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pCDNA3-HA/Bmal I317D

Plasmid

#47347

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

I317D

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pcDNA 3.1 (+) Tac-delta scr

Plasmid

#80019

Purpose

Mammalian expression of Tac-delta scr

Depositor

Chinten James Lim

Article

Liu et al Mol Cell Biol. 2013 Nov;33(21):4334-45.

Insert

Tac (IL2RA Human)

Tags

KLRFGFK

Expression

Mammalian

Mutation

CD25 amino acids 1 to 263 fused to KLRFGFK

Promoter

CMV

Available Since

July 12, 2016

Availability

Academic Institutions and Nonprofits only
pcDNA4-Tet-on-vsv-Mis12-dCEN Incenp

Plasmid

#108482

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA4-Tet-on-vsv-Mis12-dCEN-Incenp-GFP

Plasmid

#108485

Purpose

inducible expression of vsv-Mis12-dCEN-INCENP-EGFP

Depositor

Susanne Lens

Article

Liu et al Science. 2009 Mar 6;323(5919):1350-3. do

Insert

Mis12-dCEN-INCENP (INCENP Human)

Tags

EGFP and VSV

Expression

Mammalian

Mutation

CEN box replaced by Mis12

Promoter

CMV

Available Since

May 8, 2018

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L115E

Plasmid

#47345

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L115E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pAM4731

Plasmid

#85127

Purpose

NSI-NT expressing KaiB from native promoter. Tested by Topcount: Displays WT rhythms.

Depositor

Susan Golden

Article

Chang et al Science. 2015 Jul 17;349(6245):324-8. do

Insert

PkaiBC-KaiB

Expression

Bacterial

Available Since

July 7, 2017

Availability

Academic Institutions and Nonprofits only

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pCDNA3-HA/Bmal L95E/L150E/W427A

Plasmid

#47352

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

L95E/L150E/W427A

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pHR-LAT-Y6F YYYY-mCitrine (XSB586)

Plasmid

#78515

Purpose

Lentiviral vector for expressing LAT-Y6F YYYY-mCitrine (3 Grb2 binding sites)

Depositor

Ron Vale

Article

Su et al Science. 2016 Apr 29;352(6285):595-9. do

Insert

LAT (LAT Human)

Use

Lentiviral

Tags

mCitrine

Expression

Mammalian

Available Since

Aug. 9, 2018

Availability

Academic Institutions and Nonprofits only

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pRK5 HA-GST-Presc-mAkt1 (S473T)

Plasmid

#48806

Purpose

expresses mutant Akt1

Depositor

David Sabatini

Article

Kang et al Science. 2013 Jul 26;341(6144):1236566.

Insert

Akt1 (Akt1 Mouse)

Tags

HA-GST-Presc

Expression

Mammalian

Mutation

S473T

Promoter

CMV

Available Since

May 2, 2014

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 L272A

Plasmid

#19850

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 L272A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Leucine 272 to Alanine

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCHKU34.1-2.2

Plasmid

#115534

Purpose

Expression of a fungal biosynthetic gene cluster

Depositor

Ronald Davis

Article

Harvey et al Sci Adv. 2018 Apr 11;4(4):eaar5459. doi:

Insert

PKS

Expression

Yeast

Available Since

March 5, 2019

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN

Plasmid

#47353

Purpose

Clock fragment cloned and tagged with VenN

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal W427A

Plasmid

#47349

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

W427A

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
PHKA1 gRNA (BRDN0001145492)

Plasmid

#77840

Purpose

3rd generation lentiviral gRNA plasmid targeting human PHKA1

Depositor

John Doench, David Root

Article

Doench et al Nat Biotechnol. 2016 Jan 18. doi: 10.103

Insert

PHKA1 (PHKA1 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

hU6

Available Since

July 6, 2016

Availability

Academic Institutions and Nonprofits only

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pCin4-3xFLAG-MKL1 D444-630

Plasmid

#19840

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 D444-630 (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

deleted amino acids 444-630

Available Since

March 16, 2009

Availability

Academic Institutions and Nonprofits only
pCDNA3-HA/Bmal V435R

Plasmid

#47350

Purpose

PcHA-Bmal backbone used for the above mutation. Ref.Genes & Dev 17:1921-1932 2003

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

BMAL (Bmal1 Mouse)

Tags

Ha

Expression

Mammalian

Mutation

V435R

Promoter

CMV

Available Since

Jan. 7, 2014

Availability

Academic Institutions and Nonprofits only
pCin4-3xFLAG-MKL1 S449A

Plasmid

#19842

Depositor

Ron Prywes

Article

Muehlich et al Mol Cell Biol. 2008 Oct;28(20):6302-13.

Insert

MKL1 S449A (MRTFA Human)

Tags

3xFLAG

Expression

Mammalian

Mutation

changed Serine 449 to Alanine

Available Since

Dec. 15, 2008

Availability

Academic Institutions and Nonprofits only
pUC19-D4J3S7

Plasmid

#103096

Purpose

Cas9 coding gene template with optimized sequence for human codon usage

Depositor

Duhee Bang

Article

Cho et al Nucleic Acids Res. 2018 May 18;46(9):e55

Insert

D4J3S7(Cas9 coding gene from Coprococcus catus GD/7)

Use

CRISPR; Cloning vector

Mutation

human codon-optimized

Available Since

April 20, 2018

Availability

Academic Institutions and Nonprofits only

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pEJS583-pCSDest2-AcrIIC5Smu-FLAG-NLS

Plasmid

#113437

Purpose

Expresses a FLAG/NLS tagged type II-C anti-CRISPR protein from S. muelleri in mammalian cells

Depositor

Erik Sontheimer

Article

Lee et al MBio. 2018 Dec 4;9(6). pii: mBio.02321-1

Insert

Type II-C anti-CRISPR

Use

CRISPR

Tags

FLAG/NLS

Expression

Mammalian

Promoter

CMV

Available Since

Dec. 17, 2018

Availability

Academic Institutions and Nonprofits only

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HLH Clock Pas 1-401 VenN L74E

Plasmid

#47355

Purpose

Clock fragment mutatation tagged with VenN used for BiFC

Depositor

Joseph Takahashi

Article

Huang et al Science. 2012 Jul 13;337(6091):189-94. d

Insert

Clock (Clock Mouse)

Tags

Venus aa 1-155

Expression

Mammalian

Mutation

aa 1-401, L74E

Promoter

CMV

Available Since

Sept. 4, 2013

Availability

Academic Institutions and Nonprofits only