We narrowed to 1,127 results for: fret

1. eZiPro W69F

   Plasmid

   #198438

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W69F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. eZiPro W89F

   Plasmid

   #198440

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W89F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. gZiPro W69F

   Plasmid

   #198445

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W69F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. eZiPro

   Plasmid

   #198434

   Purpose

   Activity/inhibition assays, 19F-NMR

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. eZiPro W61*F

   Plasmid

   #198435

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W61*F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. eZiPro W50F

   Plasmid

   #198437

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   enzymatically cleavable linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   W50F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. gZiPro W50F

   Plasmid

   #198444

   Purpose

   Activity/inhibition assays, 19F-NMR assignment

   Depositor

   Tanja Schirmeister, Ute Hellmich

   Article

   Maus et al Eur J Med Chem. 2023 Oct 5;258:115573. d

   Insert

   glycine linked Zika virus NS2B-NS3
   

   Use

   Tags

   His6-TEV

   Expression

   Bacterial

   Mutation

   R95*A, W50F

   Promoter

   T7 promoter

   Available since

   March 29, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pcDNA3-Venus-cpVenus-FLARE-Cameleon

   Plasmid

   #123350

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based genetically encoded calcium indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-Cameleon
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   July 28, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pcDNA3-Venus-cpVenus-FLARE-ICUE

   Plasmid

   #123355

   Purpose

   Yellow-fluorescent homoFRET/anisotropy-based cAMP indicator. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

   Depositor

   Jin Zhang

   Article

   Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

   Insert

   Venus-cpVenus-FLARE-ICUE
   

   Use

   Tags

   6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Aug. 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pcDNA3-Venus-cpVenus-FLARE-AKAR

    Plasmid

    #123329

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-AKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Sept. 29, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pcDNA3-Venus-cpVenus-FLARE-EKAR-EV

    Plasmid

    #123339

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring ERK activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-EKAR-EV
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. pcDNA3-Venus-cpVenus-FLARE-CKAR

    Plasmid

    #123343

    Purpose

    Yellow-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase C activity. Contains monomeric Venus/cpVenus-E172 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Venus-cpVenus-FLARE-CKAR
    

    Use

    Tags

    6xHIS, T7 tag (gene 10 leader), Venus, Xpress (TM…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pcDNA3-Cerulean3-cpCerulean3-FLARE-AKAR

    Plasmid

    #123336

    Purpose

    Cyan-fluorescent homoFRET/anisotropy-based biosensor for monitoring Protein Kinase A activity. Contains monomeric Cerulean3/cpCerulean3-E173 homoFRET pair.

    Depositor

    Jin Zhang

    Article

    Ross et al eLife. 2018 Jul 3;7. pii: 35458. doi: 10

    Insert

    Cerulean3-cpCerulean3-FLARE-AKAR
    

    Use

    Tags

    6xHIS, Cerulean3, T7 tag (gene 10 leader), Xpress…

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. PB-NES-ZapCV2

    Plasmid

    #222950

    Purpose

    Genetically-encoded Cytosolic Cyan-Yellow Zinc FRET sensor. Useful for detecting free zinc ion levels in the cytosol (in vitro Kd ~2.3 nM, n = 0.53)

    Depositor

    Amy Palmer

    Article

    Fiedler et al Anal Chem. 2017 Jan 3;89(1):711-719. doi

    Insert

    NES-ZapCV2 (NES Synthetic)
    

    Use

    Tags

    ECFP (Enhanced Cyan Fluorescent Protein), Nuclear…

    Expression

    Mammalian

    Mutation

    ECFP gene is truncated 36 bp at 3' end. Venu…

    Promoter

    Available since

    Nov. 12, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pLV x EF1a_EKAREN4-ires-blast

    Plasmid

    #167829

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pLV x EF1a_EKAREN5-ires-blast

    Plasmid

    #167822

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. TdLanYFP-AURKA-Aquamarine

    Plasmid

    #228562

    Purpose

    Encodes the AURKA biosensor where LC3B is flanked by the Aquamarine/TdLanYFP donor/acceptor FRET pair and under the control of the CMV promoter.

    Depositor

    Giulia Bertolin

    Article

    Bousmah et al ACS Sens. 2021 Nov 26;6(11):3940-3947. d

    Insert

    AURKA (AURKA, AURKA Human)
    

    Use

    Tags

    Aquamarine and TdLanYFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Jan. 9, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pLV x EF1a_EKAREN5-ires-puro

    Plasmid

    #167821

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN5
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    Aug. 4, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pcDNA3-ICUE4

    Plasmid

    #181846

    Purpose

    Fourth-generation FRET-based Indicator of cAMP Using Epac. Genetically encoded cAMP indicator. Contains high-sensitivity Epac Q270E mutation.

    Depositor

    Jin Zhang

    Article

    Zhang et al Cell. 2020 Sep 17;182(6):1531-1544.e15.

    Insert

    ICUE4 (RAPGEF3 Human)
    

    Use

    Tags

    6xHIS - T7 tag (gene 10 leader) - Xpress (TM) tag…

    Expression

    Mammalian

    Mutation

    Glutamine 122 in Epac1 domain mutated to glutamat…

    Promoter

    CMV

    Available since

    June 17, 2022

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pLV x EF1a_EKAREN4-ires-puro

    Plasmid

    #167828

    Purpose

    Optimized EKAREV FRET biosensor sensor for ERK

    Depositor

    Hugo Snippert

    Article

    Ponsioen et al Nat Cell Biol. 2021 Apr;23(4):377-390. d

    Insert

    EKAREN4
    

    Use

    Lentiviral

    Tags

    nls localization motif

    Expression

    Mammalian

    Mutation

    K424P, K426W

    Promoter

    EF1a

    Available since

    April 20, 2021

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge