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Plasmid#186402PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter eCFP under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only
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pDEST-pSP172BSSPE-pFOGc::RfA-venus
Plasmid#186401PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter Venus under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-pFOGc::venus-RfA
Plasmid#186400PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::venus-RfA
Plasmid#186398PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of N-ter Venus and a gene of interest under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceSept. 29, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L3-a-element-L4
Plasmid#186366PurposeEntry clone with ORF encoding Otx cis-regulatory region flanked by Gateway recombination sequencesDepositorInserta-element early Otx neural enhancer
UseProtein expressionTagsExpressionMutationPromoterT7 promoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pENT-L1-Kozak-lacZ-stop-L2
Plasmid#186359PurposeEntry clone with ORF encoding lacZ flanked by Gateway recombination sequences.DepositorInsertlacZ (lacZ Synthetic, E. coli)
UseExpression of lacz reporterTagsExpressionMutationPromoterAvailable sinceSept. 28, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDEST-pSP172BSSPE-Swa1::RfA-HA
Plasmid#186405PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of a reg. sequence in the Swa1 restriction site.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
PDEST-pSP172BSSPE-pFOGc::RfA-HA
Plasmid#186406PurposeDestination vector for ascidian transgenesis by electroporation of a Gateway-cloned fusion of a gene of interest with a C-ter HA tag under control of the pFOG ectodermal regulatory sequence.DepositorTypeEmpty backboneUseGateway destination vectorTagsExpressionMutationPromoterAvailable sinceAug. 9, 2022AvailabilityAcademic Institutions and Nonprofits only -
pDONR221 CARD8 FIIND
Plasmid#169963PurposepDONR221 for Gateway shuttling. Contains the FIIND (L162M-P434, no stop codon)DepositorInsertCARD8 (CARD8 Human)
UseGateway cloning vectorTagsExpressionMutationPromoterAvailable sinceMay 12, 2021AvailabilityAcademic Institutions and Nonprofits only -
pTEI037
Plasmid#105413PurposeSynthetic biologyDepositorInsertHormone-binding domain (glucocorticoid receptor) fused to mEGFP
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pTEI041
Plasmid#105414PurposeSynthetic biologyDepositorInsertHormone-binding domain and regulatory sequences (estrogen receptor)
UseSynthetic BiologyTagsExpressionMutationBsaI/ BpiI restriction sites removedPromoterAvailable sinceAug. 1, 2018AvailabilityAcademic Institutions and Nonprofits only -
pSBbi G418R mbIL21
Plasmid#194329PurposeConsitiutive mammalian expression of membrane bound human IL-21DepositorInsertmbIL-21 (IL21 Human)
UseTagsExpressionMammalianMutationPromoterEF1-aAvailable sinceJan. 30, 2023AvailabilityAcademic Institutions and Nonprofits only -
pSBbi 41BBL-CD86 mIL-15
Plasmid#183245PurposeExpression of human 41BBL, CD86, and membrane bound human IL-15 (IL-15-IL-15Ra) in a Sleeping Beauty transposon vector for use in artificial antigen presenting cellsDepositorInsert41BBL, CD86, mbIL-15 (IL-15-IL-15Ra)
UseTagsExpressionMammalianMutationPromoterAvailable sinceAug. 18, 2022AvailabilityAcademic Institutions and Nonprofits only -
ZJOM43
Plasmid#133652PurposeIntegration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-VPS4-DuDre (VPS4 Budding Yeast)
UseTagsDuDreExpressionYeastMutationPromoterAvailable sinceAug. 10, 2020AvailabilityAcademic Institutions and Nonprofits only -
pLEX305-N-dTAG-CARD8
Plasmid#169989PurposeMammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)DepositorInsertCARD8 (CARD8 Human)
UseTagsdTAGExpressionMammalianMutationPromoterhPGKAvailable sinceMay 17, 2021AvailabilityAcademic Institutions and Nonprofits only -
EGFP-BAF_L58R
Plasmid#101776PurposeExpresses EGFP tagged mutant BAF (L58R) in human cellsDepositorInsertBAF (BANF1) (BANF1 Human)
UseLentiviralTagsEGFPExpressionMammalianMutationL58R mutationPromoterEF1aAvailable sinceJan. 8, 2018AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 LIC 6A CARD8-FLAG
Plasmid#169948PurposeMammalian expression of CARD8-FLAGDepositorInsertCARD8 (CARD8 Human)
UseTagsFLAGExpressionMammalianMutationPromoterCMVAvailable sinceJune 22, 2021AvailabilityAcademic Institutions and Nonprofits only -
pcDNA3.1 CARD8-TEV-GFP-FLAG
Plasmid#169991PurposeMammalian purification construct for CARD8DepositorInsertCARD8 (CARD8 Human)
UseTagsTEV-GFP-FLAGExpressionMammalianMutationPromoterCMVAvailable sinceJuly 1, 2021AvailabilityAcademic Institutions and Nonprofits only -
ZJOM149
Plasmid#133646PurposeIntegration of ELO3-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).DepositorInsertp1k-ELO3-mCherry (ELO3 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only -
ZJOM44
Plasmid#133653PurposeIntegration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.DepositorInsertp1k-SNF7-mCherry (SNF7 Budding Yeast)
UseTagsmCherryExpressionYeastMutationPromoterAvailable sinceFeb. 12, 2020AvailabilityAcademic Institutions and Nonprofits only