We narrowed to 5,532 results for: pcas

1. pCAGGS-FLAG-BMAL1

   Plasmid

   #186829

   Purpose

   Expresses FLAG-BMAL1 in mammalian cells

   Depositor

   Hung-Chun Chang

   Article

   Li et al Commun Biol. 2022 Jul 28;5(1):750. doi:

   Insert

   bmal1 (Bmal1 Mouse)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 3, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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2. pX551-miniCMV-SpCas9

   Plasmid

   #107031

   Purpose

   AAV plasmid expressing Cas9 under control of miniCMV promoter

   Depositor

   Alex Hewitt

   Insert

   SpCas9
   

   Use

   AAV and CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 18, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pX330_DRS-1 sgRNA / hSpCas9

   Plasmid

   #172841

   Purpose

   Mammalian expression of the DRS-1 synthetic sgRNA sequence under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   DRS-1 sgRNA under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-SRGAP2A-EGFP

   Plasmid

   #168501

   Purpose

   Expression of full length SRGAP2 with a C-termonal EGFP fusion

   Depositor

   Franck Polleux

   Article

   Guerrier et al Cell. 2009 Sep 4;138(5):990-1004. doi: 1

   Insert

   SRGAP2A (SRGAP2 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG-EGFP-C-IFT88

   Plasmid

   #218733

   Purpose

   Expresses N-terminally EGFP-tagged IFT88 in mammalian cells

   Depositor

   Kazuhisa Nakayama

   Article

   Ishida et al Mol Biol Cell. 2022 Aug 1;33(9):ar83. do

   Insert

   IFT88 (IFT88 Human)
   

   Use

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 22, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pX330_VCL sgRNA / hSpCas9

   Plasmid

   #172837

   Purpose

   Mammalian expression of a sgRNA targeting the intron 21 (last intron) of VCL (Zhong et al, eLife 2021) under the U6 promotor and hSpCas9 under the CAG promotor. This construct is based on pX330.

   Depositor

   Haining Zhong

   Article

   Zhong et al Elife. 2021 Jun 8;10. pii: 64911. doi: 1

   Insert

   sgRNA targeting intron 21 of VCL under the U6 promotor and hSpCas9 under the CAG promotor
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. Tubp-Gal80 in pCaSpeR4

   Plasmid

   #17748

   Depositor

   Liqun Luo

   Article

   Lee et al Neuron. 1999 Mar . 22(3):451-61.

   Insert

   GAL80 (GAL80 Budding Yeast)
   

   Use

   Tags

   SV40 polyA and Tubulin 1alpha promoter

   Expression

   Insect

   Mutation

   Promoter

   Available since

   May 14, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pCAX-mScarlet-CIP4

   Plasmid

   #178364

   Purpose

   Expresses CIP4 c-terminal labeled with mScarlet in mammalian cells

   Depositor

   Erik Dent

   Article

   Taylor et al Life Sci Alliance. 2019 Jun 3;2(3). pii:

   Insert

   Cdc42 Interacting Protein 4 (TRIP10 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Jan. 11, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG mEmerald-LAMP1

   Plasmid

   #168513

   Purpose

   Expression of rat LAMP1 fused with mEmerald to perform live tracking of lysosomes

   Depositor

   Franck Polleux

   Article

   Courchet et al Cell. 2013 Jun 20;153(7):1510-25. doi: 1

   Insert

   LAMP1 (Lamp1 Rat)
   

   Use

   Tags

   mEmerald

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 20, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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10. DHFR-SpCas9-DHFR

    Plasmid

    #85447

    Purpose

    Expresses SpCas9 fused to DHFR domain on both N- and C-termini in mammalian cells

    Depositor

    Amit Choudhary

    Article

    Maji et al Nat Chem Biol. 2017 Jan;13(1):9-11. doi:

    Insert

    DHFR-SpCas9-DHFR
    

    Use

    AAV

    Tags

    Destabilized domain (C-terminus version) of E.col…

    Expression

    Mammalian

    Mutation

    Promoter

    Cbh

    Available since

    Feb. 9, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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11. pAAV-pCaMKII-tTAc

