . 21(1):26-38.

   Insert

   IRS-1 (Irs1 Mouse)
   

   Use

   Retroviral

   Tags

   myc

   Expression

   Mammalian

   Available Since

   April 20, 2006

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCDNA3_Sec61B-V5-APEX2

   Plasmid

   #83411

   Purpose

   Expressed C-term tagged APEX2 on Sec61B in mammalian cell

   Depositor

   Hyun-Woo Rhee

   Article

   Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

   Insert

   Sec61B-V5-APEX2 (SEC61B Synthetic, Human)
   

   Tags

   APEX2 and V5

   Expression

   Mammalian

   Promoter

   CMV-F

   Available Since

   March 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pBX_RPL7a_smRuby2_HA

   Plasmid

   #236087

   Purpose

   Constitutively encodes RPL7a-smRuby2-HA (mRuby2-based spaghetti monster fluorescent protein embedding HA epitope tags), for targeting the 60S subunit.

   Depositor

   Maria Barna

   Article

   Zhang et al Science. 2025 Mar 7;387(6738):eadn2623.

   Insert

   RPL7a-smRuby2-HA (RPL7A Synthetic, Human)
   

   Expression

   Mammalian

   Available Since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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10. pcDNA3-EGFP-Cdc42-wt

    Plasmid

    #12975

    Depositor

    Gary Bokoch

    Article

    Subauste et al J Biol Chem. 2000 Mar 31. 275(13):9725-3

    Insert

    Cdc42 (CDC42 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 27, 2006

    Availability

    Academic Institutions and Nonprofits only

    

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11. pWC2

    Plasmid

    #15960

    Depositor

    Jeff Gelles

    Article

    Subramanian et al J Gen Physiol. 2007 Nov . 130(5):445-55.

    Insert

    Kinesin 1-401 BCCP his tag (Khc E. coli, Fly)
    

    Tags

    BCCP and His6

    Expression

    Bacterial

    Mutation

    amino acids 1-401

    Available Since

    Nov. 27, 2007

    Availability

    Academic Institutions and Nonprofits only

    

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12. pEJS1084_pTetR-P2A-BFP/BspMI flexible sgRNA

    Plasmid

    #117687

    Purpose

    U6 driven Spy sgRNA cloning vector where guide sequences are inserted between BspMI sites. This plasmid works with Addgene #108570 for subnuclear proteomic profiling via C-BERST method.

    Depositor

    Erik Sontheimer

    Article

    Gao et al Methods Enzymol. 2019;616:365-383. doi:

    Inserts

    Kanamycin cassette
    TetR-P2A-BFP
    

    Use

    CRISPR and Lentiviral

    Expression

    Mammalian

    Promoter

    U6 and hPGK

    Available Since

    Dec. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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13. AP-1 mu1-GFP

    Plasmid

    #173432

    Purpose

    Expression of AP-1 Mu1 subunit with a terminal GFP

    Depositor

    Juan Bonifacino

    Article

    Guo et al Dev Cell. 2013 Nov 11;27(3):353-66. doi:

    Insert

    AP1M1 (Ap1m1 Mouse)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pECE-HA-AMPKbeta1 wt

    Plasmid

    #31666

    Depositor

    Anne Brunet

    Article

    Banko et al Mol Cell. 2011 Dec 23;44(6):878-92. Epub

    Insert

    protein kinase, AMP-activated, beta 1 non-catalytic subunit (PRKAB1 Human)
    

    Tags

    HA (YDVPDYASL)

    Expression

    Mammalian

    Available Since

    April 11, 2012

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLX307-VHL(Y98H)-3F-BirA

    Plasmid

    #220148

    Purpose

    Expresses VHL(Y98H)-BirA as a control for VHL substrate identification by E-STUB

    Depositor

    William Sellers

    Article

    Huang et al Nat Chem Biol. 2024 Mar 21. doi: 10.1038

    Insert

    von Hippel-Lindau tumor suppressor (VHL Human)
    

    Use

    Lentiviral

    Tags

    3F-BirA

    Expression

    Mammalian

    Mutation

    changed tyrosine 98 to histidine; codon optimized

    Promoter

    EF-1a

    Available Since

    May 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pSKC11 - UTRQ

    Plasmid

    #110490

    Purpose

    Monitor the expression of Luciferase driven by the NRAS 5'-UTR.

    Depositor

    Shankar Balasubramanian

    Article

    Kumari et al Nat Chem Biol. 2007 Apr;3(4):218-21. doi

    Insert

    NRAS 5'-UTR (bp 1-254) (NRAS Human)
    

    Use

    Luciferase

    Tags

    Firefly luciferase

    Promoter

    T7

    Available Since

    July 20, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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17. mTagBFP2-MAPTau-N-10

    Plasmid

    #55312

    Purpose

    Localization: Microtubules, Excitation: 399, Emission: 456

    Depositor

    Michael Davidson

    Article

    Subach et al PLoS One. 2011;6(12):e28674. Epub 2011 D

    Insert

    MAPTau (MAPT Human)
    

    Tags

    mTagBFP2

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Oct. 10, 2014

    Availability

    Industry, Academic Institutions, and Nonprofits

18. pCDNA3_V5-APEX2-Sec61B

    Plasmid

    #83412

    Purpose

    Expressed N-term tagged APEX2 on Sec61B in mammalian cell

    Depositor

    Hyun-Woo Rhee

    Article

    Lee et al Cell Rep. 2016 May 11. pii: S2211-1247(1

    Insert

    V5-APEX2-Sec61B (SEC61B Synthetic, Human)
    

    Tags

    APEX2 and V5

    Expression

    Mammalian

    Promoter

    CMV-F

    Available Since

    March 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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19. pMRXIP Lamp1-Venus

    Plasmid

    #89937

    Purpose

    Expresses Lamp1 tagged with Venus in mammalian cells

    Depositor

    Noboru Mizushima

    Article

    Tsuboyama et al Science. 2016 Nov 25;354(6315):1036-1041

    Insert

    rat Lamp1 (Lamp1 Rat)
    

    Use

    Retroviral

    Tags

    Venus

    Available Since

    Oct. 4, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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20. Cytosolic Fyn FRET Biosensor

    Plasmid

    #124654

    Purpose

    FRET biosensor. The highly sensitive biosensor has a mutated SH2 domain, ECFP and YPet

    Depositor

    Yingxiao Wang

    Article

    Ouyang et al ACS Sens. 2019 Jan 25;4(1):76-86. doi: 1

    Insert

    Synthetic gene containing ECFP-SH2 domain-substrate-linker-YPet
    

    Expression

    Mammalian

    Available Since

    Feb. 18, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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