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pTK_Cerulean_Nanotag_70_neg_Control

Plasmid

#130568

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

negative oligo sequence

Use

Enhancer cloning nanotag reporter vector

Promoter

thymidine kinase minimal promoter

Available Since

Nov. 12, 2019

Availability

Academic Institutions and Nonprofits only

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pCSC-GFP-NES-EGFP

Plasmid

#221810

Purpose

Lentiviral vector expressing green fluorescent protein (GFP) fused with nuclear export sequence (NES).

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

GFP-NES-EGFP

Use

Lentiviral

Tags

NES (internal tag)

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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pABM-D4-miR30-CypA

Plasmid

#132934

Purpose

Lentivector for CypA knockdown and blasticidin resistance

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

CypA shRNA

Use

Lentiviral and RNAi

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV-HA-Ebp1

Plasmid

#67792

Purpose

Mammalian expression of Ebp1 tagged with HA

Depositor

Stephen Goff

Article

Wang et al J Virol. 2014 Feb;88(3):1825-9. doi: 10.

Insert

Ebp1 (PA2G4 Human)

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 1, 2015

Availability

Academic Institutions and Nonprofits only
HyWnt3:Luc

Plasmid

#192986

Purpose

Plasmid to test the Hydra Wnt3 promoter activity

Depositor

Brigitte Galliot

Article

Vogg et al Nat Commun. 2019 Jan 18;10(1):312. doi:

Insert

Hydra Wnt3 promoter

Expression

Mammalian

Available Since

Dec. 2, 2022

Availability

Academic Institutions and Nonprofits only

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pLXIN GFP

Plasmid

#132935

Purpose

MLV-based transfer vector expressing GFP and neomycin resistance protein

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

GFP

Use

Lentiviral, RNAi, and Retroviral

Expression

Mammalian

Promoter

SFFV

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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Mouse ZP3 promoter (JD#445)

Plasmid

#14634

Depositor

Jurrien Dean

Article

Rankin et al Development. 1998 Jul . 125(13):2415-24.

Insert

ZP3 promoter (Zp3 Mouse)

Expression

Bacterial

Available Since

March 23, 2007

Availability

Academic Institutions and Nonprofits only

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pTK-RetE1-Citrine

Plasmid

#127779

Purpose

Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

RetE1

Use

Enhancer reporter construct

Available Since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pCC25

Plasmid

#177319

Purpose

Expression plasmid for production of recombinant T. terrestris CutA NTase fragment in E. coli

Depositor

Rafal Tomecki

Article

Tomecki et al RNA Biol. 2021 Nov 12;18(sup2):623-639.

Insert

TtCutA 240-603

Tags

6xHis-SUMOTag

Expression

Bacterial

Promoter

T7

Available Since

Jan. 28, 2022

Availability

Academic Institutions and Nonprofits only

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pET-MAT2A

Plasmid

#164820

Purpose

Expresses MAT2A in E. coli BL21(DE3)

Depositor

Vern Schramm

Article

Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

Insert

Methionine Adenosyltransferase 2A (MAT2A Human)

Tags

6x His Tag

Expression

Bacterial

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pCSC-NES-RFP

Plasmid

#221812

Purpose

Lentiviral vector expressing red fluorescent protein (RFP) fused with nuclear export sequence (NES)

Depositor

Baojin Ding

Article

Akter et al J Neurosci. 2024 Apr 10;44(15):e17282320

Insert

NES-mCherry

Use

Lentiviral

Tags

NES

Expression

Mammalian

Promoter

CMV

Available Since

July 2, 2024

Availability

Academic Institutions and Nonprofits only

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E12-pCLBabe

Plasmid

#20918

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E12 (TCF3 Human)

Use

Retroviral

Expression

Mammalian

Mutation

G155D

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only

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pmCherry-C1-pmDH

Plasmid

#67897

Purpose

Expresses a fusion of mCherry with the membrane located DH domain (1-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-pmDH

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5 Myc-RIP1

Plasmid

#44159

Depositor

Xin Lin

Article

Blonska et al Mol Cell Biol. 2004 Dec;24(24):10757-65.

Insert

RIP1 (RIPK1 Human)

Tags

Myc

Expression

Mammalian

Available Since

April 18, 2013

Availability

Academic Institutions and Nonprofits only
pPU-huTRIMmiR30-TRIM5

Plasmid

#132938

Purpose

All-in-one huTRIM5 rescue-TRIM5 shRNA knockdown

Depositor

Jeremy Luban

Article

Kim et al Nat Microbiol. 2019 Dec;4(12):2044-2051.

Insert

human TRIM5

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

SFFV

Available Since

Oct. 15, 2019

Availability

Academic Institutions and Nonprofits only

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E47 2/5-pCS2

Plasmid

#20914

Depositor

Stephen Tapscott

Article

Yang et al Genes Dev. 2009 Mar 15. 23(6):694-707.

Insert

E47 2/5 (TCF3 Human)

Expression

Mammalian

Available Since

April 28, 2009

Availability

Academic Institutions and Nonprofits only
pYC3.60-C1

Plasmid

#67899

Purpose

Yellow Cameleon 3.60 in a clontech-style backbone for expression in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

YC3.60

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only
pmVenus-C2-MmMKL2

Plasmid

#67894

Purpose

Expresses a fusion of mVenus with MKL2 (MRTF-B) in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mVenus-MKL2

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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ECFP(W66A)-FRB-MoA

Plasmid

#67904

Purpose

Expresses a Mitochondria located FRB domain in mammalian cells (the ECFP is not fluorescent)

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

ECFP(W66A)-FRB-MoA

Use

Synthetic Biology

Expression

Mammalian

Promoter

CMV

Available Since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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p1195-pssAAV.U1a.hNme2Cas9

Plasmid

#129534

Purpose

AAV vector expressing Nme2Cas9

Depositor

Erik Sontheimer

Article

Lee et al RNA. 2019 Aug 22. pii: rna.071704.119. d

Insert

human codon-optimized Nme2Cas9

Use

AAV

Tags

2xNLS and NLS-3xHA-NLS

Promoter

U1a

Available Since

Sept. 4, 2019

Availability

Academic Institutions and Nonprofits only

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