We narrowed to 17,615 results for: jun

1. 3xFlag-eGFP-Flag-SETX

   Plasmid

   #218838

   Purpose

   Mammalian expression of GFP-tagged SETX construct for subcellular localization studies with GFP, or immunoprecipitation with Flag tag.

   Depositor

   Albert La Spada

   Article

   Bennett et al Heliyon. 2020 Jun 13;6(6):e04165. doi: 1

   Insert

   Senataxin (SETX Human)
   

   Use

   Tags

   Flag and eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pENN496_miniCRISPRcharm_dSaCas9

   Plasmid

   #220837

   Purpose

   mini CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   mini CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   EFS

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. pENN239_CRISPRcharm_Kv1

   Plasmid

   #220838

   Purpose

   CRISPRcharm Kv1 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv1
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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4. pENN236_CRISPRcharm_Kv2

   Plasmid

   #220839

   Purpose

   CRISPRcharm Kv2 expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm Kv2
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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5. OPEN-A*24:02

   Plasmid

   #235133

   Purpose

   E. coli expression of HLA-A*24:02 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*24:02 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. OPEN-HLA-A*01:01

   Plasmid

   #235134

   Purpose

   E. coli expression of HLA-A*01:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*01:01 (HLA-A Human)
   

   Use

   Tags

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 28, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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7. OPEN-HLA-A*11:01-BirA

   Plasmid

   #235135

   Purpose

   E. coli expression of BirA tagged HLA-A*11:01 containing a cysteine mutation for disulfide linkage to mutant beta-2 microglobulin.

   Depositor

   Nikolaos Sgourakis

   Article

   Sun et al Proc Natl Acad Sci U S A. 2023 Jun 20;12

   Insert

   OPEN-HLA-A*11:01 (HLA-A Human)
   

   Use

   Tags

   BirA

   Expression

   Bacterial

   Mutation

   G120C

   Promoter

   Available since

   April 8, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pENN226_CRISPRcharm

   Plasmid

   #220834

   Purpose

   CRISPRcharm expression in mammalian cells

   Depositor

   Jonathan Weissman

   Article

   Neumann et al Science. 2024 Jun 28;384(6703):ado7082.

   Insert

   CRISPRcharm
   

   Use

   CRISPR

   Tags

   P2A-TagBFP

   Expression

   Mammalian

   Mutation

   N/A

   Promoter

   CAG

   Available since

   June 25, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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9. PCBP1_Full-length_pGEX

   Plasmid

   #135108

   Purpose

   Recombinant expression of PCBP1 with dual-affinity tag

   Depositor

   Christopher Burge

   Article

   Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

   Insert

   PCBP1 (PCBP1 Human)
   

   Use

   Tags

   GST-SBP

   Expression

   Bacterial

   Mutation

   Promoter

   tac

   Available since

   Nov. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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10. IGF2BP1_2xRRM-4xKH_pGEX

    Plasmid

    #135122

    Purpose

    Recombinant expression of IGF2BP1 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    IGF2BP1 (IGF2BP1 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 30, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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11. pX458-Dual-Guide-Donor-ShuttleOnly

    Plasmid

    #171098

    Purpose

    Donor shuttle plasmid to enable ligation of a second U6-GuideRNA casssette into any pX458 family backbone via digestion of both plasmids with XbaI and KpnI.

    Depositor

    Joseph Brzezinski

    Article

    Kaufman et al Development. 2021 Jun 15;148(12). pii: 2

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mutation

    XbaI site added upstream of U6 promotor. GtoC mu…

    Promoter

    Available since

    Aug. 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA4/TO-Flag-H3.3

    Plasmid

    #47980

    Purpose

    tetracycline-inducible expression of human Flag tagged H3F3A

    Depositor

    Bing Zhu

    Article

    Huang et al PLoS Genet. 2013 Jun;9(6):e1003558. doi:

    Insert

    H3F3A (H3-3A Human)
    

    Use

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    288 bp T to C synonymous mutation

    Promoter

    CMV promoter with tetracycline operator

    Available since

    Sept. 5, 2013

    Availability

    Academic Institutions and Nonprofits only

13. pLenti-Ef1a-hMGT-PGK-puro

    Plasmid

    #172394

    Purpose

    Lentiviral expression vector for human direct cardiac reprogramming

    Depositor

    Li Qian

    Article

    Garbutt et al STAR Protoc. 2020 Jun 19;1(1). doi: 10.1

    Insert

    Mef2c, Gata4, Tbx5 (MEF2C, GATA4, TBX5 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    Promoter

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pES001 MBP-GSK3β-HA-His

    Plasmid

    #154010

    Purpose

    Expresses MBP-GSK3β-HA-His (human Glycogen Synthase Kinase 3β as a fusion protein with MBP, HA, and His- tags) in E.coli

    Depositor

    Jesse Zalatan

    Article

    Gavagan et al Cell Syst. 2020 Jun 24;10(6):515-525.e5.

    Insert

    GSK3B (GSK3B Human)
    

    Use

    Tags

    HA tag, His6, and MBP

    Expression

    Bacterial

    Mutation

    Promoter

    Tac

    Available since

    Jan. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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15. pLL3.7-EF-EYFP-YAP1_WT-PolyA

    Plasmid

    #112284

    Purpose

    Fluorescently labels WT human YAP1-2gamma isoform (504 aa)

    Depositor

    Erik Sahai

    Article

    Ege et al Cell Syst. 2018 Jun 27;6(6):692-708.e13.

    Insert

    EYFP-YAP1 (YAP1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mutation

    WT human YAP1-2gamma

    Promoter

    EF-1 alpha

    Available since

    May 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pLenti_Cas9_T2A_mNeonGreen_P2A_blasticidin

    Plasmid

    #211471

    Purpose

    lentiviral tricistronic complex expressing Cas9, mNeonGreen, and blasticidin resistance

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    Cas9_T2A_mNeonGreen_P2A_BlasticidinR
    

    Use

    CRISPR and Lentiviral

    Tags

    3xFlag

    Expression

    Mammalian

    Mutation

    Promoter

    hU6, EF-1a

    Available since

    March 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. ILF2_DZF_pGEX

    Plasmid

    #135162

    Purpose

    Recombinant expression of ILF2 with dual-affinity tag

    Depositor

    Christopher Burge

    Article

    Dominguez et al Mol Cell. 2018 Jun 7;70(5):854-867.e9. d

    Insert

    ILF2 (ILF2 Human)
    

    Use

    Tags

    GST-SBP

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available since

    Nov. 14, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pLenti-dCas9-T2A-GFP

    Plasmid

    #193781

    Purpose

    Tricistronic expression of dCas9-EGFP-BSD

    Depositor

    James Eshleman

    Article

    Teh et al NAR Cancer. 2024 Jun 19;6(2):zcae028. do

    Insert

    dead Cas9
    

    Use

    Lentiviral

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    In frame with existing Cas9 promoter

    Available since

    May 28, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pHD-T2A-split-intein-Gal4[1-20]-N-int

    Plasmid

    #199203

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[1-20]N-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[1-20]-N-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pHD-T2A-split-intein-Gal4[21-881]-C-int

    Plasmid

    #199204

    Purpose

    To create "long homology arm" (~1000bp) knock-in donor vectors for split-intein Gal4[21-881]C-int

    Depositor

    Norbert Perrimon

    Article

    Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

    Insert

    T2A-split-intein-Gal4[21-881]-C-int
    

    Use

    Tags

    Expression

    Insect

    Mutation

    Promoter

    Available since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge