We narrowed to 5,840 results for: pcas

1. SpCas9-ABE8e(V106W)

   Plasmid

   #198547

   Purpose

   Expresses human codon-optimized SpCas9-ABE8e(V106W) and blasticidin resistance: EFS promoter-SpCas9-ABE8e(V106W)-NLS-FLAG-P2A-BSD

   Depositor

   Hyongbum Kim

   Article

   Kim et al Nat Biotechnol. 2024 Mar;42(3):484-497.

   Insert

   SpCas9-ABE8e(V106W)
   

   Use

   CRISPR and Lentiviral

   Tags

   BSD, FLAG, and NLS

   Expression

   Mammalian

   Promoter

   EFS

   Available Since

   Nov. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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2. pCAG_Xph18-eGFP-CCR5TC

   Plasmid

   #135529

   Purpose

   Encodes a specific PSD-95 binder (Xph18) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph18
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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3. pCAS9-mCherry empty

   Plasmid

   #80975

   Purpose

   Cas9, mCherry, and sgRNA expression

   Depositor

   Veit Hornung

   Article

   Schmid-Burgk et al Nat Commun. 2016 Jul 28;7:12338. doi: 10

   Type

   Empty backbone

   Expression

   Mammalian

   Available Since

   Sept. 23, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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4. pCAG-Cre-iBSD

   Plasmid

   #140284

   Purpose

   Expression of Cre-IRES-BSD

   Depositor

   Yusuke Miyanari

   Article

   Kurihara et al Mol Cell. 2020 May 7;78(3):493-505.e8. d

   Insert

   Cre
   

   Expression

   Mammalian

   Available Since

   May 1, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCAG_Xph15-eGFP-CCR5TC

   Plasmid

   #135528

   Purpose

   Encodes a specific PSD-95 binder (Xph15) fused to eGFP, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph15
   

   Tags

   eGFP

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCAG_Xph20-mRuby2-CCR5TC

   Plasmid

   #135531

   Purpose

   Encodes a specific PSD-95 binder (Xph20) fused to mRuby2, CCR5 left-handed zinc finger, and KRAB(A) transcriptional repressor domain

   Depositor

   Matthieu Sainlos

   Article

   Rimbault et al Elife. 2024 Jan 3;13:e69620. doi: 10.755

   Insert

   Xph20
   

   Tags

   mRuby2

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   April 9, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-dGBP1-TagBFP

   Plasmid

   #80086

   Purpose

   GFP-dependent dGBP1-TagBFP, for expression in mammalian cells

   Depositor

   Connie Cepko

   Article

   Tang et al Elife. 2016 May 20;5. pii: e15312. doi:

   Insert

   dGBP1-TagBFP
   

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Dec. 1, 2016

   Availability

   Academic Institutions and Nonprofits only

   

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8. pX330 spCas9-mSA

   Plasmid

   #113096

   Purpose

   pX330 vector carrying spCas9-mSA for gene editing in mammalian cell lines

   Depositor

   Janet Rossant

   Article

   Gu et al Nat Biotechnol. 2018 Jun 11. pii: nbt.41

   Insert

   CAS9-MSA
   

   Use

   Synthetic Biology

   Tags

   MSA (monomeric streptavidin

   Mutation

   none

   Available Since

   Aug. 6, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSpCas9(BB)-2A-Puro-SMCR8

   Plasmid

   #83440

   Purpose

   Expresses Cas9 and a gRNA targeting SMCR8

   Depositor

   Shawn Ferguson

   Article

   Amick et al Mol Biol Cell. 2016 Aug 24. pii: mbc.E16

   Insert

   gRNA targeting human SMCR8 (SMCR8 Human)
   

   Expression

   Mammalian

   Promoter

   U6

   Available Since

   July 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCAX APP AENATA

    Plasmid

    #30144

    Depositor

    Tracy Young-Pearse, Dennis Selkoe

    Article

    Young-Pearse et al J Neurosci. 2007 Dec 26. 27(52):14459-69

    Insert

    Amyloid Precursor Protein (APP Human)
    

    Expression

    Mammalian

    Mutation

    YENPTY-->AENATA

    Available Since

    July 29, 2011

    Availability

    Academic Institutions and Nonprofits only

11. pX459V2.0-eSpCas9(1.1)

    Plasmid

    #108292

    Purpose

    pX459 V2.0 (Plasmid #62988) with the K848A, K1003A, and R1060A mutations

    Depositor

    Yuichiro Miyaoka

    Article

    Kato-Inui et al Nucleic Acids Res. 2018 May 18;46(9):467

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    May 21, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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12. pET28a SpyCatcher-SnoopCatcher

    Plasmid

    #72324

    Purpose

    Bacterial expression of SpyCatcher linked to SnoopCatcher.

