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mKO2-N1

Plasmid

#54625

Purpose

Localization: N1 Cloning Vector, Excitation: 551, Emission: 565

Depositor

Michael Davidson, Atsushi Miyawaki

Article

Sakaue-Sawano et al Cell. 2008 Feb 8;132(3):487-98. doi: 10.

Type

Empty backbone

Tags

mKO2

Expression

Mammalian

Available Since

June 12, 2014

Availability

Academic Institutions and Nonprofits only

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pMOS016: roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Plasmid

#163058

Purpose

roGFP2-Orp1 H2O2 oxidation sensor (cytosolic)

Depositor

Adam Cohen

Article

Werley et al Nat Commun. 2020 Aug 4;11(1):3881. doi:

Insert

roGFP2-Orp1

Use

Lentiviral

Expression

Mammalian

Promoter

CMV

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pOVC1_RsLP-LOV_X

Plasmid

#131177

Purpose

Expresses RsLP-LOV (Rhodobacter sphaeroides) followed by restriction sites

Depositor

Harald Janovjak

Article

Tichy et al J Mol Biol. 2019 May 29. pii: S0022-2836

Insert

RsLP-LOV

Expression

Mammalian

Promoter

CMV

Available Since

Sept. 17, 2019

Availability

Academic Institutions and Nonprofits only

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DTA in pCDEST2

Plasmid

#223705

Purpose

Mammalian expression vector encoding diptheria toxin A

Depositor

Rob Parton

Article

Luong et al ACS Nano. 2025 Dec 23;19(50):42079-42096

Insert

Diphtheria toxin A

Expression

Mammalian

Available Since

Oct. 3, 2024

Availability

Academic Institutions and Nonprofits only

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ReU_pET29b

Plasmid

#215426

Purpose

Expression construct for ReU gene, codon optimized for expression in E. coli

Depositor

Gregg Beckham

Article

Bell et al Nat Commun. 2024 Feb 9;15(1):1217. doi:

Insert

ReU

Expression

Bacterial

Available Since

April 29, 2024

Availability

Academic Institutions and Nonprofits only

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pYSD085

Plasmid

#212922

Purpose

Encodes the HA-tagged 649 Stalk yeast surface display anchor protein as a Type 4a part to be used in the yeast secretion/display (YSD) extension of the MoClo yeast toolkit (YTK)

Depositor

Paul Young

Article

O'Riordan et al ACS Synth Biol. 2024 Mar 14. doi: 10.102

Insert

649 Stalk

Expression

Bacterial

Available Since

Feb. 5, 2024

Availability

Academic Institutions and Nonprofits only

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GWF058

Plasmid

#133609

Purpose

UAS-mCherry/CMV-Gal4DBD-NS3a-P2a-DNCR2-VPR

Depositor

Dustin Maly

Article

Foight et al Nat Biotechnol. 2019 Sep 9. pii: 10.1038

Insert

Gal4DBD-NS3a, DNCR2-VPR

Expression

Mammalian

Available Since

Oct. 30, 2019

Availability

Academic Institutions and Nonprofits only

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p276 eSpCas9_2gRNAs_hH11

Plasmid

#164850

Purpose

gRNA vector for targeting human H11 locus

Depositor

Philip Jordan

Article

Pryzhkova et al Tissue Eng Regen Med. 2020 Apr;17(2):223

Insert

gRNAs for targeting human H11 locus

Expression

Mammalian

Promoter

U6

Available Since

March 9, 2021

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-hNox4

Plasmid

#69352

Purpose

expresses human Nox4 in mammalian cells

Depositor

Karl-Heinz Krause

Article

Serrander et al Biochem J. 2007 Aug 15;406(1):105-14.

Insert

hNOX4 (NOX4 Human)

Expression

Mammalian

Promoter

CMV

Available Since

March 15, 2016

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.S29W.A95K.mCherry

Plasmid

#248069

Purpose

iATPSnFR2.S29W.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.S29W.A95K.mCherry

Use

AAV

Mutation

S29W.A95K

Available Since

March 5, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95A.A119L.mCherry

Plasmid

#248073

Purpose

iATPSnFR2.A95A.A119L variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95A.A119L.mCherry

Use

AAV

Mutation

A95A.A119L

Available Since

March 3, 2026

Availability

Academic Institutions and Nonprofits only

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TET1cd-dCas9 (D12)

Plasmid

#239488

Purpose

Encodes a CRISPR/dCas9-based fusion protein designed for targeted DNA demethylation.

Depositor

Jian-Kang Zhu

Article

He et al Nat Commun. 2025 Dec 6. doi: 10.1038/s41

Insert

RPS5A-TET1cd-XTEN80-NLS-dCas9-2xNLS-P2A-BFP

Use

CRISPR

Expression

Plant

Available Since

Feb. 17, 2026

Availability

Academic Institutions and Nonprofits only

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pAAV.CAG.(cyto).iATPSnFR2.A95K.mCherry

Plasmid

#248071

Purpose

iATPSnFR2.A95K variant with non-responsive mCherry

Depositor

Tim Brown

Article

Marvin et al Proc Natl Acad Sci U S A. 2024 May 21;12

Insert

iATPSnFR2.A95K.mCherry

Use

AAV

Mutation

A95K

Available Since

Jan. 8, 2026

Availability

Academic Institutions and Nonprofits only

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pHR-DDX4N-mCh-Cry2WT

Plasmid

#101225

Purpose

DDX4(1-236) fused to mCh-Cry2WT

Depositor

Clifford Brangwynne

Article

Shin et al Cell. 2017 Jan 12;168(1-2):159-171.e14.

Insert

DDX4(1-236)

Use

Lentiviral

Expression

Mammalian

Promoter

SFFV

Available Since

Feb. 1, 2018

Availability

Academic Institutions and Nonprofits only

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2XMyc-LRRK2-WT

Plasmid

#25361

Depositor

Mark Cookson

Article

J Biol Chem. 2008 Jun 13;283(24):16906-1

Insert

LRRK2 (LRRK2 Human)

Tags

2XMyc

Expression

Mammalian

Available Since

June 17, 2010

Availability

Academic Institutions and Nonprofits only

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pDYU1G/mRuby3–histone H1; γ-tubulin-mEGFP

Plasmid

#154303

Purpose

Expresses mRuby3–histone H1 and γ-tubulin-mEGFP from actin15 promoter in Dictyostelium discoideum

Depositor

Shigehiko Yumura

Article

Kondo et al Biotechniques. 2020 Feb;68(2):91-95. doi

Inserts

histone H1
γ-tubulin

Use

Dictyostelium expression

Tags

mEGFP and mRuby3

Promoter

actin15

Available Since

July 6, 2021

Availability

Academic Institutions and Nonprofits only

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Halo-Cyto

Plasmid

#124314

Purpose

Expresses cytosolic specific HaloTag protein

Depositor

Jin Wang

Article

Jiang et al Antioxid Redox Signal. 2018 Oct 25. doi:

Insert

Halo tag

Tags

Cytoplasm specific seq

Expression

Mammalian

Promoter

CMV

Available Since

May 16, 2019

Availability

Academic Institutions and Nonprofits only

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pLexM-SLC7A11

Plasmid

#197993

Purpose

Expresses SLC7A11 with an N-terminal FLAG tag in mammalian cells

Depositor

Simon Newstead

Article

Parker et al Nat Commun. 2021 Dec 8;12(1):7147. doi:

Insert

SLC7A11 (SLC7A11 Human)

Tags

FLAG tag

Expression

Mammalian

Promoter

CMV

Available Since

March 27, 2023

Availability

Academic Institutions and Nonprofits only

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pDisplay-GRAB_ATP1.0-IRES-mCherry-CAAX

Plasmid

#167582

Purpose

Express the ATP sensor GRAB_ATP1.0 in mammalian cells

Depositor

Yulong Li

Article

Wu et al Neuron. 2021 Dec 17. pii: S0896-6273(21)

Insert

ATP sensor GRAB_ATP1.0 (P2RY1 Human)

Expression

Mammalian

Promoter

CMV

Available Since

April 21, 2021

Availability

Academic Institutions and Nonprofits only

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PAX6 donor plasmid

Plasmid

#105239

Purpose

Knock in H2B-GFP into the human PAX6 locus

Depositor

Lorenz Studer

Article

Tchieu et al Cell Stem Cell. 2017 Sep 7;21(3):399-410

Insert

PAX6 Homologous recombination ARMS with a P2A-H2B-GFP and Puromycin selection cassette

Use

TALEN

Available Since

May 31, 2018

Availability

Academic Institutions and Nonprofits only

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