We narrowed to 17,871 results for: SHE

1. CDK4-luciferase full length (2135)

   Plasmid

   #86656

   Purpose

   Luciferase reporter containing CDK4 promoter

   Depositor

   Jaime Modiano

   Article

   Baksh et al Mol Cell. 2002 Nov;10(5):1071-81.

   Insert

   CDK4 promoter (CDK4 Human)
   

   Use

   Luciferase

   Tags

   luciferase

   Expression

   Mammalian

   Mutation

   2120 base pairs including up to coding nucleotide…

   Promoter

   CDK4

   Available since

   June 20, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pKT-mIDH1(R132H)-Myc-FLAG-IRES-Kat

   Plasmid

   #133779

   Purpose

   Expresses IDH1 with a R132H mutation and has Myc and FLAG tags. IDH1(R132H) also has a katushka fluorescent reporter. Construct has inverted repeats to be used in Sleeping Beauty transposon system.

   Depositor

   Maria Castro

   Insert

   Isocitrate dehydrogenase 1 (IDH1 Human)
   

   Use

   Tags

   Myc and FLAG

   Expression

   Mammalian

   Mutation

   Arginine is mutated to histidine at amino acid po…

   Promoter

   Available since

   Oct. 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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3. pUASp YFP Ras85D WT

   Plasmid

   #52242

   Purpose

   Drosophila expression of YFP-Ras85D

   Depositor

   Matthew Scott

   Insert

   Ras85D (Ras85D Fly)
   

   Use

   Drosophila

   Tags

   YFP

   Expression

   Insect

   Mutation

   Promoter

   GAL4

   Available since

   March 20, 2014

   Availability

   Academic Institutions and Nonprofits only

4. pPB-MPP8_Flag2-Blast

   Plasmid

   #175038

   Purpose

   Mammalian expression of full length mMPP8 with C-terminal Flag2 tag

   Depositor

   Kristian Helin

   Article

   Muller et al Nat Commun. 2021 May 24;12(1):3034. doi:

   Insert

   Mphosph8 (Mphosph8 Mouse)
   

   Use

   Piggybac

   Tags

   Flag2

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Sept. 24, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pTrc99S-ssDsbA-TTc-4xDQNAT

   Plasmid

   #128401

   Purpose

   Periplasmic expression of tetanus toxin fragment C with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin fragment C domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. pTrc99S-ssDsbA-TTlight-4xDQNAT

   Plasmid

   #128402

   Purpose

   Periplasmic expression of tetanus toxin light chain with C terminal 4xDQNAT glycosylation sites in E. coli

   Depositor

   Michael Jewett

   Article

   Stark et al Sci Adv. 2021 Feb 3;7(6). pii: 7/6/eabe9

   Insert

   Tetanus toxin light chain domain
   

   Use

   Tags

   4xDQNAT glycosylation tag, 6xHis tag, and DsbA si…

   Expression

   Bacterial

   Mutation

   Inactivating E234A mutation

   Promoter

   trc

   Available since

   April 13, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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7. pSR357.10

   Plasmid

   #125106

   Purpose

   Expression of narL(1-131aa)-ydfI(126-213aa) chimera under PLtetO-1, output Promoter PydfJ115

   Depositor

   Jeffrey Tabor

   Article

   Schmidl et al Nat Chem Biol. 2019 Jul;15(7):690-698. d

   Inserts

   narL(REC)-ydfI(DBD)131
   sfgfp
   tetR
   

   Use

   Synthetic Biology

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   June 26, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. (326) pAAV SV40-ZsGreen U6-gRNA

   Plasmid

   #163020

   Purpose

   Fluorescent reporter for Sa gRNA (Bsa1 sites)

   Depositor

   Tonia Rex

   Article

   Backstrom et al Mol Ther Methods Clin Dev. 2020 Sep 6;19

   Insert

   Zs Green
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   SV40

   Available since

   Jan. 8, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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9. pRC11-U6-DR-crRNA-BsmbI(x2); EFS-Puro-WPRE

   Plasmid

   #123360

   Purpose

   Lentiviral vector with empty U6 cassette containing LbCpf1 direct repeat, and constitutive expression of puromycin resistance.

   Depositor

   Sidi Chen

   Article

   Chow et al Nat Methods. 2019 May;16(5):405-408. doi

   Insert

   Empty LbCpf1 crRNA cloning cassette
   

   Use

   CRISPR and Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   U6

   Available since

   May 14, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. GA-DEVD-GB-NES

    Plasmid

    #61020

    Purpose

    green ddFP based caspase-3 biosensor

    Depositor

    Robert Campbell

    Article

    Ding et al Nat Methods. 2015 Jan 26. doi: 10.1038/n

    Insert

    ddGFP A and ddGFP B
    

    Use

    Tags

    HindIII and NES sequence LALKLAGLDIGS placed afte…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 28, 2015

    Availability

    Academic Institutions and Nonprofits only

11. plc242-GFP-WDR24

    Plasmid

    #87050

    Purpose

    mammalian expression of WDR24

    Depositor

    David Sabatini

    Article

    Wolfson et al Nature. 2017 Feb 15. doi: 10.1038/nature

    Insert

    WDR24 (WDR24 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 28, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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12. mCardinal-Paxillin-22

    Plasmid

    #56171

    Purpose

    Localization: Focal Adhesions, Excitation: 604, Emission: 659

    Depositor

    Michael Davidson

    Article

    Chu et al Nat Methods. 2014 Mar 16. doi: 10.1038/n

    Insert

    Paxillin
    

    Use

    Tags

    mCardinal

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Feb. 27, 2015

    Availability

    Industry, Academic Institutions, and Nonprofits

13. pBV-luc TOP2A 1kb promoter

    Plasmid

    #138274

    Purpose

    Luciferase reporter plasmid driven by TOP2A promoter

    Depositor

    Wen Xue

    Article

    Kwan et al Hepatology. 2019 Jun 12. doi: 10.1002/he

    Insert

    TOP2A promoter sequence
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    March 18, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. pBabe_puro_DEST_Flag_SKIL

    Plasmid

    #45265

    Depositor

    William Hahn

    Article

    Hagerstrand et al Cancer Discov. 2013 Jun 13. [Epub ahead

    Insert

    SNON (SKIL Human)
    

    Use

    Retroviral

    Tags

    Flag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 22, 2013

    Availability

    Academic Institutions and Nonprofits only

15. pCDNA3-MARS-p85(iSH2)

    Plasmid

    #205236

    Purpose

    Expresses PLEKAH5 aa 143-271 (K163A and R164A) fused to the iSH2 domain of mouse p85beta

    Depositor

    Jeremy Baskin

    Inserts

    PLEKHA5(143-271)-K163A/R164A (PLEKHA5 Human)
    p85(iSH2) (Pik3r1 Mouse)
    

    Use

    Tags

    Nuclear Export Sequence and mCherry

    Expression

    Mammalian

    Mutation

    amino acids 143-271 of PLEKHA5 (GenBank reference…

    Promoter

    CMV

    Available since

    July 30, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRK5 CASTOR2-Flag Cterm

    Plasmid

    #84490

    Purpose

    overexpression of CASTOR2 with Flag tag

    Depositor

    David Sabatini

    Article

    Chantranupong et al Cell. 2016 Mar 24;165(1):153-64. doi: 10

    Insert

    CASTOR2 (GATSL2 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Oct. 24, 2016

    Availability

    Academic Institutions and Nonprofits only

17. pET151-Vps4(101-437)-Hcp1

    Plasmid

    #87737

    Purpose

    Bacterial expression of S. cerevisiae Vps4 AAA ATPase cassette fused to P. aeruginosa Hcp1

    Depositor

    Chris Hill

    Article

    Monroe et al Elife. 2017 Apr 5;6. pii: e24487. doi: 1

    Insert

    Vacuolar protein sorting-associated protein 4 (VPS4 Budding Yeast)
    

    Use

    Tags

    6xHis-V5-TEV and Hcp1

    Expression

    Bacterial

    Mutation

    deleted amino acids 1-100

    Promoter

    T7

    Available since

    May 12, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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18. pVRC8400-SARS-CoV-2-S2P-BA.4/5-AVI

    Plasmid

    #192112

    Purpose

    Expresses SARS-CoV-2 S2P protein from the BA.4 and BA.5 variants with single chain Fc, HRV3C protease cleavage site, and AVI tag

    Depositor

    Peter Kwong

    Insert

    SARS-CoV-2-S2P-BA.4/5 (S )
    

    Use

    Tags

    AVI, HRV3C, and scFc

    Expression

    Mammalian

    Mutation

    T19I, L24S, P25-, P26-, A27-, H69-, V70-, G142D, …

    Promoter

    Available since

    Oct. 20, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pGEMHE-Pom121-mScarlet

    Plasmid

    #171491

    Purpose

    T7 promotor drives in vitro transcription of mScarlet-tagged mouse Pom121 mRNA

    Depositor

    Melina Schuh

    Article

    Cavazza et al Cell. 2021 Apr 30. pii: S0092-8674(21)00

    Insert

    Pom121 (Pom121 Mouse)
    

    Use

    Pgehme

    Tags

    mScarlet

    Expression

    Mutation

    Promoter

    T7

    Available since

    Aug. 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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20. pCMV_Anc689-nCas9

    Plasmid

    #163526

    Purpose

    Mammalian CG-to-GC base editing

    Depositor

    David Liu

    Article

    Koblan et al Nat Biotechnol. 2021 Jun 28. pii: 10.103

    Insert

    Anc689-nCas9
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    nCas9 (D10A)

    Promoter

    Available since

    Dec. 21, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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