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pGL3con-miR15aBul

Plasmid

#21117

Depositor

Joshua Mendell

Article

Hwang et al Proc Natl Acad Sci U S A. 2009 Apr 28;10

Insert

hsa-miR-15a sensor

Expression

Mammalian

Available Since

May 13, 2009

Availability

Academic Institutions and Nonprofits only

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AIRAP 4: AIRAP.GST.V3.pCDNA3

Plasmid

#21794

Depositor

David Ron

Article

Stanhill et al Mol Cell. 2006 Sep 15. 23(6):875-85.

Insert

AIRAP (Zfand2a Mouse)

Tags

GST

Expression

Mammalian

Available Since

Sept. 10, 2009

Availability

Academic Institutions and Nonprofits only

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pBSKΔB-CBh-Cas9n-pA

Plasmid

#82617

Purpose

Expresses Cas9 nickase in mammalian cells

Depositor

Hiroshi Ochiai

Article

Seirin-Lee et al PLoS Comput Biol. 2019 Sep 11;15(9):e100

Insert

SpCas9n

Use

CRISPR

Expression

Mammalian

Available Since

Sept. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP DNAJB3

Plasmid

#19498

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

DNAJB3

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pcDNA5/FRT/TO GFP SSA1

Plasmid

#19489

Depositor

Harm Kampinga

Article

Hageman et al Cell Stress Chaperones. 2009 Jan;14(1):1

Insert

SSA1 (SSA1 Budding Yeast)

Tags

GFP

Expression

Mammalian

Available Since

Oct. 7, 2008

Availability

Academic Institutions and Nonprofits only

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pISlin41m(s) (lin-41 microRNA mut reporter)

Plasmid

#11907

Depositor

David Bartel

Article

Lewis et al Cell. 2003 Dec 26. 115(7):787-98.

Insert

lin-41 miRNA target sequence mut (lin-41 Nematode)

Use

Luciferase

Expression

Mammalian

Mutation

point mutations in miRNA complementary site

Available Since

April 27, 2007

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 human APC-B,C (1133–1189)

Plasmid

#132630

Purpose

expresses human APC-B,C (1133–1189) in E. coli

Depositor

Wenqing Xu

Article

Xing et al Genes Dev. 2003 Nov 15;17(22):2753-64. d

Insert

APC-B,C (1133–1189)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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pOX-twk43

Plasmid

#16128

Depositor

Larry Salkoff

Article

Salkoff et al Neuroscience. 2001 . 103(4):853-9.

Insert

Tandem pore domain K+ channel (F32H5.2, twk-43 Nematode)

Use

Xenopus oocyte expression

Mutation

N/A

Available Since

Jan. 18, 2008

Availability

Academic Institutions and Nonprofits only
pFA6a-kanMX6-P81nmt1-mCFP

Plasmid

#105142

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P81nmt1-mCFP

Tags

81nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

81nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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pFA6a-kanMX6-P41nmt1-mCFP

Plasmid

#105139

Purpose

Yeast gene targeting

Depositor

Thomas Pollard, Jian-Qiu Wu

Article

Wu et al Dev Cell. 2003 Nov;5(5):723-34.

Insert

kanMX6-P41nmt1-mCFP

Tags

41nmt1-mCFP

Expression

Bacterial and Yeast

Mutation

A206K

Promoter

41nmt1

Available Since

March 19, 2018

Availability

Academic Institutions and Nonprofits only

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mCerulean3-MCHR1-N-10

Plasmid

#55432

Purpose

Localization: Cilium, Excitation: 433, Emission: 475

Depositor

Michael Davidson

Article

Markwardt et al PLoS One. 2011 . 6(3):e17896.

Insert

MCHR1

Tags

mCerulean3

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 7, 2014

Availability

Academic Institutions and Nonprofits only
Degron-KI PIK3CA donor vector

Plasmid

#65621

Purpose

Donor vector to generate Degron-KI DD-PIK3CA

Depositor

Frank Stegmeier

Article

Zhou et al Cancer Res. 2015 Mar 18. pii: canres.293

Insert

PIK3CA Degron-KI left and right HR arms

Available Since

June 8, 2015

Availability

Academic Institutions and Nonprofits only
pMK33/intein-Cas9_S219-3XFLAG

Plasmid

#133782

Purpose

Expresses human codon-optimized intein-Cas9

Depositor

Norbert Perrimon

Article

Viswanatha et al Elife. 2018 Jul 27;7. pii: 36333. doi: 1

Insert

Human codon-optimized intein-Cas9

Expression

Insect

Available Since

Jan. 31, 2020

Availability

Academic Institutions and Nonprofits only

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pSL6 SMR1747

Plasmid

#28029

Depositor

Susan Rosenberg

Article

Harris et al Genes Dev. 1997 Sep 15. 11(18):2426-37.

Insert

MutS (mutS E. coli)

Expression

Bacterial

Available Since

April 1, 2011

Availability

Academic Institutions and Nonprofits only
pBRIT Pax7 34C

Plasmid

#17522

Depositor

Michael Rudnicki

Article

McKinnell et al Nat Cell Biol. 2008 Jan . 10(1):77-84.

Insert

Pax7 (Pax7 Mouse)

Use

Cre/Lox and Retroviral

Tags

FLAG

Expression

Mammalian

Mutation

Deletion of aa 1-33

Available Since

March 12, 2008

Availability

Academic Institutions and Nonprofits only
p3994 pGEM HHR23A

Plasmid

#10863

Depositor

Peter Howley

Article

Kumar et al J Biol Chem 1997 May 23;272(21):13548-54

Insert

HHR23A (RAD23A Human)

Expression

Bacterial

Available Since

Oct. 26, 2005

Availability

Academic Institutions and Nonprofits only
pCF226

Plasmid

#121667

Purpose

EFS-Cas9-wt-P2A-Puro

Depositor

David Savage

Article

Oakes et al Cell. 2019 Jan 10;176(1-2):254-267.e16.

Insert

cas9

Use

Lentiviral

Promoter

EF-1a core promoter

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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Hy_pMtnA nLuc SV40

Plasmid

#132654

Purpose

Expresses nanoLuciferase (nLuc) mRNA from the Drosophila Metallothionein A promoter

Depositor

Jeremy Wilusz

Article

Tatomer et al Genes Dev. 2019 Sep 17. pii: gad.330167.

Insert

nanoLuciferase

Tags

3xFLAG

Expression

Insect

Promoter

MtnA

Available Since

Oct. 3, 2019

Availability

Academic Institutions and Nonprofits only

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pMSCV-PIG-miR-26a-2

Plasmid

#64230

Purpose

Expresses miR-26a-2 in mammalian cells

Depositor

Joshua Mendell

Article

Chang et al Nat Genet. 2008 Jan;40(1):43-50. Epub 20

Insert

miR-26a-2 (MIR26A2 Human)

Use

Retroviral

Expression

Mammalian

Available Since

Nov. 13, 2015

Availability

Academic Institutions and Nonprofits only

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pGGD007

Plasmid

#48837

Purpose

Provides linker:nuclear localization signal as GreenGate C-terminal tag module.

Depositor

Jan Lohmann

Article

Lampropoulos et al PLoS One. 2013 Dec 20;8(12):e83043. doi:

Insert

linker:nuclear localization signal

Use

Golden gate compatible cloning vector

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only

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