Addgene

We narrowed to 26,211 results for: sta

Showing: 1041 - 1060 of 26211 results

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Emerin pEGFP-C1 (637)

Plasmid

#61993

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Zuleger et al J Cell Biol. 2011 Apr 4;193(1):109-23. d

Insert

Emerin (EMD Human)

Use

Tags

EGFP

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 28, 2015

Availability

Academic Institutions and Nonprofits only

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pSADB19-dTomato Barcode Library

Pooled Library

#227671

Purpose

RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of SADB19 strain G-deleted rabies virus.

Depositor

Cathryn Cadwell, Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Expression

Mammalian

Species

Synthetic

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only
Mouse Chromatin Regulator Library

Pooled Library

#200011

Purpose

CRISPR KO library targeting a comprehensive set of genes with annotated function as chromatin regulators.

Depositor

Robert Manguso, Bradley Bernstein, Gabriel Griffin

Article

Griffin et al Nature. 2021 Jul;595(7866):309-314. doi:

Expression

Mammalian

Use

CRISPR and Lentiviral

Available since

Dec. 15, 2023

Availability

Academic Institutions and Nonprofits only
pCVSN2c-MCP-oScarlet-KASH-6xMS2 Barcode Library

Pooled Library

#227672

Purpose

RVdG genome plasmids that carry an error-correctable barcode compatible with 3’ capture single cell or single nuclei RNA sequencing readout. For the production of CVS N2c strain G-deleted rabies virus

Depositor

Cathryn Cadwell, Tomasz Nowakowski

Article

Shin et al bioRxiv [Preprint]. 2024 Dec 11:2024.10.

Expression

Mammalian

Species

Synthetic

Available since

Feb. 12, 2025

Availability

Academic Institutions and Nonprofits only
pAM-DCA-HA-Ast-3-NP-1-IRES-mCherry-WPRE

Plasmid

#159630

Purpose

AAV expression of HA Allatostatin-3, neurophysin, IRES mCherry under the CAG promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

AAV

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

DCA (same as CAG promoter)

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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pCSC-CMV-HA-Ast-3-NP-1-IRES-mCherry

Plasmid

#159631

Purpose

expresses HA Allatostatin-3, Neurophysin-1, IRES mCherry under the CSC promoter

Depositor

Ross Bathgate, Andrew Allen

Article

Ngo et al Cell Rep. 2020 Sep 15;32(11):108139. doi

Insert

Ast (AstA Fly)

Use

Lentiviral

Tags

HA, IRES, mCherry, and neurophysin-1

Expression

Mammalian

Mutation

Promoter

CMV promoter

Available since

Oct. 30, 2020

Availability

Academic Institutions and Nonprofits only

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Toronto KnockOut version 3 library (TKOv3)

Pooled Library

#90294

Purpose

Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

Depositor

Jason Moffat

Article

Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

Available since

May 5, 2017

Availability

Academic Institutions and Nonprofits only
pEFBOS_CreIRESpuro

Plasmid

#183812

Purpose

Expresses Cre recombinase and puromycin N-acetyltransferase driven by the mammalian EF1a promoter

Depositor

Ed Stanley

Article

Davis et al Nat Protoc. 2008;3(10):1550-8. doi: 10.1

Insert

Cre recombinase

Use

Synthetic Biology

Tags

NLS

Expression

Mammalian

Mutation

Promoter

Available since

Oct. 26, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-IRF9

Plasmid

#71452

Purpose

Lentiviral expression of IRF9

Depositor

George Stark

Article

Cheon et al EMBO J. 2013 Oct 16;32(20):2751-63. doi:

Insert

IRF9 (IRF9 Human)

Use

Lentiviral

Tags

FLAG

Expression

Mutation

Promoter

CMV

Available since

Nov. 30, 2015

Availability

Academic Institutions and Nonprofits only

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pHR-SFFV-PYL1-sfGFP-Emerin

Plasmid

#121532

Purpose

expression of PYL-SfGFP-Emerin, lentivirus vector

Depositor

Stanley Qi

Article

Wang et al Cell. 2018 Nov 15;175(5):1405-1417.e14.