    Plasmid

    #172125

    Purpose

    Expression of InteinC-tTAC in CaMKII positive cells

    Depositor

    Chun Xu

    Article

    Chen et al Nat Commun. 2022 Jun 23;13(1):3605. doi:

    Insert

    pCaMKII, tTAc
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    CaMKII

    Available since

    Sept. 24, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCAG/ATP11A(E186Q)-HA

    Plasmid

    #209249

    Purpose

    Mammalian expression of ATP11A mutant E186Q

    Depositor

    Hye-Won Shin

    Article

    Takatsu et al J Biol Chem. 2014 Nov 28;289(48):33543-5

    Insert

    ATP11A (ATP11A Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    E186Q

    Promoter

    Available since

    Nov. 7, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAGGS-ChrimsonR-mRuby2-ST

    Plasmid

    #105447

    Purpose

    Cation channelrhodopsin ChrimsonR fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites

    Depositor

    Hillel Adesnik

    Article

    Pegard et al Nat Commun. 2017 Oct 31;8(1):1228. doi:

    Insert

    ChrimsonR-mRuby2-ST
    

    Use

    Tags

    mRuby2 and soma targeting motif

    Expression

    Mammalian

    Mutation

    K176R in Chrimson

    Promoter

    pCAGGS

    Available since

    Feb. 15, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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14. pCAG-HA-Nrxn1beta AS4(+)

    Plasmid

    #59410

    Purpose

    Expression plasmid for HA-tagged mouse Neurexin 1beta AS4(+) [containing the insert at alternatively spliced segment 4 (AS4+)]

    Depositor

    Peter Scheiffele

    Article

    Chih et al Neuron. 2006 Jul 20. 51(2):171-8.

    Insert

    Nrxn1beta AS4(+) (Nrxn1 Mouse)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    June 11, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-hPPP3CA-FLAG

    Plasmid

    #179134

    Purpose

    Expression vector of human protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA) tagged with FLAG at C-terminus, CAG promoter, rabbit globin poly(A) signal.

    Depositor

    Masahito Ikawa

    Article

    Miyata et al Proc Natl Acad Sci U S A. 2021 Aug 31;11

    Insert

    protein phosphatase 3, catalytic subunit, alpha isoform (PPP3CA Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Jan. 25, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCAGGS puro HA-hUSP27X

    Plasmid

    #225718

    Purpose

    Bacterial expression of USP27X with HA tag

    Depositor

    Francisco Bustos

    Article

    Koch et al Life Sci Alliance. 2024 Jan 5;7(3):e2023

    Insert

    USP27X (USP27X Human)
    

    Use

    Tags

    HA

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 18, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pCAG-ChroME-mRuby2-ST

    Plasmid

    #108902

    Purpose

    Cation channelrhodopsin ChroME fused to mRuby2 fluorophore and targeted to the neuronal soma and proximal dendrites for optogenetic excitation

    Depositor

    Hillel Adesnik

    Article

    Mardinly et al Nat Neurosci. 2018 Apr 30. pii: 10.1038/

    Insert

    ChroME-mRuby2-ST
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CAG

    Available since

    May 9, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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18. modified pcAT7-Glo1

    Plasmid

    #160996

    Purpose

    Splicing reporter backbone

    Depositor

    Brenton Graveley

    Article

    Adamson et al Genome Biol. 2018 Jun 1;19(1):71. doi: 1

    Insert

    hemoglobin subunit beta (HBB Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Repeated intron 1 with 40 nucelotide deletion

    Promoter

    CAG

    Available since

    Jan. 13, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAS9-mCherry empty

    Plasmid

    #80975

    Purpose

    Cas9, mCherry, and sgRNA expression

    Depositor

    Veit Hornung

    Article

    Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

    Type

    Empty backbone

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Sept. 23, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330-SpCas9-HF1

    Plasmid

    #108301

    Purpose

    pX330 (Plasmid #42230) with the N497A, R661A, Q695A, and Q926A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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