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pKK-mCerulean-TEV

Plasmid

#105773

Purpose

Expression of your protein of interest in fusion with cyan fluorescent protein at the N-terminus (cleavable by TEV). Useful for FRET experiments (PMID: 14990965, 17040988, 22264545).

Depositor

Andrzej Dziembowski

Article

Szczesny et al PLoS One. 2018 Mar 28;13(3):e0194887. do

Type

Empty backbone

Use

Flp-in competent

Tags

mCerulean-TEV

Expression

Mammalian

Available Since

Feb. 12, 2018

Availability

Academic Institutions and Nonprofits only

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KLHL31 pgk (1088)

Plasmid

#62031

Purpose

mammalian expression of nuclear envelope transmembrane protein

Depositor

Eric Schirmer

Article

Wilkie et al Mol Cell Proteomics. 2011 Jan;10(1):M110

Insert

KLHL31 (Klhl31 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 17, 2015

Availability

Academic Institutions and Nonprofits only
pCAGEN-FastFUCCI-IRES-myr-mTagBFP (JDW 1502)

Plasmid

#242597

Purpose

A CAGGS driven expression vector containing FastFUCCI to label cell cycle state followed by an IRES and a membrane targeted mTagBFP2

Depositor

Joshua Wythe

Article

Gillespie WB et al Development. 2025 Nov 15;152(22):dev2043

Insert

mKO2-hCDT1(30-120) T2A mAG-hGEM(1-110)

Expression

Mammalian

Promoter

CAGGS

Available Since

Dec. 16, 2025

Availability

Academic Institutions and Nonprofits only

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HisGb1-STAT1(710-750) + CBP TAZ2 (1764-1855)

Plasmid

#99342

Purpose

Bacterial expression of mouse CBP TAZ2 domain by coexpression with HisGB1 tagged STAT1 TAD

Depositor

Peter Wright

Article

Wojciak et al EMBO J. 2009 Apr 8;28(7):948-58. doi: 10

Inserts

STAT1 (710-750) (STAT1 Human)
CBP (1764-1855)

Tags

His6GB1

Expression

Bacterial

Promoter

T7

Available Since

Sept. 13, 2017

Availability

Academic Institutions and Nonprofits only

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10_T7-SP-CD8tm-mScarlet3

Plasmid

#225937

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with a signal peptide and the CD8 transmembrane domain

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

SP-CD8tm-mScarlet3

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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9_T7-mScarlet3-LactC2

Plasmid

#225936

Purpose

In vitro synthesis of mRNA for fluorescent protein mScarlet3 tagged with the LactC2 membrane localisation signal

Depositor

Michalis Averof

Article

Karapidaki et al Development. 2026 Apr 15;153(8):dev20541

Insert

mScarlet3-LactC2

Use

In vitro transcription

Promoter

T7

Available Since

Nov. 11, 2024

Availability

Academic Institutions and Nonprofits only

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TRPML1-mCherry

Plasmid

#135189

Purpose

Expresses TRPML1 with C-terminus mCherry tag in mammalian cells.

Depositor

Antony Galione

Article

Ruas et al EMBO J. 2015 Jul 2;34(13):1743-58. doi:

Insert

TRPML1 (Mcoln1 Mouse)

Use

Lentiviral

Expression

Mammalian

Available Since

Dec. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pAAV-UBC-FLEX-XRI-FLAG

Plasmid

#178057

Purpose

Expression Recording Islands (XRIs) for recording cellular physiological histories along intracellular protein self-assembly. Contains XRI subunit with FLAG tag, under UBC promoter with a FLEX switch.

Depositor

Edward Boyden

Article

Linghu et al Nat Biotechnol. 2023 Jan 2. doi: 10.1038

Insert

XRI-FLAG

Use

AAV

Tags

FLAG-MBP

Expression

Mammalian

Promoter

Human ubiquitin C (UBC) promoter

Available Since

Oct. 9, 2022

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf6(WT)-mCherry

Plasmid

#79422

Purpose

Expresses C-terminally mCherry-tagged Arf6(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf6 (ARF6 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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pcDNA3/hArf1(WT)-mCherry

Plasmid

#79419

Purpose

Expresses C-terminally mCherry-tagged Arf1(WT) in mammalian cells

Depositor

Kazuhisa Nakayama

Article

Makyio et al EMBO J. 2012 May 30;31(11):2590-603. doi

Insert

Arf1 (ARF1 Human)

Tags

mCherry

Expression

Mammalian

Available Since

April 11, 2017

Availability

Academic Institutions and Nonprofits only

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