We narrowed to 11,592 results for: Vars;

1. PB-PEmax NG

   Plasmid

   #187894

   Purpose

   All-in-one prime editor piggyBac transposon with PEmax, NG variant

   Depositor

   Tobias Cantz

   Article

   Eggenschwiler et al Sci Rep. 2021 Nov 12;11(1):22154. doi: 1

   Insert

   SpCas9_H840A_KK_NG-MMLV-RT_co-P2A-PAC_dTK
   

   Use

   CRISPR and Synthetic Biology; Piggybac transposon

   Tags

   BPSV40 NLS and c-Myc NLS

   Expression

   Mammalian

   Promoter

   CAG

   Available Since

   Aug. 31, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. TFORF0926

   Plasmid

   #143240

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   ATOH7 (ATOH7 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   May 10, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pAAV-hSyn-DIO {hCAR}off-{ChETA}on-WPRE

   Plasmid

   #111388

   Purpose

   AAV vector with hSynapsin promoter, Cre-OFF hCAR (for efficient CAV-2 infection) and Cre-ON ChETA (for optogenetic activation)

   Depositor

   Adam Kepecs

   Article

   Li et al Neuron. 2018 Jun 6;98(5):905-917.e5. doi

   Inserts

   hCAR (CXADR Human)
   ChETA
   

   Use

   AAV

   Tags

   HA and Myc

   Expression

   Mammalian

   Promoter

   hSynapsin

   Available Since

   June 7, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. plx307 SHOC2 V5 WT

   Plasmid

   #190568

   Purpose

   SHOC2 variant overexpression

   Depositor

   William Hahn

   Article

   Kwon et al Nature. 2022 Jul 13. pii: 10.1038/s41586

   Insert

   SHOC2 (SHOC2 Human)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Nov. 2, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. p2R3a-VenusYFP-3AT

   Plasmid

   #71269

   Purpose

   Entry clone containing Venus. Can be fused with linker to the C-terminal end of protein of interest. For use in plants and compatible with the MultiSite Gateway system

   Depositor

   Ari Pekka Mähönen

   Article

   Siligato et al Plant Physiol. 2015 Dec 7. pii: pp.01246

   Insert

   VenusYFP
   

   Use

   Gateway

   Tags

   4xGly linker and T3A pea Pisum sativum ribulose-1…

   Available Since

   Dec. 7, 2015

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. pAAV-EF1α1.1-tdTomato

   Plasmid

   #122101

   Purpose

   AAV-mediated expression of tdTomato under the EF1α promoter (1.1kb short version). tdTomato has codons varied to reduce recombination. Using SV40 pA signal.

   Depositor

   Edward Boyden

   Article

   Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

   Insert

   tdTomato
   

   Use

   AAV

   Tags

   NA

   Expression

   Mammalian

   Promoter

   EF1a（1.1kb short version)

   Available Since

   June 15, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pTH733-2µ-RLuc/maxCFLuc

   Plasmid

   #40608

   Depositor

   Tobias von der Haar

   Article

   Chu et al EMBO J. 2014 Jan 1;33(1):21-34. doi: 10.

   Inserts

   Firefly Luciferase
   Renilla luciferase
   

   Expression

   Yeast

   Mutation

   All codons of the original FLuc gene were exchang…

   Promoter

   ADH1 and TDH3

   Available Since

   Oct. 30, 2012

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pTwist-SARS-CoV-2 Spike-deltaC18 BA.2

   Plasmid

   #212487

   Purpose

   Encodes SARS-CoV-2 variant BA.2 Spike for pseudovirus production

   Depositor

   Alejandro Balazs

   Article

   Roederer et al medRxiv [Preprint]. 2024 Mar 7:2024.03.0

   Insert

   SARS-CoV-2 Spike BA.2 (S Severe acute respiratory syndrome coronavirus 2)
   

   Expression

   Mammalian

   Mutation

   See Depositor Comments.

   Promoter

   CAG

   Available Since

   Sept. 13, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pAAV-ITR-PytR(PyOtR)-mCherry

   Plasmid

   #204870

   Purpose

   Expresses pyrrolysyl tRNA variant "PyOtR" and a wild-type mCherry reporter; can be packaged into AAV

   Depositor

   Abhishek Chatterjee

   Article

   Jewel et al Angew Chem Int Ed Engl. 2024 Jan 26:e202

   Insert

   M. mazei pyrrolysyl tRNA mutant "PyOtR"
   

   Use

   AAV

   Expression

   Mammalian

   Mutation

   U25C; A3G, A4C, A5G, C6G, G63C, T65G, T66C

   Promoter

   U6

   Available Since

   Jan. 30, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pENTR1A mKate2-hu_ncOGT K842M

    Plasmid

    #154284

    Purpose

    Entry vector encoding mKate2-2xFLAG-OGT (O-GlcNAc Transferase; K842M variant, catalytic dead)

    Depositor

    Suzanne Walker

    Article

    Levine et al Proc Natl Acad Sci U S A. 2021 Jan 26;11

    Insert

    O-GlcNAc Transferase (OGT Human)
    

    Use

    Gateway entry vector

    Tags

    2x FLAG and mKate2

    Mutation

    K842M mutation (Catalytic Inactive)

    Available Since

    Nov. 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pLEX304-GFP-v1 IQSEC1 WT

    Plasmid

    #162016

    Purpose

    Expression of IQSEC1 splice variant 1

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pAAV-syn-jYCaMP1s

    Plasmid

    #135424

    Purpose

    Yellow protein calcium sensor expressed under human Synapsin1 promoter, for AAV production or direct transfection, slow variant

    Depositor

    Kaspar Podgorski

    Article

    Mohr et al Nat Methods. 2020 May 25. pii: 10.1038/s

    Has Service

    AAV1

    Insert

    jYCaMP1s
    

    Use

    AAV

    Expression

    Mammalian

    Promoter

    hSyn

    Available Since

    Jan. 8, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3.1-V5H6-mPCSK3 D153A

    Plasmid

    #122681

    Purpose

    Expresses mutated mouse PCSK3 in mammalian cells (aspartic acid at position 153 mutated to alanine via site directed mutagenesis)

    Depositor

    Liming Pei

    Article

    Li et al Mol Cell Biol. 2018 Oct 15;38(21). pii:

    Insert

    Mus musculus furin (paired basic amino acid cleaving enzyme) (Furin), transcript variant 1 (Furin Mouse)
    

    Expression

    Mammalian

    Mutation

    changed Aspartic Acid 153 to Alanine

    Available Since

    May 16, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. pLEX304-GFP-v2 IQSEC1 WT

    Plasmid

    #162017

    Purpose

    Expression of IQSEC1 splice variant 2

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pαH-S-HRV3C

    Plasmid

    #164567

    Purpose

    SARS-CoV-2 Spike protein with furin site mutated to HRV3C site (S-HRV3C variant)

    Depositor

    Priyamvada Acharya

    Article

    Gobeil et al Cell Rep. 2021 Jan 12;34(2):108630. doi:

    Insert

    Spike - Furin site changed for HRV3C site
    

    Tags

    2X Strep-Tag II and 8X His tag

    Expression

    Mammalian

    Mutation

    682-685 (furin site) replaced with HRV3C site (LE…

    Available Since

    Feb. 26, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pcDNA-GFP-EphrinB3-Flag(A227Y)

    Plasmid

    #155013

    Purpose

    Expresses N-terminally GFP-tagged and C-terminally Flag-tagged EphrinB3 A227Y variant from pcDNA3.1

    Depositor

    Sinisa Urban

    Article

    Gandhi et al Cell Chem Biol. 2020 Aug 31. pii: S2451-

    Insert

    EphrinB3 (EPHB3 Human)
    

    Tags

    Flag, GFP, and signal peptide (residues 1 to 32) …

    Expression

    Mammalian

    Mutation

    A227Y

    Promoter

    CMV

    Available Since

    Nov. 2, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. LIC1B_Caspase-LOVN7C-7 C450A

    Plasmid

    #104628

    Purpose

    bacterial expression of light-activated caspase-3 with N7C-7 linker variation on C450A mutation

    Depositor

    Jim Wells

    Article

    Smart et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

    Insert

    Caspase-3 (CASP3 Human)
    

    Tags

    His and LOV2

    Expression

    Bacterial

    Mutation

    C450A (dark-locked and light-insensitive mutation)

    Promoter

    T7

    Available Since

    April 9, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLEX304-GFP-v3 IQSEC1 WT

    Plasmid

    #162018

    Purpose

    Expression of IQSEC1 splice variant 3

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    April 5, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pLEX304-GFP-v4 IQSEC1 WT

    Plasmid

    #162019

    Purpose

    Expression of IQSEC1 splice variant 4

    Depositor

    David Bryant

    Article

    Nacke et al Nat Commun. 2021 Mar 12;12(1):1623. doi:

    Insert

    IQSEC1 (IQSEC1 Human)
    

    Use

    Lentiviral

    Available Since

    Dec. 7, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. plenti-UbcP-IKZF1-V2-3xHA-pGK-Pur

    Plasmid

    #69051

    Purpose

    lentiviral expression of IKZF1 with HA tag

    Depositor

    William Kaelin

    Article

    Lu et al Science. 2014 Jan 17;343(6168):305-9. do

    Insert

    IKZF1 (IKZF1 Human)
    

    Use

    Lentiviral

    Tags

    HA

    Expression

    Mammalian

    Mutation

    splice variant 2

    Promoter

    UBC

    Available Since

    Nov. 1, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge