We narrowed to 8,725 results for: Set

1. ZJOM43

   Plasmid

   #133652

   Purpose

   Integration of VPS4-DuDre as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-DuDre (VPS4 Budding Yeast)
   

   Tags

   DuDre

   Expression

   Yeast

   Available Since

   Aug. 10, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. EGFP-BAF_L58R

   Plasmid

   #101776

   Purpose

   Expresses EGFP tagged mutant BAF (L58R) in human cells

   Depositor

   Daniel Gerlich

   Article

   Samwer et al Cell. 2017 Aug 24;170(5):956-972.e23. do

   Insert

   BAF (BANF1) (BANF1 Human)
   

   Use

   Lentiviral

   Tags

   EGFP

   Expression

   Mammalian

   Mutation

   L58R mutation

   Promoter

   EF1a

   Available Since

   Jan. 8, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pLEX305-N-dTAG-CARD8

   Plasmid

   #169989

   Purpose

   Mammalian expression of the dTAG-CARD8 fusion protein for controllable activation with dTAG ligands (dTAG13, dTAGV-1)

   Depositor

   Daniel Bachovchin

   Article

   Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

   Insert

   CARD8 (CARD8 Human)
   

   Tags

   dTAG

   Expression

   Mammalian

   Promoter

   hPGK

   Available Since

   May 17, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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4. ZJOM44

   Plasmid

   #133653

   Purpose

   Integration of SNF7-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-SNF7-mCherry (SNF7 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. ZJOM10

   Plasmid

   #133639

   Purpose

   Integration of VPS4-GFP as an additional copy in genome, use auxotrophic marker URA3(Candida albicans). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-Vps4-GFP (VPS4 Budding Yeast)
   

   Tags

   EGFP

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. ZJOM70

   Plasmid

   #133658

   Purpose

   Integration of ERG6-mCherry as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast lipid droplet.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ERG6-mCherry (ERG6 Budding Yeast)
   

   Tags

   mCherry

   Expression

   Yeast

   Available Since

   Feb. 12, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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7. ZJOM136

   Plasmid

   #133660

   Purpose

   Integration of ELO3-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast endoplasmic reticulum (ER).

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-ELO3-mTagBFP2 (ELO3 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Dec. 5, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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8. ZJOM113

   Plasmid

   #133666

   Purpose

   Integration of VPS4-mTagBFP2 as an additional copy in genome, use auxotrophic marker TRP1(Kluyveromyces lactis). Marker for yeast late endosome.

   Depositor

   Zhiping Xie

   Article

   Zhu et al MBio. 2019 Sep 3;10(5). pii: mBio.01691-

   Insert

   p1k-VPS4-mTagBFP2 (VPS4 Budding Yeast)
   

   Tags

   mTagBFP2

   Expression

   Yeast

   Available Since

   Jan. 14, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-NORAD

   Plasmid

   #196086

   Purpose

   Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting lncRNA NORAD.

   Depositor

   Joanna Bereta

   Article

   Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

   Insert

   gRNA targeting lncRNA NORAD
   

   Use

   CRISPR

   Expression

   Mammalian

   Mutation

   Cloned using SapI sites - destroyed after inserti…

   Available Since

   March 30, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pPyCAG-NE-mKate2-IRES-Hph

    Plasmid

    #183616

    Purpose

    Mammalian expression vector: CAG promoter driving expression of nuclear envelope-tethered mKate2 (NLS-mKate2-EmerinTMD). Confers hygromycin B resistance.

    Depositor

    Sally Lowell

    Article

    Blin et al PLoS Biol. 2019 Aug 9;17(8):e3000388. do

    Insert

    mKate2
    

    Tags

    Human EMD transmembrane domain and NLS

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 12, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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11. pSBtet-Pur-dCas9-KRAB-MeCP2_hU6-A10

    Plasmid

    #196085

    Purpose

    Inducible CRISPRi vector conferring puromycin resistance, expressing gRNA targeting mouse Adam10.

    Depositor

    Joanna Bereta

    Article

    Czarnek et al Mol Biotechnol. 2023 Jan 28. doi: 10.100

    Insert

    gRNA targeting Adam10
    

    Use

    CRISPR

    Expression

    Mammalian

    Mutation

    Cloned using SapI sites - destroyed after inserti…

    Available Since

    March 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. pcDNA3.1(+)Gαi1-91-NLuc

    Plasmid

    #207148

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAI1 tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAI1 (GNAI1 Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position L(91)K

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pcDNA3.1 LIC 6A CARD8-FLAG S297A

    Plasmid

    #169949

    Purpose

    Mammalian expression of CARD8-FLAG that is autoprocessing-deficient (S297A)

    Depositor

    Hao Wu

    Article

    Sharif et al Immunity. 2021 Jul 13;54(7):1392-1404.e1

    Insert

    CARD8 (CARD8 Human)
    

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    S297A

    Promoter

    CMV

    Available Since

    May 11, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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14. pcDNA3.1(+)Gαq-124-NLuc

    Plasmid

    #207153

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNAQ tagged with NanoLuciferase and is meant to be paired with Ggamma2 labeled with LSS-mKATE2

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNAQ (GNAQ Human)
    

    Expression

    Mammalian

    Mutation

    NLuc inserted at position F(124)E

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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15. pcDNA3.1(+)Gγ2-LSS-mKATE2(N)

    Plasmid

    #207160

    Purpose

    Part of REGA-SIGN, a NanoBRET-based G protein biosensor. It encodes GNG2 tagged with LSS-mKATE2 to be paired with one of the GNA

    Depositor

    Dominique Schols, Tom Van Loy

    Article

    Boon et al Biosensors (Basel). 2023 Jul 28;13(8):76

    Insert

    GNG2 (GNG2 Human)
    

    Expression

    Mammalian

    Mutation

    N-terminally tagged with LSS-mKATE2

    Available Since

    Nov. 20, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::HA-RfA

    Plasmid

    #186411

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion N-ter HA tag-gene of interest under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::venus-RfA

    Plasmid

    #186408

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter Venus under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Oct. 4, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pDEST-pSP172BSSPE-R3-ccdB/CmR-R5::RfA-CFP

    Plasmid

    #186412

    Purpose

    Multisite destination vector for ascidian electroporation of an AttB1/B2 recombined fusion gene of interest-C-ter CFP under control of an AttB3/B5 recombined reg. sequence.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Type

    Empty backbone

    Use

    Gateway destination vector

    Available Since

    Sept. 30, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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19. pENT-L1-Kozak-NLSlacZ-stop-L2

    Plasmid

    #186358

    Purpose

    Entry clone with ORF encoding NLS lacZ flanked by Gateway recombination sequences.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    lacZ (lacZ E. coli, Synthetic)
    

    Use

    Expression of nuclear lacz reporter

    Tags

    Nuclear localization signal

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENT-L1-Kozak-HistoneH2B-L2

    Plasmid

    #186357

    Purpose

    Entry clone with ORF encoding histone H2B flanked by Gateway recombination sequences. No Stop sequence: to be recombined into a destination vector with a C-terminal protein tag.

    Depositor

    Patrick Lemaire

    Article

    Roure et al PLoS One. 2007. 2(9):e916.

    Insert

    Histone H2B (H2BC11 Human)
    

    Use

    Expression of a tagged nuclear marker

    Available Since

    Sept. 28, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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