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pJS110

Plasmid

#207591

Purpose

GFP-AIA H22A expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-AIA H22A

Expression

Bacterial

Available Since

Nov. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pJMG1

Plasmid

#207594

Purpose

GFP-TonB deltaPII expression

Depositor

Colin Kleanthous

Article

Williams-Jones et al Proc Natl Acad Sci U S A. 2023 Nov 21;12

Insert

GFP-TonB deltaPII

Expression

Bacterial

Available Since

Nov. 13, 2023

Availability

Academic Institutions and Nonprofits only

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pRSF- RPA4 (MSW#716)

Plasmid

#208077

Purpose

Expression of human Replication Protein A 4 in E.coli

Depositor

Marc Wold

Article

Mason et al J Biol Chem. 2009 Feb 20;284(8):5324-31.

Insert

RPA4 (RPA4 Human)

Expression

Bacterial

Available Since

Nov. 1, 2023

Availability

Academic Institutions and Nonprofits only

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pRegev_ExonSkip_Backbone

Plasmid

#204187

Purpose

Backbone for cloning splicing library with truncated CAG promoter and first half of beta globin minigene reporter (exon 1 and intron 1).

Depositor

Oded Regev

Article

Liao et al Proc Natl Acad Sci U S A. 2023 Oct 10;12

Insert

First exon and intron for beta globin splicing minigene

Expression

Mammalian

Promoter

Truncated CAG

Available Since

Aug. 14, 2023

Availability

Academic Institutions and Nonprofits only

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pHACK-QF2-AgU6-mCherry

Plasmid

#184530

Purpose

HACK-compatible plasmid for generating T2A-QF2 knockins in Anopheles mosquitoes with a mCherry selectable marker.

Depositor

Christopher Potter

Article

Raji et al Cell Rep. 2023 Feb 10;42(2):112101. doi:

Insert

QF2

Use

CRISPR

Promoter

Anopheles U6 promoter

Available Since

July 5, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-dCer3-C2-FRB

Plasmid

#191327

Purpose

Expresses the fluorescently labeled membrane anchor C2 fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-C2-dCer3

Expression

Mammalian

Available Since

June 7, 2023

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1_ E3_5 _hFc

Plasmid

#199617

Purpose

Encodes DARPin-hFc E3_5 for mammalian expression and column purification

Depositor

Shiva Tyagarajan

Article

Campbell et al Elife. 2022 Oct 31;11:e80895. doi: 10.75

Insert

DARPin-hFc E3_5 (control)

Tags

hFc

Expression

Mammalian

Promoter

CMV

Available Since

May 1, 2023

Availability

Academic Institutions and Nonprofits only

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SP_H6_Halo_WT_KDEL_pBABEpu

Plasmid

#183688

Purpose

Retroviral expression of ER targeted H6-tagged Halotag Wild type

Depositor

Edward Avezov

Article

Melo et al Nat Commun. 2022 May 6;13(1):2501. doi:

Insert

ER Halo

Use

Retroviral

Available Since

April 14, 2023

Availability

Academic Institutions and Nonprofits only

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pL8N_RB610-HBB_X1

Plasmid

#191521

Purpose

Expression of nuclear ZF-DdCBE in mammalian cells (N-terminal DddAC, Right)

Depositor

David Liu

Article

Willis et al Nat Commun. 2022 Nov 23;13(1):7204. doi:

Insert

ZF-DdCBE RB610-HBB X1

Expression

Mammalian

Promoter

CMV

Available Since

April 11, 2023

Availability

Academic Institutions and Nonprofits only

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psiCHECK-EGFP

Plasmid

#196606

Purpose

Dual-Luciferase Reporter vector carrying an EGFP direct sequence

Depositor

Franco Lucchini

Article

Villa et al Virology. 2022 Dec 31;579:38-45. doi: 10

Insert

GFP

Expression

Mammalian

Available Since

March 29, 2023

Availability

Academic Institutions and Nonprofits only

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pCMV128-Rluc-CTE-GAG_O

Plasmid

#147108

Purpose

Mammalian Expression of Rluc-CTE-GAG

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

Rluc-CTE-GAG

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pLNHA-C1-HIV-Rev-M10_O

Plasmid

#147112

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pT7-EGFP-C1-HIV-Rev-M10_O

Plasmid

#147114

Purpose

Mammalian Expression of HIV-Rev-M10

Depositor

Elisa Izaurralde

Article

Teplova et al Nat Struct Mol Biol. 2011 Aug 7;18(9):99

Insert

HIV-Rev-M10

Expression

Mammalian

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pAc5.1C-Fluc-CG10596_E

Plasmid

#146189

Purpose

Insect Expression of CG10596-3UTR

Depositor

Elisa Izaurralde

Article

Eulalio et al Genes Dev. 2007 Oct 15. 21(20):2558-70.

Insert

CG10596-3UTR (Msr-110 Fly)

Expression

Insect

Available Since

March 16, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-FRB-dCer3-CAAX

Plasmid

#191326

Purpose

Expresses the fluorescently labeled membrane anchor CAAX fused to the dimerization domain FRB to recruit proteins to plasma membrane by using the chemical dimerization system FKBP-FRB

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

FRB-dCer-CAAX

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pBetaActin-TUBB2a-mEos3.2

Plasmid

#191320

Purpose

Expresses fuorescently labeled beta tubulin to allow photoconversion of microtubule patches from green to red fluorescent with UV light

Depositor

Frank Bradke

Article

Schelski et al Sci Adv. 2022 Nov 4;8(44):eabo2336. doi:

Insert

TUBB2a-mEos3.2 (Tubb2a Mouse)

Expression

Mammalian

Available Since

March 15, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-345

Plasmid

#195456

Purpose

Lentiviral Integratable, constitutive expression of synZiFTR: ZF1-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - 3XFLAG - NLS - ZF1 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMZ-346

Plasmid

#195457

Purpose

Lentiviral Integratable, constitutive expression of synZiFTR: ZF3-p65

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

pSFFV - 3XFLAG - NLS - ZF1 - p65

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-258

Plasmid

#195438

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF1

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF1 BS - pSFFV - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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pMN-259

Plasmid

#195439

Purpose

Lentiviral Integratable mCherry Reporter for synZiFTR ZF3

Depositor

Ahmad Khalil

Article

Li et al Science. 2022 Dec 16;378(6625):1227-1234

Insert

4X ZF3 BS - pSFFV - mCherry

Use

Lentiviral

Expression

Mammalian

Available Since

March 3, 2023

Availability

Academic Institutions and Nonprofits only

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