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Psicheck2 PTPRN2 full length 3'UTR

Plasmid

#26994

Depositor

Joan Massague

Article

Tavazoie et al Nature. 2008 Jan 10. 451(7175):147-52.

Insert

PTPRN2 Full length 3' UTR (PTPRN2 Human)

Use

Luciferase

Tags

Renilla Luciferase

Expression

Mammalian

Available Since

Jan. 11, 2011

Availability

Academic Institutions and Nonprofits only
pCDNA5/FRT-ChemoC

Plasmid

#193809

Purpose

CMV overexpression of ChemoC in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoC

Tags

10xHis

Expression

Mammalian

Mutation

mCerulean3:T225R; HT7: E143R, E147R, L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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pPANI2A2E2-Crimson

Plasmid

#193758

Purpose

Expresses E2-Crimson under the control of PANI2 synthetic promoter, ColE1 origin of replication, Ampicillin selection

Depositor

Sangram Bagh

Article

Sarkar et al Chem Sci. 2021 Nov 9;12(48):15821-15832.

Insert

E2-Crimson

Use

Synthetic Biology

Expression

Bacterial

Promoter

PANI2 synthetic promoter carrying binding sites f…

Available Since

April 7, 2023

Availability

Academic Institutions and Nonprofits only

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pCI pHluorin Syt10

Plasmid

#184511

Purpose

Mammalian expression of Synaptotagmin 10 with pH-sensitive fluorescent protein

Depositor

Edwin Chapman

Article

Dean et al Mol Biol Cell. 2012 May;23(9):1715-27. d

Insert

Synaptotagmin 10 (Syt10 Mouse)

Tags

Super Ecliptic pHluorin

Expression

Mammalian

Available Since

May 2, 2023

Availability

Academic Institutions and Nonprofits only

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12URA-B-ScTop2∆CTD

Plasmid

#214924

Purpose

Yeast expression vector 6xHisTag-TEV at N terminus to express S cerevisiae topoisomerase II (1–1177)

Depositor

James Berger

Article

Jeong et al Elife. 2022 Nov 7;11:e81786. doi: 10.755

Insert

topoisomerase II

Tags

6x His-TEV

Expression

Yeast

Mutation

Deleted amino acids 1178-1428

Promoter

Gal 1/10

Available Since

March 28, 2024

Availability

Academic Institutions and Nonprofits only

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pLX-UBC_NKX2-1_Short_V5-GFP

Plasmid

#221499

Purpose

NKX2-1-GFP fusion protein lentiviral overexpression using an UBC promoter

Depositor

Matthew Meyerson

Article

Pulice et al bioRxiv [Preprint]. 2023 Oct 27:2023.10.

Insert

NKX2-1 (NKX2-1 Human)

Use

Lentiviral

Tags

V5-linker-GFP

Expression

Mammalian

Promoter

UBC

Available Since

July 1, 2024

Availability

Academic Institutions and Nonprofits only

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pLX307 hcRed1

Plasmid

#117732

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-mCherry-2xEcMTS

Plasmid

#133624

Purpose

Expression vector for mCherry fused to a tandem repeat of the E. coli MinD membrane targeting sequence (MTS) (C-terminal fusion) and an N-terminal His-tag for purification.

Depositor

Petra Schwille

Article

Ramm et al Nat Commun. 2018 Sep 26;9(1):3942. doi:

Insert

mCherry-2xEcMTS

Tags

His

Expression

Bacterial

Available Since

Feb. 4, 2020

Availability

Academic Institutions and Nonprofits only

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Ppst93-ANME2bHR1 MCR-NG

Plasmid

#192774

Purpose

Expresses NG-tagged ANME2bHR1 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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Ppst93-ANME1G37 MCR-NG

Plasmid

#192773

Purpose

Expresses NG-tagged ANME1G37 MCR in Methanococcus maripaludis

Depositor

William B. Whitman

Article

Shao et al Commun Biol. 2022 Oct 20;5(1):1113. doi:

Insert

Methyl coenzyme M reductase

Use

Synthetic Biology; Heterologous expression in met…

Tags

Flag-Strep2

Available Since

Dec. 7, 2022

Availability

Industry, Academic Institutions, and Nonprofits

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pCDNA5/FRT-ChemoD-NAD

Plasmid

#193826

Purpose

CMV overexpression of ChemoD-NAD in mammalian cell lines

Depositor

Julien Hiblot

Article

Hellweg et al Nat Chem Biol. 2023 Jun 8. doi: 10.1038/

Insert

ChemoD-NAD

Expression

Mammalian

Mutation

ShadowG: T225R, HT7: L271E

Promoter

CMV

Available Since

June 9, 2023

Availability

Academic Institutions and Nonprofits only

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Aurli_150841_GZG

Plasmid

#162563

Purpose

Inactivation of Aurli_150841 (crtIBY) in Aurantiochytrium limacinum MYA-1381 with zeocin resistance driven by native GAPDH promoter/termiator system.

Depositor

Jackie Collier

Article

Rius et al Genome Biol Evol. 2023 Mar 3;15(3):evad0

Insert

shble

Use

Unspecified

Promoter

GAPDH

Available Since

May 28, 2024

Availability

Academic Institutions and Nonprofits only

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pVH231-Tier1-PmPGK1-mTagBFP2

Plasmid

#169520

Purpose

Tier-1 vector encoding PmPGK1-driven mTagBFP2 expression (PmPGK1-mTagBFP2-pA).

Depositor

Martin Fussenegger

Article

Haellman et al Metab Eng. 2021 Jul;66:41-50. doi: 10.10

Insert

PPGK-driven monomeric Tag blue fluorescent protein 2

Expression

Mammalian

Promoter

PPGK

Available Since

June 16, 2022

Availability

Academic Institutions and Nonprofits only

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pLX311 hcRed1

Plasmid

#117733

Purpose

Open reading frame vector encoding hcRed1

Depositor

William Hahn

Article

Hong et al Mol Cancer Res. 2019 Feb 18. pii: 1541-7

Insert

hcRed1

Use

Lentiviral

Tags

V5

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 21, 2019

Availability

Academic Institutions and Nonprofits only

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pCsC_spacer

Plasmid

#136077

Purpose

Use when making odd level constructs (L3,...) if pCsC position (position 3) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE_spacer

Plasmid

#136078

Purpose

Use when making odd level constructs (L3,...) if pCsE position (position 4) has no construct assigned

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

Even spacer

Use

Synthetic Biology

Expression

Plant

Mutation

BsaI/ SapI domesticated

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsB

Plasmid

#136068

Purpose

Acceptor plasmid for even level position 2, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsC

Plasmid

#136069

Purpose

Acceptor plasmid for even level position 3, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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pCsE

Plasmid

#136070

Purpose

Acceptor plasmid for even level position 4, lacZ blue-white screening

Depositor

Jim Haseloff

Article

Sauret-Gueto et al ACS Synth Biol. 2020 Mar 12. doi: 10.102

Insert

lacZ

Use

Synthetic Biology

Expression

Plant

Available Since

Nov. 30, 2021

Availability

Industry, Academic Institutions, and Nonprofits

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Lv EF1a HP1cs-Frb1x PGK Puro

Plasmid

#102808

Purpose

Lentiviral plasmid from FIRE-Cas9 system to express HP1cs-Frb1x fusion protein

Depositor

Jerry Crabtree

Article

Braun et al Nat Commun. 2017 Sep 15;8(1):560. doi: 1

Insert

HP1cs-Frb1x

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

EF1a

Available Since

Feb. 9, 2018

Availability

Academic Institutions and Nonprofits only

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