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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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We narrowed to 3,270 results for: Streptococcus

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Showing: 1081 - 1100 of 3270 results

  2. PX383 Lactobacillus johnsonii Cas9

    Plasmid
    #68320
    Purpose
    Lactobacillus johnsonii Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LjCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Sept. 28, 2015
    Availability
    Academic Institutions and Nonprofits only
    68320-plasmid-map-sequence-id-119535

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  3. pMuLE ENTR SV40-hCas9 L3-L2

    Plasmid
    #62133
    Purpose
    MuLE (Multiple Lentiviral Expression) Entry vector containing SV40 promoter and human codon optimized Cas9 module. Compatible with MultiSite Gateway cloning
    Depositor
    Ian Frew
    Publication
    Albers et al J Clin Invest. 2015 Mar 9. pii: 79743. d
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    human codon optimized Cas9
    Use
    CRISPR and Gateway Cloning
    Expression
    Mammalian
    Promoter
    SV40
    Available since
    June 16, 2015
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    62133-plasmid-map-sequence-id-101417

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  4. pYC2085

    Plasmid
    #158721
    Purpose
    CRISPR system used for genome editing in Mycobacterium tuberculosis. Helper plasmid expresses Sth1 Cas9 and the cognate sgRNA, using hygromycin as a selection marker.
    Depositor
    Yi-Cheng Sun
    Publication
    Yan et al mBio. 2020 Jan 28;11(1). pii: mBio.02364
    Insert
    Sth1 sgRNA scaffold
    Use
    CRISPR
    Expression
    Bacterial
    Available since
    Jan. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    158721-plasmid-map-sequence-id-320843

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  10. pCas9-beta-mmr

    Plasmid
    #169241
    Purpose
    Cas9, beta, and mutL mutant expression plasmid for introducing chromosomal point mutations
    Depositor
    Brian Pfleger
    Publication
    Cook et al Metab Eng. 2021 Jun 24;67:112-124. doi:
    Inserts
    cas9
    beta
    mutL-E36K
    Use
    CRISPR and Synthetic Biology
    Expression
    Bacterial
    Mutation
    Changed glutamic acid 36 to lysine
    Available since
    June 16, 2021
    Availability
    Academic Institutions and Nonprofits only
    169241-plasmid-map-sequence-id-333575

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  11. TMPRSS2 g4 + Cas9

    Plasmid
    #153018
    Purpose
    A guide RNA targeting TMPRSS2 in a lentiviral plasmid co-expressing Cas9 and TagBFP2
    Depositor
    Jason Sheltzer
    Insert
    Cas9 + TMPRSS2 gRNA
    Use
    CRISPR and Lentiviral
    Tags
    TagBFP2
    Available since
    June 2, 2020
    Availability
    Academic Institutions and Nonprofits only
    153018-plasmid-map-sequence-id-298698

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  13. pX330-HA-dSpCas9

    Plasmid
    #92112
    Purpose
    Expression plasmid for human codon-optimized dead/inactive SpCas9 (without U6-sgRNA coding sequence)
    Depositor
    Ervin Welker
    Publication
    Kulcsar et al Genome Biol. 2017 Oct 6;18(1):190. doi:
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dead/inactive SpCas9
    Use
    CRISPR
    Tags
    HA tag and NLS
    Expression
    Mammalian
    Mutation
    D10A, H840A
    Promoter
    Cbh
    Available since
    Oct. 30, 2017
    Availability
    Academic Institutions and Nonprofits only
    92112-plasmid-map-sequence-id-197503

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  14. FUCas9Cyan

    Plasmid
    #85554
    Purpose
    Expresses Cas9 with fluorescent Cyan reporter
    Depositor
    Marco Herold
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    humanised S.pyrogenes Cas9
    Use
    CRISPR
    Tags
    3xFLAG
    Expression
    Mammalian
    Promoter
    hUBC
    Available since
    June 9, 2017
    Availability
    Academic Institutions and Nonprofits only
    85554-plasmid-map-sequence-id-180892

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  15. PX386 Lactobacillus farciminis Cas9

    Plasmid
    #68323
    Purpose
    Lactobacillus farciminis Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    LfCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    68323-plasmid-map-sequence-id-119538

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  17. PX393 Gluconacetobacter diazotrophicus Cas9

    Plasmid
    #68330
    Purpose
    Gluconacetobacter diazotrophicus Cas9
    Depositor
    Feng Zhang
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    GdCas9
    Use
    CRISPR
    Tags
    HA and NLS
    Expression
    Mammalian
    Promoter
    CMV
    Available since
    Aug. 20, 2015
    Availability
    Academic Institutions and Nonprofits only
    68330-plasmid-map-sequence-id-119553

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  19. pBBR1.cas9-NT

    Plasmid
    #197871
    Purpose
    Constitutive expression of Cas9 endonuclease, trancRNA and crRNA with a randomly generated spacer sequence (non-targeting)
    Depositor
    Baojun Wang
    Publication
    Huan et al ACS Synth Biol. 2023 Mar 17;12(3):709-72
    Inserts
    trancCRNA
    Cas9
    crRNA (non-chromosomal (E. coli/S. flexneri)-targeting spacer sequence)
    Expression
    Bacterial
    Available since
    June 14, 2023
    Availability
    Academic Institutions and Nonprofits only
    197871-plasmid-map-sequence-id-396456

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  20. XClone_Cas9_KRAB_T2A_BFP

    Plasmid
    #141208
    Purpose
    Tunable and temporal expression control of dCas9
    Depositor
    Rory Johnson
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    dCas9
    Expression
    Mammalian
    Available since
    Aug. 18, 2020
    Availability
    Academic Institutions and Nonprofits only
    141208-plasmid-map-sequence-id-308770

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Showing: 1081 - 1100 of 3270 results