Insert

Emerin (EMD Human)

Use

Lentiviral

Tags

PYL1-SFGFP

Expression

Mammalian

Mutation

Promoter

SFFV

Available since

Feb. 14, 2019

Availability

Academic Institutions and Nonprofits only

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pLenti GW V5 Eco/Dam mse EMD

Plasmid

#182675

Purpose

Lentiviral eukaryotic expression vector with E.Coli Dam methylation fused to mouse Emerin. Used in DamID experiments to methylate DNA that interacts in the proximity of Emerin.

Depositor

Bas van Steensel

Article

Amendola et al EMBO Rep. 2015 May;16(5):610-7. doi: 10.

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

E. Coli Dam and V5

Expression

Mammalian

Mutation

Promoter

HSP

Available since

April 22, 2022

Availability

Academic Institutions and Nonprofits only

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pLV-EF1a-V5-Emerin

Plasmid

#120246

Purpose

Expresses V5-tagged Emerin in lentiviral vector

Depositor

Larry Gerace

Article

Cheng et al Nucleus. 2019 Dec;10(1):126-143. doi: 10

Insert

Emerin (Emd Mouse)

Use

Lentiviral

Tags

V5

Expression

Mutation

Promoter

EF1a

Available since

Jan. 9, 2019

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-UTPase

Plasmid

#12667

Depositor

SeWon Suh

Article

Han et al Acta Crystallogr D Biol Crystallogr. 200

Insert

dUTP pyrophosphatase (DUT1 Budding Yeast)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-RNase HIII

Plasmid

#12683

Depositor

SeWon Suh

Article

Kwak et al Acta Crystallogr D Biol Crystallogr. 200

Insert

RNase HIII

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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pET16B.Pfu

Plasmid

#12509

Purpose

Bacterial expression of Pfu DNA polymerase

Depositor

Sung-Hou Kim

Article

Goldman et al Acta Crystallogr D Biol Crystallogr. 199

Insert

Pfu DNA polymerase

Use

Tags

Expression

Bacterial

Mutation

Y218H, Q462R, F533L, K559I and V683A (mutations w…

Promoter

Available since

Sept. 18, 2006

Availability

Academic Institutions and Nonprofits only

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pET-22b(+)-HpENR

Plasmid

#12651

Depositor

SeWon Suh

Article

Lee et al Acta Crystallogr D Biol Crystallogr. 200

Insert

noyl-acyl carrier protein reductase (fabI Helicobacter pylori)

Use

Tags

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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TrmD_HaeIn/pET28b(+) N,C-tag

Plasmid

#12665

Depositor

SeWon Suh

Article

Kim et al Acta Crystallogr D Biol Crystallogr. 200

Insert

tRNA (guanine-N(1)-)-methyltransferase (trmD Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

Jan. 5, 2007

Availability

Academic Institutions and Nonprofits only

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HI0058/pET-21a(+)c-tag

Plasmid

#13097

Depositor

SeWon Suh

Article

Ku et al Acta Crystallogr D Biol Crystallogr. 200

Insert

3-deoxy-manno-octulosonate cytidylyltransferase (kdsB Haemophilus influenzae)

Use

Tags

His

Expression

Bacterial

Mutation

Promoter

Available since

June 29, 2007

Availability

Academic Institutions and Nonprofits only

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pISV-DsRed1

Plasmid

#171024

Purpose

Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus.

Depositor

Christian Staehelin

Type

Empty backbone

Use

Tags

Expression

Plant

Mutation

Promoter

2X EN 35S

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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pISV-DsRed1-nopP

Plasmid

#171025

Purpose

Agrobacterium rhizogenes-mediated hairy root transformation of Lotus japonicus with nopP, a type 3 effector gene of Sinorhizobium sp. NGR234.

Depositor

Christian Staehelin

Insert

nopP

Use

Tags

Expression

Plant

Mutation

Promoter

2 X EN 35S

Available since

Aug. 5, 2021

Availability

Academic Institutions and Nonprofits only

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