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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 6,397 results for: kit

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Showing: 1061 - 1080 of 6397 results

  1. pX330a dCas9-KRAB

    Plasmid
    #92361
    Purpose
    Modified CAG promoter-containing vector for ubiquitous expression of catalytically inactive Cas9 fused to KRAB repressor domain. For targeted enhancer inactivation in chicken embryos.
    Depositor
    Tatjana Sauka-Spengler
    Publication
    Williams et al Development. 2018 Feb 23;145(4). pii: de
    Insert
    dCas9-KRAB
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CAG
    Available since
    Aug. 24, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    92361-plasmid-map-sequence-id-178231

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  3. pET30a_HIV-RT

    Plasmid
    #153311
    Purpose
    Bacterial expression of HIV reverse transcriptase
    Depositor
    Bryan Dickinson, Drew Endy
    Insert
    HIV Reverse Transcriptase
    Tags
    His
    Expression
    Bacterial
    Available since
    July 21, 2020
    Availability
    Industry, Academic Institutions, and Nonprofits
    Cited by
    2 Articles
    153311-plasmid-map-sequence-id-306277

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  4. pBUN421

    Plasmid
    #62204
    Purpose
    CRISPR/Cas based plant genome editing and gene regulation; expresses 3×FLAG-NLS-zCas9-NLS, gRNA scaffold for insertion of target sequence (TaU3 promoter), Bar resistance
    Depositor
    Qi-Jun Chen
    Publication
    Xing et al BMC Plant Biol. 2014 Nov 29;14(1):327.
    Inserts
    gRNA scaffold
    zCas9
    Use
    Plant binary vector
    Tags
    3x FLAG and NLS
    Expression
    Plant
    Mutation
    maize codon optimized
    Promoter
    TaU3 and Ubi
    Available since
    Feb. 13, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    62204-plasmid-map-sequence-id-101817

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  5. TtTMPV-Neo

    Plasmid
    #27993
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a TREtight promoter. Contains TurboRFP and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TtTMPV-Neo (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    4 Articles
    27993-plasmid-map-sequence-id-13248

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  11. TRMPV-Neo

    Plasmid
    #27990
    Purpose
    Retroviral transfer plasmid for Tet-regulated shRNA expression with a minimal CMV promoter. Contains DsRed2 and Venus fluorescent reporters.
    Depositor
    Scott Lowe
    Publication
    Zuber et al Nat Biotechnol. 2011 Jan . 29(1):79-83.
    Insert
    TRMPV-Neo (shRen)
    Use
    RNAi and Retroviral; Fluorescent reporters
    Expression
    Mammalian
    Available since
    April 15, 2011
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    27990-plasmid-map-sequence-id-13245

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  12. pG1AK-sfGFP

    Plasmid
    #71739
    Purpose
    Modular shuttle vector for Geobacillus and E. coli
    Depositor
    Tom Ellis
    Publication
    Reeve et al ACS Synth Biol. 2016 Jul 1.
    Insert
    sfGFP
    Use
    Synthetic Biology; Shuttle vector
    Expression
    Bacterial
    Promoter
    pRplsWT
    Available since
    July 5, 2016
    Availability
    Academic Institutions and Nonprofits only
    71739-plasmid-map-sequence-id-130138

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  13. CMVp-dsRed2-Triplex-28-gRNA1-28-pA

    Plasmid
    #55200
    Purpose
    Plasmid encoding the Triplex/gRNA architecture.This is a modified form of the original plasmid described in the paper (Construct 3). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    2 Articles
    55200-plasmid-map-sequence-id-85587

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  15. pDIRECT_21C

    Plasmid
    #91130
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers , Plant Selection: 2x35S:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: 35S:Csy4-P2A-AtCas9 + CmYLCV:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    July 7, 2017
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    1 Article
    91130-plasmid-map-sequence-id-182254

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  16. pDIRECT_25H

    Plasmid
    #91145
    Purpose
    Direct Cloning, Type: T-DNA, Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers, Plant Selection: PvUbi2:hpt II
    Depositor
    Daniel Voytas
    Publication
    Cermak et al Plant Cell. 2017 May 18. pii: tpc.00922.
    Insert
    Engineering Reagent: ZmUbi:Csy4-P2A-TaCas9 + PvUbi1:gRNAs with Csy4 spacers
    Use
    CRISPR
    Expression
    Plant
    Available since
    Sept. 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    91145-plasmid-map-sequence-id-182281

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  17. CMVp-dsRed2-Triplex-28-gRNA3-28-gRNA4-28-gRNA5-28-gRNA6-28-pA

    Plasmid
    #55202
    Purpose
    Plasmid encoding multiplexed 4x gRNAs. This is a modified form of the original plasmid described in the paper (Construct 19). mKate2 was replaced with dsRed2 because of distribution issues.
    Depositor
    Timothy Lu
    Publication
    Nissim et al Mol Cell. 2014 May 14. pii: S1097-2765(1
    Insert
    dsRed2
    Use
    Synthetic Biology
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    June 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    55202-plasmid-map-sequence-id-85589

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Showing: 1061 - 1080 of 6397 results