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pTK_BsmBI_LacZ_Cerulean_Nanotag_66

Plasmid

#130531

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_58

Plasmid

#130527

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 25, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_35

Plasmid

#130524

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_18

Plasmid

#130519

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_17

Plasmid

#130518

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pTK_BsmBI_LacZ_Citrine_Nanotag_11

Plasmid

#130517

Purpose

Enhancer cloning nanotag reporter vector

Depositor

Tatjana Sauka-Spengler

Article

Williams et al Dev Cell. 2019 Oct 21;51(2):255-276.e7.

Insert

LacZ

Use

Enhancer cloning nanotag reporter vector

Tags

Expression

Mutation

Promoter

thymidine kinase minimal promoter

Available since

Sept. 24, 2019

Availability

Academic Institutions and Nonprofits only

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pmCherry-FKBP12-C1-DH

Plasmid

#67901

Purpose

Expresses a fusion of mCherry with FKBP12 and the DH domain (155-347) of p63RhoGEF in mammalian cells

Depositor

Dorus Gadella

Article

van Unen et al Sci Rep. 2015 Oct 5;5:14693. doi: 10.103

Insert

mCherry-FKBP12-DH

Use

Synthetic Biology

Tags

Expression

Mammalian

Mutation

Promoter

CMV

Available since

Jan. 12, 2016

Availability

Academic Institutions and Nonprofits only

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pTK-EdnrbE2-mCherry

Plasmid

#127772

Purpose

Enhancer reporter construct containing the chicken EdnrB enhancer E2 driving mCherry activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

ednrbE2

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pTK-RetE1-Citrine

Plasmid

#127779

Purpose

Enhancer reporter construct containing the chicken RetE1 enhancer driving Citrine activity

Depositor

Tatjana Sauka-Spengler

Article

Ling et al Nat Cell Biol. 2019 Dec;21(12):1504-1517

Insert

RetE1

Use

Enhancer reporter construct

Tags

Expression

Mutation

Promoter

Available since

Jan. 13, 2020

Availability

Academic Institutions and Nonprofits only

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pcDNA3-FLAG/MKRN1(1-280)

Plasmid

#78752

Purpose

To overexpress MKRN1(1-280) in Mammalian Cells

Depositor

Jaewhan Song

Article

Lee et al EMBO J. 2009 Jul 22;28(14):2100-13. doi:

Insert

MKRN1 (MKRN1 Human)

Use

Tags

FLAG

Expression

Mammalian

Mutation

1-280

Promoter

CMV

Available since

June 15, 2016

Availability

Academic Institutions and Nonprofits only

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POC1478

Plasmid

#213804

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1476

Plasmid

#213802

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 16, 2024

Availability

Academic Institutions and Nonprofits only

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POC1477

Plasmid

#213803

Purpose

BsaI-associated switch methylase subunit S

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase S.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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POC1475

Plasmid

#213801

Purpose

BsaI-associated switch methylase subunit M

Depositor

Christopher A. O'Callaghan

Article

Flores-Fernandez et al Appl Microbiol Biotechnol. 2024 Jan 25;1

Insert

BsaI-associated switch methylase M.Sen0738I

Use

Tags

His6-tag

Expression

Bacterial

Mutation

Promoter

T7

Available since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pCMV-WDR5-P2A-SH2-P2A-Ras-CAAX

Plasmid

#209712

Purpose

Mammailan expression of WDR5, SH2, and HRas separated by P2A.

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

WDR5-P2A-SH2-P2A-Ras-CAAX

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV-NLS-MBP-rATOM(WDR5)

Plasmid

#209710

Purpose

Mammalian expression of r-ATOM biosensor for WDR5 with nuclear localization signal

Depositor

Stewart Loh

Article

Sekhon et al Nat Methods. 2023 Nov 9. doi: 10.1038/s4

Insert

NLS-MBP-rATOM(WDR5)

Use

Tags

NLS-MBP

Expression

Mammalian

Mutation

Promoter

Available since

Nov. 2, 2023

Availability

Academic Institutions and Nonprofits only

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pSC-cUnaG

Plasmid

#207631

Purpose

A plasmid encoding photocaged SpyCatcher (pSC) fused to C-terminal UnaG fragment.

Depositor

Cole DeForest

Article

Ruskowitz et al Nat Chem. 2023 May;15(5):694-704. doi: 1

Insert

photocaged SpyCatcher-cUnaG

Use

Tags

6xHis

Expression

Bacterial

Mutation

Amber stop codon at SC's critical lysine

Promoter

T7

Available since

Oct. 5, 2023

Availability

Academic Institutions and Nonprofits only

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ARP3-V5-TurboID

Plasmid

#195144

Purpose

measure COR1C interaction boichemically

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

ACTR3 (ACTR3 Human)

Use

Tags

Expression

Mammalian

Mutation

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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COR1C CC-Halo

Plasmid

#195146

Purpose

image COR1C

Depositor

Gia Voeltz

Article

Striepen et al J Cell Biol. 2022 Aug 1;221(8). pii: 213

Insert

CORO1C (CORO1C Human)

Use

Tags

Expression

Mammalian

Mutation

truncated protein

Promoter

Available since

Sept. 25, 2023

Availability

Academic Institutions and Nonprofits only

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XLone-GvpB

Plasmid

#173798

Purpose

Mammalian acoustic reporter gene 1 cassette 1 (expressing GvpB)

Depositor

Xiaojun Lian

Article

Howells et al Bioeng Transl Med. 2023 Aug 2;9(2):e1058

Insert

GvpB

Use

Tags

mCherry (K187N -please see depositor comments)

Expression

Mammalian

Mutation

Promoter

TRE3G promoter

Available since

July 14, 2023

Availability

Academic Institutions and Nonprofits only

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