    Depositor

    Mark Howarth

    Article

    Veggiani et al Proc Natl Acad Sci U S A. 2016 Jan 19. p

    Insert

    pET28a SpyCatcher-SnoopCatcher
    

    Tags

    N-terminal His6 tag

    Expression

    Bacterial

    Mutation

    35 amino acids at the N-term of SpyCatcher have b…

    Available Since

    Feb. 12, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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13. pCAG-EGFP-C-IFT172

    Plasmid

    #218734

    Purpose

    Expresses N-terminally EGFP-tagged IFT172 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Insert

    IFT172 (IFT172 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pX551-miniCMV-SpCas9

    Plasmid

    #107031

    Purpose

    AAV plasmid expressing Cas9 under control of miniCMV promoter

    Depositor

    Alex Hewitt

    Insert

    SpCas9
    

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    April 18, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCA528-PPxY1-GS-WW3e

    Plasmid

    #166705

    Purpose

    expresses an N-terminal fusion of AMOT PPxY1 to NEDD4L WW3 in bacterial cells

    Depositor

    Wesley Sundquist

    Article

    Rheinemann et al J Biol Chem. 2021 Jul 17:100975. doi: 10

    Insert

    AMOT PPxY1 fused to NEDD4L WW3
    

    Tags

    His-SUMO

    Expression

    Bacterial

    Promoter

    T7 promoter

    Available Since

    July 23, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRha-ABE7.10-SpCas9-NG

    Plasmid

    #170663

    Purpose

    Expresses the base editor ABE7.10-SpCas9-NG in bacterial cells

    Depositor

    David Liu

    Article

    Krishnamurthy et al Nucleic Acids Res. 2021 Oct 11;49(18):10

    Insert

    ABE7.10-SpCas9-NG
    

    Tags

    8xHIS and BP-NLS

    Expression

    Bacterial

    Promoter

    pRhaBAD

    Available Since

    Oct. 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pCA24N-sso7d-ligase

    Plasmid

    #87745

    Purpose

    IPTG-inducible expression of sso7d-ligase fusion for protein purification

    Depositor

    Wayne Patrick

    Article

    Wilson et al Protein Eng Des Sel. 2013 Jul;26(7):471-

    Insert

    T4 DNA ligase (30 )
    

    Tags

    6xHis and codon optimized sso7d (Sulfolobus solfa…

    Expression

    Bacterial

    Promoter

    T5-lac

    Available Since

    March 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. GFP Cav2.2 HA pCAGGS

    Plasmid

    #206054

    Purpose

    expression of rabbit Cav2.2 calcium channel with a N-terminal GFP tag and an exofacial double HA tag in domain II

    Depositor

    Annette Dolphin

    Article

    Macabuag et al J Neurosci. 2015 Oct 28;35(43):14636-52.

    Insert

    cacna1b (CACNA1B Rabbit)
    

    Tags

    GFP

    Expression

    Mammalian

    Promoter

    CMV/B-actin

    Available Since

    Oct. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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19. pCAIP-hXRCC1wt-eGFP

    Plasmid

    #206032

    Purpose

    Mammalian expression of hXRCC1wt coupled to eGFP under the control of a CAG promoter

    Depositor

    Primo Schaer

    Article

    Schwarz et al Nat Commun. 2024 Jan 2;15(1):184. doi: 1

    Insert

    hXRCC1
    

    Tags

    eGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCAGGS-PHFF-sh-hBVRA

    Plasmid

    #100285

    Purpose

    Expression vector for phycocyanobilin (PCB) synthesis and shRNA for human BVRA gene in mammalian cells

    Depositor

    Kazuhiro Aoki

    Article

    Uda et al Proc Natl Acad Sci U S A. 2017 Oct 24. p

    Inserts

    MTS-PcyA-FLAG-P2A-MTS-HA-HO1-P2A-MTS-Myc-Fd-P2A-MTS-Fnr-T7 (synthetic genes)
    shRNA for human BVRA
    

    Tags

    FLAG, T7 and Mitochondria-targeting sequence (MTS…

    Expression

    Mammalian

    Mutation

    All genes are codon-optimized for expression in h…

    Promoter

    CAG promoter and H1 promoter

    Available Since

    Sept. 20, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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