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We narrowed to 24,091 results for: CRISPR

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Showing: 10721 - 10740 of 24091 results

  4. qTAG-C-Solo-mStayGold-TOMM20

    Plasmid
    #227307
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the TOMM20 locus. For mitochondria visualization. To be co-transfected with sgRNA plasmid px330-PITCh-TOMM20 (Addgene #207789)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    TOMM20 Homology Arms flanking a mStayGold Tag (TOMM20 Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227307-plasmid-map-sequence-id-451546

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  9. pSL1145 (pSPIN, pBBR1 backbone, R*MmeI)

    Plasmid
    #160736
    Purpose
    Single-plasmid V. cholerae CAST, encodes all proteins, crRNA, and donor DNA. Non-targeting crRNA with BsaI sites for spacer cloning. Mini-tn has MmeI site in R end for Tn-seq. pBBR1 backbone.
    Depositor
    Samuel H. Sternberg
    Article
    Vo et al Nat Biotechnol. 2020 Nov 23. pii: 10.103
    Insert
    VchCAST proteins, crRNA, and donor DNA
    Expression
    Bacterial
    Promoter
    J23119
    Available Since
    March 10, 2021
    Availability
    Academic Institutions and Nonprofits only
    160736-plasmid-map-sequence-id-326992

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  10. qTAG-C-Solo-mStayGold-COX8A

    Plasmid
    #227309
    Purpose
    Donor template for mStayGold insertion into the C-terminus of the COX8A locus. For mitochondria visualization. To be co-transfected with sgRNAplasmid px330-PITCh-COX8A (Addgene #227308)
    Depositor
    Laurence Pelletier
    Article
    Philip et al EMBO J. 2024 Dec 12. doi: 10.1038/s44318
    Insert
    COX8A Homology Arms flanking a mStayGold Tag (COX8A Human)
    Use
    CRISPR; Donor template
    Expression
    Mammalian
    Promoter
    Promoterless
    Available Since
    Nov. 25, 2024
    Availability
    Academic Institutions and Nonprofits only
    227309-plasmid-map-sequence-id-451688

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  12. pJEP10-AAV-U6/TO-gRNA(Empty)-CMV-TetR-P2A-eGFP-KASH-pA

    Plasmid
    #82706
    Purpose
    AAV backbone with a full length U6/TO promoter driving expression of a sgRNA. The gRNA can be inserted via Sapl sites. TracrRNA compatible with SpCas9. gRNA transcription is doxycycline dependent.
    Depositor
    Jonathan Ploski
    Article
    de Solis et al Front Mol Neurosci. 2016 Aug 18;9:70. do
    Insert
    U6/TO Empty gRNA Expression Cassette
    Use
    AAV and CRISPR
    Tags
    CMV promoter driving expression of TetR-P2A-GFP-K…
    Expression
    Mammalian
    Available Since
    Dec. 20, 2016
    Availability
    Academic Institutions and Nonprofits only
    82706-plasmid-map-sequence-id-158046

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  16. pcU6_3 MS2 sgRNA

    Plasmid
    #92394
    Purpose
    Chick-specific U6 sgRNA expression mini-vector, harbouring chick U6_3 pol III promoter, with tracrRNA scaffold containing stem loops allowing binding of bacteriophage MS2 coat protein MCP
    Depositor
    Tatjana Sauka-Spengler
    Article
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    chick U6.3 promoter and gRNA cloning cassette including MS2 stem loops
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    chick U6.3
    Available Since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    92394-plasmid-map-sequence-id-191866

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  17. pLCKO

    Plasmid
    #73311
    Purpose
    Lentiviral backbone for cloning and expressing U6 driven sgRNAs with BfuAI cloning sites and puromycin selection.
    Depositor
    Jason Moffat
    Article
    Hart et al Cell. 2015 Dec 3;163(6):1515-26. doi: 10
    Type
    Empty backbone
    Use
    CRISPR and Lentiviral
    Expression
    Mammalian
    Promoter
    U6
    Available Since
    Feb. 4, 2016
    Availability
    Academic Institutions and Nonprofits only
    73311-plasmid-map-sequence-id-134941

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  19. pJZC78

    Plasmid
    #62339
    Purpose
    sgRNA + 1x COM with COM-KRAB effector for mammalian cells
    Depositor
    Wendell Lim, Stanley Qi
    Article
    Zalatan et al Cell. 2014 Dec 18. pii: S0092-8674(14)01
    Inserts
    sgRNA + 1x COM binding module
    COM-KRAB
    Use
    Lentiviral
    Tags
    KRAB
    Expression
    Mammalian
    Mutation
    Targets sv40 promoter, sequence: CATACTTCTGCCTGCT…
    Promoter
    CMV and U6
    Available Since
    April 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    62339-plasmid-map-sequence-id-102262

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Showing: 10721 - 10740 of 24091 results