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pDN-D2irTNG5kwh

Plasmid

#128279

Purpose

Mammalian linearizer gene circuit in a Flp-In expression system with a hTetR::EGFP fusion protein

Depositor

Gabor Balazsi

Article

Farquhar et al Nat Commun. 2019 Jun 24;10(1):2766. doi:

Insert

hTetR::NLS::EGFP

Use

Synthetic Biology; Flp-in expression vector

Tags

EGFP

Expression

Mammalian

Promoter

CMV-D2ir promoter

Available Since

Jan. 2, 2020

Availability

Academic Institutions and Nonprofits only

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pHBDuet139

Plasmid

#121913

Purpose

Charge-Introduced NpuDnaB mini-intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

CI-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pHBDuet140

Plasmid

#121914

Purpose

Orthogonal NpuDnaB mini-intein intein

Depositor

Hideo Iwai

Article

Beyer et al FEBS J. 2019 Oct 30. doi: 10.1111/febs.1

Insert

Oth-NpuDnaBΔ290 intein

Tags

GB1 and His6-GB1

Expression

Bacterial

Mutation

Deletion of 290-residue endonuclease domain, I53K…

Promoter

T7

Available Since

Dec. 2, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0747

Plasmid

#133481

Purpose

Expression in fission yeast from promoter ppom1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pom1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0748

Plasmid

#133482

Purpose

Expression in fission yeast from promoter ppak1 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(pak1)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0605

Plasmid

#133503

Purpose

Expression of sfGFP targeted to the plasma membrane with an amphipathic helix of RitC

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(act1)-sfGFP-RitC-terminator(tdh1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pAV0746

Plasmid

#133480

Purpose

Expression in fission yeast from promoter prga3 , integrated at ura4 locus

Depositor

Sophie Martin

Article

Vjestica et al J Cell Sci. 2020 Jan 8;133(1). pii: jcs.

Insert

pUra4(AfeI)-p(rga3)-sfGFP-terminator(ScCYC1)

Expression

Yeast

Available Since

Nov. 20, 2019

Availability

Academic Institutions and Nonprofits only

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pFA-dabAB2

Plasmid

#133012

Purpose

pFA carrying the badabA2 (locus_tag: BAS2958) and badabB2 genes (locus_tag: BAS2959)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-dabAB2 C351A

Plasmid

#133005

Purpose

pFE carrying a dabA2 gene with a C351A mutation and a dabB2 gene (Uniprot: D0KWS8)

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

dabB2
dabA2

Expression

Bacterial

Mutation

C351A

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pFE-baDAB

Plasmid

#133003

Purpose

pFE carrying the badabA and badabB genes

Depositor

David Savage

Article

Desmarais et al Nat Microbiol. 2019 Aug 12. pii: 10.1038

Inserts

badabB
vcdabA

Expression

Bacterial

Promoter

tetR

Available Since

Nov. 11, 2019

Availability

Academic Institutions and Nonprofits only

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pQlinkH-YbATP-grasp

Plasmid

#129639

Purpose

Expression plasmid of ATP-grasp from Y. bercovieri

Depositor

Matthias Bochtler

Article

Piasecka et al J Mol Biol. 2018 Mar 2;430(5):611-627. d

Insert

YbATP-grasp

Tags

His6-tag

Expression

Bacterial

Promoter

pQE

Available Since

Nov. 4, 2019

Availability

Academic Institutions and Nonprofits only

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pGEX-4T1 mouse Tankyrase1 ARC2-3 (308-655)

Plasmid

#132637

Purpose

expresses mouse Tankyrase1 ARC2-3 (308-655) in E. coli

Depositor

Wenqing Xu

Article

Morrone et al Proc Natl Acad Sci U S A. 2012 Jan 31;10

Insert

Tankyrase1 (308-655)

Expression

Bacterial

Available Since

Oct. 17, 2019

Availability

Academic Institutions and Nonprofits only

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FH95pUP95S73AGW (F2)

Plasmid

#74039

Purpose

lentiviral expression of Psd95 shRNA and Psd95-S73A-EGFP fusion

Depositor

Robert Malenka, Bernardo Sabatini, Weifeng Xu

Article

Steiner et al Neuron. 2008 Dec 10;60(5):788-802. doi:

Insert

Psd95

Use

Lentiviral and RNAi

Expression

Mammalian

Mutation

S73A

Promoter

H1

Available Since

Aug. 6, 2019

Availability

Academic Institutions and Nonprofits only
pRS-ATG1(mut)(406)

Plasmid

#122069

Purpose

Expressing a mutant ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

Codon changes without changing the amino acid seq…

Available Since

April 9, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-ATG7p-ATG1(406)

Plasmid

#122071

Purpose

Expressing ATG1

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

ATG1 (ATG1 Budding Yeast)

Expression

Yeast

Mutation

The native promoter was replaced by ATG7 promoter…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(D195A,E196A)(405)

Plasmid

#122065

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Aspartic acid 195 to alanine; Glutamic acid 196 t…

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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pRS-Dhh1-PA(S226A,T228A)(405)

Plasmid

#122066

Purpose

Expressing Dhh1-PA

Depositor

Daniel Klionsky

Article

Liu et al PLoS Biol. 2019 Apr 11;17(4):e3000219. d

Insert

Dhh1-PA (DHH1 Budding Yeast)

Tags

PA

Expression

Yeast

Mutation

Serine 226 to alanine; Threonine 228 to alanine

Available Since

April 2, 2019

Availability

Academic Institutions and Nonprofits only

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mdm1_pJet1.2

Plasmid

#121977

Purpose

for generating mdm1 riboprobes for WISH in zebrafish

Depositor

GuangJun Zhang

Article

Hensley et al PLoS One. 2016 Sep 22;11(9):e0163229. do

Insert

mdm1 (mdm1 Zebrafish)

Use

Cloning vector

Promoter

T7

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pRPL28 CaURA3 (pNTI592)

Plasmid

#115431

Purpose

URA3-marked RPS28

Depositor

Nicholas Ingolia

Article

McGeachy et al ACS Synth Biol. 2019 Apr 19;8(4):844-856

Inserts

RPL28 (RPL28 Budding Yeast)
URA3

Expression

Yeast

Mutation

KpnI site in intron

Promoter

Ashbya gossypii and endogenous

Available Since

Nov. 26, 2018

Availability

Academic Institutions and Nonprofits only

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pEPOR0SP0012

Plasmid

#117533

Purpose

Level0 Golden Gate part: CDS, eSaCas9 (no stop codon)

Depositor

Nicola Patron

Article

Raitskin et al PLoS One. 2019 Feb 27;14(2):e0211598. do

Insert

eSaCas9 (no stop codon)

Use

CRISPR

Mutation

R499A,Q500K,R654A,G655R

Available Since

Nov. 13, 2018

Availability

Academic Institutions and Nonprofits only

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