We narrowed to 32,291 results for: uros

1. CJ45 (miR-1003 5' splice site mut)

   Plasmid

   #17775

   Depositor

   David Bartel

   Article

   Ruby et al Nature. 2007 Jul 5. 448(7149):83-6.

   Insert

   dme-miR-1003 minigene 5' splice mut
   

   Use

   Drosophila metallothionein gene promoter drives e…

   Expression

   Insect

   Mutation

   5' splice site mut + compsensatory stem

   Available Since

   April 15, 2008

   Availability

   Academic Institutions and Nonprofits only

2. pOX + JShal XR

   Plasmid

   #16148

   Depositor

   Larry Salkoff

   Article

   Jegla et al J Neurosci. 1997 Jan 1. 17(1):32-44.

   Insert

   Polyorchis penicillatus potassium channel gamma subunit (LOC721209, Ggamma1 jelly fish)
   

   Use

   Xenopus oocyte expression

   Mutation

   N/A

   Available Since

   Jan. 10, 2008

   Availability

   Academic Institutions and Nonprofits only

3. pTorPE-Y-GECO2s

   Plasmid

   #105067

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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4. pTorPE-Y-GECO2.1s

   Plasmid

   #105068

   Purpose

   A yellow fluorescent Ca2+ indicator variant with high binding affinity and slow kinetics for expression in bacterial cells

   Depositor

   Robert Campbell

   Article

   Zhao et al Sci Rep. 2018 Aug 6;8(1):11758. doi: 10.

   Insert

   Y-GECO2.1s
   

   Tags

   6x His tag

   Expression

   Bacterial

   Promoter

   pBAD

   Availability

   Academic Institutions and Nonprofits only

   

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5. pAAV-CAG-EGFP barcode-5-SV40 polyA

   Plasmid

   #190869

   Purpose

   For barcoded retrograde labeling

   Depositor

   Yuejun Chen

   Article

   Xu et al Elife. 2024 Feb 23;13:e85419. doi: 10.75

   Insert

   EGFP-barcode5
   

   Use

   AAV

   Mutation

   WT

   Available Since

   Nov. 14, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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6. mito NAD+ biosensor

   Plasmid

   #186791

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag totarget mitochondria

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   mito NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pCAG-HIF1 alpha

   Plasmid

   #21101

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1ALPHA (HIF1A Human)
   

   Expression

   Mammalian

   Available Since

   May 5, 2009

   Availability

   Academic Institutions and Nonprofits only

   

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8. cytoplasmic NAD+ biosensor

   Plasmid

   #186787

   Purpose

   NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the cytoplasm

   Depositor

   Michael Cohen

   Article

   Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

   Insert

   cytoplasmic NAD+ biosensor
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   July 28, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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9. pCAG-HIF1 alpha K532R

   Plasmid

   #21102

   Depositor

   Connie Cepko

   Article

   Chen et al Science. 2009 Jan 9. 323(5911):256-9.

   Insert

   HYPOXIA INDUCIBLE FACTOR 1 ALPHA-K532R MUTANT (HIF1A Human)
   

   Expression

   Mammalian

   Available Since

   Aug. 6, 2009

   Availability

   Academic Institutions and Nonprofits only

10. pAAVS1-P-MCS

    Plasmid

    #80488

    Purpose

    donor vector for AAVS1 targeting (puromycin selection) with multiple cloning site

    Depositor

    Knut Woltjen

    Article

    Oceguera-Yanez et al Methods. 2015 Dec 18. pii: S1046-2023(15

    Type

    Empty backbone

    Use

    Donor vector (human)

    Expression

    Mammalian

    Available Since

    Nov. 28, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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11. nuclear NAD+ biosensor

    Plasmid

    #186789

    Purpose

    NAD+ biosensor comprised of a circularly permuted Venus fluorescent protein (cpVenus), a bipartite NAD+-binding domain modeled from bacterial DNA ligase, and a localization tag to target the nucleus

    Depositor

    Michael Cohen

    Article

    Cambronne et al Science. 2016 Jun 17;352(6292):1474-7. d

    Insert

    nuclear NAD+ biosensor
    

    Use

    Lentiviral

    Expression

    Mammalian

    Available Since

    July 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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12. pLX304_CMV::mCherry-LgBiT-FLAG-NES

    Plasmid

    #199715

    Purpose

    Expresses LgBiT, fused to mCherry, in the cytosol of mammalian cells. Can be used in generating stable cell lines via lentiviral transduction.

