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p3E-Rac1-DN

Plasmid

#109582

Purpose

Multisite gateway vector for 3' tagging with dominant negative Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Mutation

dominant negative

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pCDNA3.1neo-14-3-3-CLuc

Plasmid

#107611

Purpose

measure LATS activity by Luciferase Assay

Depositor

Xiaolong Yang

Article

Azad et al Nat Commun. 2018 Mar 13;9(1):1061. doi:

Insert

pCDNA3.1hygro-BamHI-CLuc1433-NotI

Expression

Mammalian

Available Since

May 21, 2018

Availability

Academic Institutions and Nonprofits only

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pEY121

Plasmid

#223468

Purpose

sra-6 fluorescent neural reporter driving nuclear mTagBFP2 expression (refer to NeuroPAL paper for expression)

Depositor

Oliver Hobert, Eviatar Yemini

Article

Yemini et al Cell. 2021 Jan 7;184(1):272-288.e11. doi

Insert

sra-6 (sra-6 Nematode)

Expression

Worm

Available Since

Aug. 19, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin4B

Plasmid

#41915

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL4B (CUL4B Human)

Use

Lentiviral

Available Since

April 3, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn-DIO-hM3D(Gq)-mCherry-shLacZ-CWB

Plasmid

#223660

Purpose

Expresses hM3D(Gq) and a control shRNA (targetting LacZ) in a Cre-dependent manner

Depositor

Oscar Marin

Article

Selten et al Nature. 2025 Apr 30. doi: 10.1038/s41586

Insert

hM3D(Gq)-mCherry and shLacZ

Use

AAV and RNAi

Tags

mCherry

Expression

Mammalian

Promoter

hSyn

Available Since

April 30, 2025

Availability

Academic Institutions and Nonprofits only

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pLenti-CMV-cGASΔN-HA

Plasmid

#130912

Purpose

Expresses human cGASΔN (aa160-522)-HA; Puromycin selection marker

Depositor

Jonathan Kagan

Article

Barnett et al Cell. 2019 Mar 7;176(6):1432-1446.e11. d

Insert

cGASΔN

Tags

HA

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 27, 2019

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+)-MC38-TCRA-33-MC_hammer

Plasmid

#138989

Purpose

IVT template for the alpha subunit of the mouse TCR #33 that is reactive against MC38

Depositor

Jeff Hammerbacher

Article

Gottschalk et al bioRxiv 2021.11.30.470597

Insert

TCRA (Tcra Mouse)

Expression

Mammalian

Promoter

CMV and T7

Available Since

March 9, 2020

Availability

Academic Institutions and Nonprofits only

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pPagP

Plasmid

#112125

Purpose

Bacterial expression of outer membrane protein PagP, wild type

Depositor

Karen Fleming

Article

Burgess et al J Biol Chem. 2008 Sep 26;283(39):26748-5

Insert

PagP (pagP E. coli)

Expression

Bacterial

Mutation

N-terminal signal sequence not included and repla…

Promoter

T7

Available Since

Nov. 8, 2019

Availability

Academic Institutions and Nonprofits only

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pGEL063

Plasmid

#124895

Purpose

For genome editing in plant by SpCas9 variant(SpCas9-NG)

Depositor

Yong Zhang

Article

Zhong et al Mol Plant. 2019 Mar 27. pii: S1674-2052(

Type

Empty backbone

Use

CRISPR

Expression

Plant

Available Since

Jan. 9, 2020

Availability

Academic Institutions and Nonprofits only

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p15aC-4D1D-5

Plasmid

#107866

Purpose

Bacterial expression of PIS, PI4K and PI4P5K

Depositor

Sanford Simon

Article

Botero et al Sci Adv. 2019 Mar 27;5(3):eaat4872. doi:

Inserts

PIS
phosphatidylinositol 4-phosphate 5-kinase
phosphatidylinositol 4-kinase β

Tags

myc

Expression

Bacterial

Promoter

proD and proD (in operon)

Available Since

April 8, 2019

Availability

Academic Institutions and Nonprofits only

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pET28a-VcRibB

Plasmid

#187977

Purpose

Expresses 3,4-dihydroxy-2-butanone 4-phosphate synthase RibB from Vibrio cholerae

