We narrowed to 2,375 results for: pcas9

1. pRDA_152

   Plasmid

   #179085

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   eCas9-1.1
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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2. pRDA_151

   Plasmid

   #179084

   Purpose

   nuclease Cas expression

   Depositor

   John Doench, David Root

   Article

   Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

   Insert

   Cas9-HF
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mutation

   Promoter

   Available Since

   Jan. 19, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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3. pYPQ159

   Plasmid

   #69308

   Purpose

   Gateway entry vector with hSpCas9D10A

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9D10A (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   D10A mutation; NLS fusion

   Promoter

   Available Since

   Nov. 4, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pX330-H11r1-2-DD-Cas9

   Plasmid

   #85978

   Purpose

   Expresses DD-SpCas9 in mammalian cells and targets the H11 locus in human cells

   Depositor

   Michele Calos

   Article

   Geisinger et al Nucleic Acids Res. 2016 Jan 13. pii: gkv

   Insert

   DD-SpCas9
   

   Use

   CRISPR

   Tags

   FKBP12 L106P DD

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   Aug. 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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5. pYPQ158

   Plasmid

   #69307

   Purpose

   Gateway entry vector with hSpCas9 (Human codon optimized)

   Depositor

   Yiping Qi

   Article

   Lowder et al Plant Physiol. 2015 Aug 21. pii: pp.0063

   Insert

   hSpCas9 (Human codon optimized)
   

   Use

   CRISPR

   Tags

   3XFLAG

   Expression

   Mutation

   NLS fusion

   Promoter

   Available Since

   Oct. 28, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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6. pSL1103

   Plasmid

   #129526

   Purpose

   pDEF-SpCas9, No Target Control Vector for CRISPRi, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available Since

   Sept. 10, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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7. pFH99

   Plasmid

   #128204

   Purpose

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   classic sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. pFH100

   Plasmid

   #128205

   Purpose

   improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)

   Depositor

   Vladimir Nekrasov

   Article

   Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

   Insert

   improved sgRNA backbone for SpCas9, combine with AtU6-26 promoter module (pFH35)
   

   Use

   CRISPR and Synthetic Biology

   Tags

   Expression

   Plant

   Mutation

   Promoter

   Available Since

   Aug. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pSL1315

   Plasmid

   #129527

   Purpose

   pDEF-SpCas9, Best (-458) Vector for CRISPRi of PyALBA4, PbEF1a promoter, 1st Generation

   Depositor

   Scott Lindner

   Article

   Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

   Insert

   dSpCas9
   

   Use

   Plasmodium, rodent-infectious species

   Tags

   NLS, 1xHA

   Expression

   Mutation

   Promoter

   Available Since

   Oct. 8, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pYPQAT02

    Plasmid

    #223374

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by AtU3 promoter; BASTA for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-AtU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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11. pYPQAT04

    Plasmid

    #223376

    Purpose

    T-DNA vector for SpCas9 mediated mutagenesis for monocot plants; NGG PAM; Cas9 was driven by ZmUbi1 and the sgRNA was driven by OsU3 promoter; Hygromycin for plants selection.

    Depositor

    Yiping Qi

    Article

    Cheng et al Plant Cell. 2025 Apr 9:koaf081. doi: 10.

    Insert

    ZmUbi-SpCas9-OsU3-gRNA scaffold
    

    Use

    CRISPR

    Tags

    3x FLAG and NLS

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    April 10, 2025

    Availability

    Academic Institutions and Nonprofits only

    

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12. pSK-Spsg

    Plasmid

    #214351

    Purpose

    Expresses cloning backbone for SpCas9 sgRNA

    Depositor

    Yongming Wang

    Article

    Qi et al Nat Chem Biol. 2023 Dec 5. doi: 10.1038/

    Insert

    SpCas9 tracrRNA
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Feb. 29, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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13. pSL1211

    Plasmid

    #129521

    Purpose

    pDEF-SpCas9::GFP, For deletion of pyalba4, PyGAPDH promoter, 2nd Generation

    Depositor

    Scott Lindner

    Article

    Walker et al J Biol Chem. 2019 Jun 14;294(24):9555-95

    Insert

    SpCas9
    

    Use

    Plasmodium, rodent-infectious species

    Tags

    3XFlag-NLS

    Expression

    Mutation

    Promoter

    Available Since

    Sept. 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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14. pFH70

    Plasmid

    #128190

    Purpose

    Level1 ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt

    Depositor

    Vladimir Nekrasov

    Article

    Hahn et al BMC Plant Biol. 2020 Apr 23;20(1):179. d

    Insert

    ZmUBIp::nSpCas9-PmCDA (Arabidopsis codon optimized):NOSt
    

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Plant

    Mutation

    Promoter

    Available Since

    Aug. 19, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pEPOR0SP0014

    Plasmid

    #117524

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-KA

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-KA (with stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    K855A

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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16. pEPOR0SP0009

    Plasmid

    #117522

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-p (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-p (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 13, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pICSL90005

    Plasmid

    #117520

    Purpose

    Level0 Golden Gate part: CDS, SpCas9-h (no stop codon)

    Depositor

    Nicola Patron

    Article

    Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

    Insert

    SpCas9-h (no stop codon)
    

    Use

    CRISPR

    Tags

    Expression

    Mutation

    Promoter

    Available Since

    Nov. 8, 2018

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pX330A-1x2

    Plasmid

    #58766

    Purpose

    Expresses Cas9 nuclease and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 nuclease
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. pNuc-cis

    Plasmid

    #202796

    Purpose

    RK2-based conjugative plasmid containing the TevSpCas9 dual-endonuclease for targeted bacterial killing

    Depositor

    David Edgell

    Article

    Hamilton et al Nat Commun. 2019 Oct 4;10(1):4544. doi:

    Insert

    TevSpCas9, sgRNA cassette, oriT, CenArsHis
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Promoter

    Available Since

    Aug. 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. pX330A_D10A-1x2

    Plasmid

    #58772

    Purpose

    Expresses Cas9 nickase and gRNA

    Depositor

    Takashi Yamamoto

    Article

    Sakuma et al Sci Rep. 2014 Jun 23;4:5400. doi: 10.103

    Insert

    humanized S. pyogenes Cas9 (D10A) nickase
    

    Use

    CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    D10A nickase-converting mutation

    Promoter

    CBh

    Available Since

    Nov. 7, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge