We narrowed to 3,935 results for: gast

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KHBD00413

Plasmid

#34520

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8365 (E(spl)m8-HLH Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
Mad GSK3

Plasmid

#22995

Depositor

Edward De Robertis

Publication

Eivers et al PLoS One. 2009 . 4(8):e6543.

Insert

Mad (Mad Fly)

Tags

FLAG

Expression

Mammalian

Mutation

S208A, S204A

Available since

June 7, 2010

Availability

Academic Institutions and Nonprofits only
pRD60 (dE2F)

Plasmid

#14820

Depositor

Robert Duronio

Insert

E2F (E2f1 Fly)

Expression

Bacterial

Available since

July 30, 2007

Availability

Academic Institutions and Nonprofits only
pKC31

Plasmid

#226777

Purpose

Expresses Drosophila UNC-104(R562H) in insect cells

Depositor

Kyoko Chiba

Publication

Niwa et al J Cell Sci. 2024 Sep 6:jcs.262017. doi:

Insert

Drosophila unc-104 (R562H) (unc-104 Fly)

Tags

sfGFP and 2xStrepII

Expression

Insect

Mutation

changed Arginine 562 to Histidine (bris mutation)

Available since

Oct. 21, 2024

Availability

Academic Institutions and Nonprofits only

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pBS-KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Plasmid

#217512

Purpose

KD Recombinase dependent conditional smGdP-10XOllas cassette

Depositor

S. Lawrence Zipursky

Publication

Sanfilippo et al Neuron. 2024 Jan 13:S0896-6273(23)00973-

Insert

KDRT-STOP-loxP-3XP3::dsRed-loxP-STOP-KDRT-smGdP-10XOllas

Tags

smGdP-10XOllas

Expression

Insect

Available since

May 20, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-EGFP-DmNot1_J

Plasmid

#146709

Purpose

Insect Expression of DmNot1

Depositor

Elisa Izaurralde

Publication

Huntzinger et al Nucleic Acids Res. 2013 Jan;41(2):978-94

Insert

DmNot1 (Not1 Fly)

Expression

Insect

Mutation

Three non silent mutations N605S, K759M, G1999S a…

Available since

May 6, 2022

Availability

Academic Institutions and Nonprofits only

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pGEX-2T-TRR-C421-Y2404F

Plasmid

#182846

Purpose

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 2011-2431 aa). Expression in E. coli. by IPTG induction

Depositor

Peter Harte

Publication

Tie et al Development. 2014 Mar;141(5):1129-39. do

Insert

TRR 2011-2431 (trr Fly)

Expression

Bacterial

Mutation

mutation Y2404F (TAC/TTC) in GST-TRR-C421 (TRR 20…

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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6xHis(TEV)-dDrp1-StrepII

Plasmid

#174421

Purpose

Expresses D. melanogaster Drp1 in bacteria

Depositor

Thomas Pucadyil

Publication

Kamerkar et al Nat Commun. 2018 Dec 7;9(1):5239. doi: 1

Insert

Drp1 (Drp1 Fly)

Tags

6xHis(TEV) and StrepII

Expression

Bacterial

Mutation

None

Promoter

T7

Available since

Sept. 30, 2021

Availability

Academic Institutions and Nonprofits only

Cited by

1 Article

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Hy_pMT Flag-Hel25E K91N

Plasmid

#110121

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with K91N mutation that greatly reduces helicase activity

Depositor

Jeremy Wilusz

Publication

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

K91N mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Flag-Hel25E D193E

Plasmid

#110120

Purpose

Expresses Flag-tagged D. melanogaster Hel25E with D193E mutation that greatly reduces ATP binding affinity

Depositor

Jeremy Wilusz

Publication

Huang et al Genes Dev. 2018 May 17. pii: gad.314856.

Insert

Hel25E (Hel25E Fly)

Tags

Flag

Expression

Insect

Mutation

D193E mutation

Promoter

Metallothionein Promoter (pMT)

Available since

Sept. 27, 2020

Availability

Academic Institutions and Nonprofits only

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Hy_pMT Laccase2 MCS-HIPK3 Exon

Plasmid

#69888

Purpose

Expresses the human HIPK3 exon 2 circular RNA in Drosophila

Depositor

Jeremy Wilusz

Publication

Kramer et al Genes Dev. 2015 Oct 8.

Insert

HIPK3 (HIPK3 Human, Fly)

Expression

Insect

Mutation

Expresses aa1-194 of HIPK3

Promoter

Metallothionein Promoter (pMT)

Available since

Nov. 11, 2015

Availability

Academic Institutions and Nonprofits only

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pcDNA3.2 mir-1-1 reporter dme-mir-125

Plasmid

#46667

Depositor

David Bartel

Publication

Auyeung et al Cell. 2013 Feb 14;152(4):844-58. doi: 10

Insert

dme-mir-125

Use

RNAi

Tags

V5 and hsa-mir-1-1

Expression

Mammalian

Promoter

CMV

Available since

June 26, 2015

Availability

Academic Institutions and Nonprofits only

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pTTR210K

Plasmid

#41750

Depositor

Jeff Gelles

Publication

Thoresen et al Biochemistry. 2008 Sep 9;47(36):9514-21.

Insert

kinesin heavy chain (Khc E. coli, Fly)

Tags

His6 and bccp

Expression

Bacterial

Mutation

residues 1-401; R210K

Available since

July 29, 2013

Availability

Academic Institutions and Nonprofits only
pUASp YFP Rab32 CA

Plasmid

#37740

Depositor

Matthew Scott

Publication

Zhang et al Genetics. 2007 Jun;176(2):1307-22. Epub

Insert

Rab32 (Rab32 Fly)

Use

Drosophila

Tags

YFP

Expression

Insect

Mutation

Q79L (constitutive active)

Promoter

GAL4

Available since

Nov. 20, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00624

Plasmid

#39696

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG7450 (CrebA Fly)

Use

Gateway Cloning

Promoter

none

Available since

Aug. 28, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00510

Plasmid

#39611

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG8365 (E(spl)m8-HLH Fly)

Use

Gateway Cloning

Promoter

none

Available since

Aug. 20, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00070

Plasmid

#34228

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG10325 (abd-A Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
KHBD00009

Plasmid

#34172

Depositor

Bart Deplancke

Publication

Korneel et al Nat Methods. 2011 Oct 30;8(12):1065-70.

Insert

CG5058 (grh Fly)

Use

Gateway Cloning

Available since

Jan. 23, 2012

Availability

Academic Institutions and Nonprofits only
pAc5.1-EGFP-Vasa 463-661 MUT (F504E) (HK196)

Plasmid

#206434

Purpose

Vasa 463-661 expression in Schneider cells for imaging

Depositor

Mandy Jeske

Publication

Salgania et al Nat Commun. 2024 Apr 3;15(1):2875. doi:

Insert

Vasa 463-661 (vas Fly)

Tags

EGFP

Expression

Insect

Mutation

F504E

Available since

July 30, 2024

Availability

Academic Institutions and Nonprofits only

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pAc5.1B-lambdaN-HA-DmeIF4G-trunc-V5His6_G

Plasmid

#146392

Purpose

Insect Expression of DmeIF4G-trunc. *Note: this plasmid does not contain an HA-tag as the name implies.

Depositor

Elisa Izaurralde

Publication

Zekri et al Mol Cell Biol. 2009 Dec;29(23):6220-31.

Insert

DmeIF4G-trunc (eIF4G1 Fly)

Expression

Insect

Mutation

322-1666 truncated version of elF4G sequence with…

Available since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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