Depositor

Audrey Lamb

Article

Kenjic et al J Am Chem Soc. 2022 Jul 20;144(28):12769

Insert

VcRibB

Tags

Hexahistag

Expression

Bacterial

Promoter

T7

Available Since

March 18, 2024

Availability

Academic Institutions and Nonprofits only

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pHAGE DN Cullin1

Plasmid

#41911

Depositor

Stephen Elledge

Article

Emanuele et al Cell. 2011 Oct 14;147(2):459-74. doi: 10

Insert

CUL1 (CUL1 Human)

Use

Lentiviral

Promoter

CMV

Available Since

April 17, 2013

Availability

Academic Institutions and Nonprofits only

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pDGE342

Plasmid

#153251

Purpose

M8E shuttle vector containing the Arabidopsis thaliana U6 promoter for preparation of single guide RNA transcriptional units by cloning of hybridized oligonucleotides.

Depositor

Johannes Stuttmann

Article

Stuttmann et al Plant J. 2021 Feb 12. doi: 10.1111/tpj.1

Type

Empty backbone

Use

CRISPR

Available Since

Aug. 6, 2020

Availability

Academic Institutions and Nonprofits only

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pAAV-2Aneo v2

Plasmid

#80033

Purpose

Used to construct AAV-based targeting vectors that carry an IVS-2Aneo cassette for promoter-trapping

Depositor

Hiroyuki Konishi

Article

Karnan et al Nucleic Acids Res. 2015 Dec 10. pii: gkv

Type

Empty backbone

Use

AAV and Cre/Lox; A platform vector to construct t…

Available Since

Aug. 3, 2016

Availability

Academic Institutions and Nonprofits only

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pRK5-mFzd2

Plasmid

#42254

Depositor

Chris Garcia, Jeremy Nathans

Article

Yu et al Development. 2012 Dec 1;139(23):4383-94.

Insert

Frizzled 2 (Fzd2 Mouse)

Expression

Mammalian

Available Since

April 24, 2013

Availability

Academic Institutions and Nonprofits only

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pAAV-hSyn1-2xNLS-mTurquoise2

Plasmid

#118025

Purpose

An AAV genome that expresses nuclear localized mTurquoise2 from the hSyn1 promoter

Depositor

Viviana Gradinaru

Article

Challis et al Nat Protoc. 2019 Feb;14(2):379-414. doi:

Insert

2xNLS-mTurquoise2

Use

AAV

Tags

NLS

Promoter

hSyn1

Available Since

Nov. 28, 2018

Availability

Academic Institutions and Nonprofits only

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pTRE3G-iRFP-B1-timestamp_A

Plasmid

#159924

Purpose

Timestamp Template A inserted into the 3’ UTR of iRFP between bActin Zipcode element and WPRE, with RNA barcode TGC. Also includes a xrRNA element in the 5’ UTR. AKA 147B1.

Depositor

Fei Chen

Article

Rodriques et al Nat Biotechnol. 2020 Oct 19. pii: 10.103

Insert

iRFP-Timestamp A

Expression

Mammalian

Promoter

TRE3G

Available Since

Dec. 2, 2020

Availability

Academic Institutions and Nonprofits only

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p3E-Rac1

Plasmid

#109581

Purpose

Multisite gateway vector for 3' tagging with Rac1

Depositor

Rob Parton

Article

Hall et al Nat Commun. 2020 Jul 24;11(1):3711. doi:

Insert

Rac1 (rac1a Zebrafish)

Use

Zebrafish plasmids

Available Since

Feb. 27, 2020

Availability

Academic Institutions and Nonprofits only

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pMy-CTCF-GFP

Plasmid

#50574

Purpose

Retroviral vector endoding for mouse CTCF fused to GFP

Depositor

Rafael Casellas

Article

Nakahashi et al Cell Rep. 2013 May 30;3(5):1678-89. doi:

Insert

Ampicilin

Use

Retroviral

Available Since

Feb. 23, 2015

Availability

Academic Institutions and Nonprofits only

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p3xFlag-CMV7-Erk2

Plasmid

#39223

Depositor

Melanie Cobb

Article

Robinson et al J Biol Chem. 2002 Apr 26;277(17):14844-5

Insert

Erk2 (Mapk1 Rat)

Tags

3xFLAG

Expression

Mammalian

Promoter

CMV

Available Since

July 15, 2013

Availability

Academic Institutions and Nonprofits only

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