    Depositor

    Alice Ting

    Article

    Roh et al J Am Chem Soc. 2023 May 10;145(18):10220

    Insert

    mCherry-LgBiT-FLAG-NES
    

    Use

    Lentiviral and Luciferase

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    May 9, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. PB-CT3G-CERP2-MG

    Plasmid

    #175503

    Purpose

    All-in-one piggyBac transposon destination vector for mCMV+dox-inducible expression of Mega Gate cloned elements (CMVe-hEF1a-driven rtTA and puromycin resistance)

    Depositor

    George Church

    Article

    Kramme et al Cell Rep Methods. 2021 Sep 27;1(6):10008

    Type

    Empty backbone

    Use

    Synthetic Biology

    Expression

    Mammalian

    Promoter

    TRE3G-mCMV

    Available Since

    Oct. 8, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-EF1a-DIO-ChrimsonR-mRuby2-KV2.1-WPRE-SV40

    Plasmid

    #124603

    Purpose

    Cre-dependent soma-localized ChrimsonR for optogenetic stimulation

    Depositor

    Christopher Harvey

    Article

    Chettih et al Nature. 2019 Mar 6. pii: 10.1038/s41586-

    Has Service

    AAV9

    Insert

    Soma-localized ChrimsonR
    

    Use

    AAV

    Tags

    mRuby2

    Expression

    Mammalian

    Available Since

    April 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. Flag-HA-ATXN3

    Plasmid

    #22538

    Depositor

    Wade Harper

    Article

    Sowa et al Cell. 2009 Jul 23. 138(2):389-403.

    Insert

    ATXN3 (ATXN3 Human)
    

    Use

    Retroviral

    Tags

    FLAG and HA

    Expression

    Mammalian

    Mutation

    None

    Available Since

    Nov. 13, 2009

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRSETB/mStayGold

    Plasmid

    #212017

    Purpose

    mStayGold has an n1 adaptor at its N-terminus and is useful for C-terminal tagging. This construct is used also for expression of mStayGold itself.

    Depositor

    Atsushi Miyawaki

    Article

    Ando et al Nat Methods. 2023 Nov 30. doi: 10.1038/s

    Insert

    mStayGold
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Jan. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pOlyA(E69A)

    Plasmid

    #132392

    Purpose

    Mutant version of OlyA that senses sphingomyelin without or with cholesterol

    Depositor

    Arun Radhakrishnan

    Article

    Endapally et al Cell. 2019 Feb 21;176(5):1040-1053.e17.

    Insert

    Ostreolysin A point mutant (E69A)
    

    Tags

    His6 tag

    Expression

    Bacterial

    Mutation

    Mutations: C62S, E69A, C94S, S151C

    Available Since

    Sept. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. pCAG mito-GCaMP5G

    Plasmid

    #105009

    Purpose

    mitochondrial calcium sensor

    Depositor

    Franck Polleux

    Article

    Kwon et al PLoS Biol. 2016 Jul 18;14(7):e1002516. d

    Insert

    GCaMP5G
    

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    Jan. 17, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLenti UBC hNLRP3-mNG

    Plasmid

    #218642

    Purpose

    Expresses NLRP3 tagged with mNeonGreen in mammalian cells (lentiviral); driven by the weak UBC promoter with puro selection

    Depositor

    Wade Harper

    Article

    Hollingsworth et al bioRxiv 2024.04.19.590338

    Insert

    NLRP3 (NLRP3 Human)
    

    Use

    Lentiviral

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    UBC

    Available Since

    May 1, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. p-AAV-sh[control]

    Plasmid

    #75438

    Purpose

    control shRNA vector

    Depositor

    Edward Burton

    Article

    Zharikov et al J Clin Invest. 2015 Jul 1;125(7):2721-35

    Insert

    GFP
    

    Use

    AAV and RNAi

    Expression

    Mammalian

    Promoter

    U6

    Available Since

    Aug. 9, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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